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Anti ac histone h4

Manufactured by Santa Cruz Biotechnology

Anti-Ac Histone H4 is a laboratory reagent used in various research applications. It is an antibody that specifically recognizes acetylated histone H4, a post-translational modification involved in regulating gene expression. This product can be utilized in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and immunohistochemistry to study epigenetic mechanisms and chromatin dynamics.

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2 protocols using anti ac histone h4

1

Histone Acetylation Antibody Validation

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Antibodies used for either immunoprecipitation or immunoblotting were Anti BRD4 (H-250) :sc-48772 and anti-Ac Histone H4: sc-34263 (Santa Cruz Biotech), anti-Ac Histone H3K4 (#07-539; Millipore), anti-Ac Histone H3 (#06-599; Upstate), anti-Ac Histone H3K9, H3K14, H3K18, H3K27, H3K56, anti-Histone H3 (from #9927 kit, Cell Signaling) and anti-Histone H4 (F-9: Santa Cruz Biotech), anti-Ac Histone H4K5, H4K8, H4K12, and H4K16 (from #17-211 kit, Millipore), anti-Tubulin (ab6046; Abcam), CBP (A-22: sc-369, Santa Cruz Biotech), p300 (3G230/NM-11, ab-14984, Abcam) and anti-Ac Histone H3K122 (ab33309; Abcam). The validation of specificity of the anti-Ac Histone H3K122 antibody (Lot #436839 and #GR14667-1) and other antibodies used in the study are provided on the manufacturers’ websites. HAT inhibitors Anacardic acid, Curcumin and Garcinol (Biovision) and C646 (Sigma) were dissolved in dimethyl sulfoxide (DMSO) to make concentrated stock solutions. Stock solutions of all HAT inhibitors were stored at −80°C. The (+)-JQ1 inhibitor (BPS Bioscience) was similarly dissolved in DMSO and stored.
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2

Histone Acetylation Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used for either immunoprecipitation or immunoblotting were Anti BRD4 (H-250) :sc-48772 and anti-Ac Histone H4: sc-34263 (Santa Cruz Biotech), anti-Ac Histone H3K4 (#07-539; Millipore), anti-Ac Histone H3 (#06-599; Upstate), anti-Ac Histone H3K9, H3K14, H3K18, H3K27, H3K56, anti-Histone H3 (from #9927 kit, Cell Signaling) and anti-Histone H4 (F-9: Santa Cruz Biotech), anti-Ac Histone H4K5, H4K8, H4K12, and H4K16 (from #17-211 kit, Millipore), anti-Tubulin (ab6046; Abcam), CBP (A-22: sc-369, Santa Cruz Biotech), p300 (3G230/NM-11, ab-14984, Abcam) and anti-Ac Histone H3K122 (ab33309; Abcam). The validation of specificity of the anti-Ac Histone H3K122 antibody (Lot #436839 and #GR14667-1) and other antibodies used in the study are provided on the manufacturers’ websites. HAT inhibitors Anacardic acid, Curcumin and Garcinol (Biovision) and C646 (Sigma) were dissolved in dimethyl sulfoxide (DMSO) to make concentrated stock solutions. Stock solutions of all HAT inhibitors were stored at −80°C. The (+)-JQ1 inhibitor (BPS Bioscience) was similarly dissolved in DMSO and stored.
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