Quantification of apoptosis/necrosis was performed by Annexin V-FITC conjugate and propidium iodide (PI) staining (Immunological Sciences) followed by fluorescence-activated cell sorting (FACS) performed with a FACScalibur flow cytometer equipped with a 488-nm argon laser (Becton Dickinson). The collected data were evaluated by the Cell Quest software. In addition, active caspase-3 and PARP-1 proteins were detected by conventional Western blot on total cell lysates prepared in SDS-PAGE sample buffer (62 mM Tris-HCl pH 6.8, 2% sodium dodecyl sulfate (SDS), 10% glycerol, 5% 2-mercaptoethanol, and 0.04% bromophenol blue). Densitometric analyses of immunoblots were performed using the National Institute of Health (NIH) ImageJ software package. Full-length unedited blots are shown in Additional file 4 .
Annexin 5 fitc conjugate and propidium iodide staining
Manufactured by Immunological Sciences
The Annexin V-FITC conjugate and propidium iodide (PI) staining are fluorescent dyes used for the detection and analysis of apoptosis and cell death in cell samples. Annexin V-FITC binds to phosphatidylserine, which is exposed on the cell surface during apoptosis. Propidium iodide is a nuclear stain that penetrates the membranes of dead or dying cells. Together, they provide a means to differentiate between viable, apoptotic, and necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using annexin 5 fitc conjugate and propidium iodide staining
1
Quantification of Apoptosis and Necrosis
2
Apoptosis and Necrosis Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quanti cation of apoptosis/necrosis was performed by Annexin V-FITC conjugate and propidium iodide (PI) staining (Immunological Sciences) followed by uorescence-activated cell sorting (FACS) performed with a FACScalibur ow cytometer equipped with a 488 nm argon laser (Becton Dickinson). The collected data were evaluated by Cell Quest software. In addition, active caspase-3 and PARP-1 proteins were detected by conventional Western blot on total cell lysates prepared in SDS-PAGE sample buffer (62 mM Tris-HCl pH 6.8, 2% sodium dodecyl sulfate (SDS), 10% glycerol, 5% 2-mercaptoethanol, and 0.04% bromophenol blue). Densitometric analyses of immunoblots were performed using the National Institute of Health (NIH) Image J software package. Full-length unedited blots are shown in the Additional le 4.
3
Quantification of Apoptosis and Necrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quanti cation of apoptosis/necrosis was performed by Annexin V-FITC conjugate and propidium iodide (PI) staining (Immunological Sciences) followed by uorescence activated cell sorting (FACS) performed with a FACScalibur ow cytometer equipped with a 488 nm argon laser (Becton Dickinson). The collected data were evaluated by Cell Quest software. In addition, active caspase-3 and PARP-1 proteins were detected by conventional western blot on total cell lysates prepared in SDS-PAGE sample buffer (62 mM Tris-HCl pH 6.8, 2% sodium dodecyl sulfate (SDS), 10% glycerol, 5% 2-mercaptoethanol, and 0.04% bromophenol blue). Densitometric analyses of immunoblots were performed using the National Institute of Health (NIH) Image J software package. Full length unedited blots are shown in the Additional le 2.
4
Quantification of Apoptosis and Necrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quanti cation of apoptosis/necrosis was performed by Annexin V-FITC conjugate and propidium iodide (PI) staining (Immunological Sciences) followed by uorescence activated cell sorting (FACS) performed with a FACScalibur ow cytometer equipped with a 488 nm argon laser (Becton Dickinson). The collected data were evaluated by Cell Quest software. In addition, active caspase-3 and PARP-1 proteins were detected by conventional western blot on total cell lysates prepared in SDS-PAGE sample buffer (62 mM Tris-HCl pH 6.8, 2% sodium dodecyl sulfate (SDS), 10% glycerol, 5% 2-mercaptoethanol, and 0.04% bromophenol blue). Densitometric analyses of immunoblots were performed using the National Institute of Health (NIH) Image J software package. Full length unedited blots are shown in the Additional le 4.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!