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Scramble nonspecific sirna

Manufactured by Santa Cruz Biotechnology
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Scramble nonspecific siRNA is a laboratory reagent used to create a control sample in RNA interference (RNAi) experiments. It does not target any specific gene or transcript and serves as a negative control to help evaluate the effects of gene silencing by small interfering RNA (siRNA).

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Lab products found in correlation

Mouse sip2x7 cat no sc 42576, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

SiRNAs (95 citations) Scramble siRNA (46 citations) Nonspecific siRNA (12 citations)

2 protocols using scramble nonspecific sirna

1

siRNA Knockdown of P2X7 and AMPK in BV-2 Cells

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Mouse siP2X7 (Cat no. SC-42576), siAMPK (Cat. no. SC45313) and scramble nonspecific siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). BV-2 microglial cells at 50% confluence were transfected with 100 nM siRNA by DharmaFECT Transfection Reagents (Dharmacon Research) following the manufacturer’s instruction. After 48 h transfection, cells were treated with indicated drugs and then harvested for analysis.
Sekar P., Huang D.Y., Hsieh S.L., Chang S.F, & Lin W.W. (2018). AMPK-dependent and independent actions of P2X7 in regulation of mitochondrial and lysosomal functions in microglia. Cell Communication and Signaling : CCS, 16, 83.
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2

Silenicing P2X7 Receptor in BV-2 Cells

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Mouse siP2X7 (Cat no. SC-42576) and scramble nonspecific siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). BV-2 cells were transfected with 100 nM siRNA by DharmaFECT Transfection Reagents (Dharmacon Research, Lafayette, CO, USA) at 50% confluence following the manufacturer's instruction. After 48 h of siRNA transfection, cells were treated with indicated drugs and then harvested for analysis.
Sekar P., Huang D.Y., Chang S.F, & Lin W.W. (2018). Coordinate effects of P2X7 and extracellular acidification in microglial cells. Oncotarget, 9(16), 12718-12731.
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