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3 protocols using tertiary butanol

1

Versatile Hydrogel Synthesis Protocols

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Kevlar 1000D was obtained
from Dongguan SOVETL
Co., Ltd. Potassium hydroxide (KOH), 1,4-dibromo butane, sodium alginate
and gelatin, hydroiodic acid, and Rhodamine 6G were purchased from
Aladdin Co. DMSO, ethanol, tertiary butanol, glycerol, ammonia aqueous
solution (NH3·H2O, 25–28 wt %),
sodium chloride (NaCl), calcium chloride dihydrate (CaCl2·2H2O), formic acid, and chitosan were purchased
from Sinopharm Chemical Reagent Co., Ltd. Carpobol 940 and U20 were
purchased from Guangzhou Bailijia Technology Co. Ltd., and l-ascorbic acid was purchased from Macklin Co., Ltd. Cotton balls
were kindly provided by Winner Medical Supplies Co., Ltd. 1-Allyl-3-methyllmldazollum
chloride was purchased from Energy Chemical Co. Graphene oxide (GO),
Ti3C2 MXene, and condensed silica (CS) colloidal
particles were synthesized in our group; cellulose nanofiber was kindly
provided by Jiangsu North Century Cellulose Material Co., Ltd. Deionized
water (18.2 MΩ cm–1) was obtained from a Millipore-Q
system. All other reagents were used without further purification.
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2

Synthesis and Characterization of PLCL Copolymers

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PLCL with two different molar ratios of L-lactide and ε-caprolactone copolymers (50: 50, Mw = 50 kDa and 75: 25, Mw = 25 kDa) were purchased from Daigang Biotech (Jinan, China). Trichloromethane, methanol, acetone, NaCl, tertiary butanol, isopropyl alcohol, 1,6-hexanediamine, formaldehyde, ethanol, glutaraldehyde, and paraformaldehyde were supplied by Sinopharm Chemical Reagent (Shanghai, China). Ninhydrin hydrate (98%) and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). KHIFSDDSSEK-Pra (KHI, Pra: L-C-propargylglycine) peptides were synthesized by Sangon Biotech (Shanghai, China). The water used in all the experiments was purified by a Milli-Q cycle purification system (Millipore, Bedford, MA, USA).
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3

Synthesis and Characterization of PLCL Scaffolds

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PLCL were synthesized from l-lactide and ε-caprolactone with a molar ratio of 50: 50 (Mw 90 kDa) and 75: 25 (Mw 35 kDa) (Jinan Daigang Biotech Co., Ltd., China). Tertiary butanol, trichloromethane, formaldehyde, isopropyl alcohol, GA, acetone, paraformaldehyde and 1,6-hexanediamine were obtained from Sinopharm Chemical Reagent Co., China. Ninhydrin hydrate (98%) and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) were obtained from Macklin Biochemical Co., Ltd. CQAASIKVAV peptides were customized from GL Biochem Ltd., China. Milli-Q water was used for all the experiments (Millipore, USA).
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