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Plan apo 0.75 n objective

Manufactured by Nikon
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The 10X Plan Apo 0.75 N objective is a high-performance microscope objective lens designed for a wide range of laboratory applications. It features a 10x magnification and a numerical aperture of 0.75, providing excellent optical performance and resolution. The 'Plan Apo' designation indicates that the lens is apochromatic, ensuring accurate color reproduction and minimal chromatic aberration.

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Lab products found in correlation

Orca flash 4.0 v2 cmos camera, by Hamamatsu Photonics (8 mentions) Plan apo 0.75 na objective, by Nikon (2 mentions) 24 well microplate, by Corning (2 mentions) Neutralized rat tail collagen 1, by Thermo Fisher Scientific (2 mentions) Peg di nhs, by MP Biomedicals (2 mentions) Matrigel, by Corning (2 mentions) Elements software, by Nikon (2 mentions) Matrigel, by Nikon (2 mentions)

Close spelling variants of this product

Plan Apo (135 citations) Plan Apo objective (51 citations)

8 protocols using plan apo 0.75 n objective

1

Mechanically-Conditioned Cell Spreading

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All cell lines and patient-derived xenografts were mechanically-conditioned for 36 hours on 0.5 kPa (Petrisoft) or 8.0 kPa (Petrisoft) hydrogels before analysis. Cells were imaged in situ using a 10X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu). Manual tracing was done to quantify cell spreading on each stiffness. After image acquisition, RT-qPCR analysis of CTGF was performed as detailed above.
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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2

Cell Adhesion and Spreading on Synthetic Bone Matrix

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Osteo Assay surface (synthetic bone matrix) 24-well microplates (Corning) were used. For adhesion, 24 hour-old preconditioned media from corresponding experimental groups were collected, and then 200 μL was pre-absorbed to the Osteo plates for 1 hour prior to adding 1.0 × 106 cells in 100 μL fresh media, and incubated for 30 min. Plates were gently tapped to remove loosely bound cells, and the remaining cells were stained with DAPI and enumerated by microscopy with large-stitch imaging using a 10X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu). For spreading measurements on synthetic bone matrix, the above protocol was used except imaging commenced immediately upon addition of cells to the plate. Cell area was quantified at 6 min intervals by manual tracing in Elements software (Nikon), using a 20X Plan Apo 0.75 NA objective (Nikon) and a CoolSNAP MYO CCD camera (Photometrics).
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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3

Mechanically-Conditioned Cell Spreading

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All cell lines and patient-derived xenografts were mechanically-conditioned for 36 hours on 0.5 kPa (Petrisoft) or 8.0 kPa (Petrisoft) hydrogels before analysis. Cells were imaged in situ using a 10X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu). Manual tracing was done to quantify cell spreading on each stiffness. After image acquisition, RT-qPCR analysis of CTGF was performed as detailed above.
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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4

Cell Adhesion and Spreading on Synthetic Bone Matrix

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Osteo Assay surface (synthetic bone matrix) 24-well microplates (Corning) were used. For adhesion, 24 hour-old preconditioned media from corresponding experimental groups were collected, and then 200 μL was pre-absorbed to the Osteo plates for 1 hour prior to adding 1.0 × 106 cells in 100 μL fresh media, and incubated for 30 min. Plates were gently tapped to remove loosely bound cells, and the remaining cells were stained with DAPI and enumerated by microscopy with large-stitch imaging using a 10X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu). For spreading measurements on synthetic bone matrix, the above protocol was used except imaging commenced immediately upon addition of cells to the plate. Cell area was quantified at 6 min intervals by manual tracing in Elements software (Nikon), using a 20X Plan Apo 0.75 NA objective (Nikon) and a CoolSNAP MYO CCD camera (Photometrics).
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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5

Cytoskeletal Dynamics in Breast Cancer Cells

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After mechanical preconditioning, SUM159, SKBR3 and MS-SKBR3.1 cells expressing iRFP-LifeAct were trypsizined from their hydrogels and plated onto No. 1.5 glass MatTek dishes which had been pre-treated overnight with DMEM + 10% FBS, and then incubated for 10 hours to ensure maximal spreading before analysis (verified by size equilibrium). Cytoskeletal dynamics score was obtained by automatic tracing of iRFP signal and averaging single-cell displacement over three sequential 1 hour intervals. Imaging was acquired with a 20X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu).
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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6

Cytoskeletal Dynamics in Breast Cancer Cells

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After mechanical preconditioning, SUM159, SKBR3 and MS-SKBR3.1 cells expressing iRFP-LifeAct were trypsizined from their hydrogels and plated onto No. 1.5 glass MatTek dishes which had been pre-treated overnight with DMEM + 10% FBS, and then incubated for 10 hours to ensure maximal spreading before analysis (verified by size equilibrium). Cytoskeletal dynamics score was obtained by automatic tracing of iRFP signal and averaging single-cell displacement over three sequential 1 hour intervals. Imaging was acquired with a 20X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu).
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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7

3D Invasion Assay for Breast Cancer Cells

Cited 1 time
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The invasion assays were modified from Padilla-Rodriguez et al. (2018) (link). Briefly, for SUM159 experiments, 75,000 preconditioned single cells were suspended in a dome of 15 μL Matrigel (Corning), spotted onto silanized 8-well coverslip chamber slides (LabTek), incubated for 30 min, and then embedded in 1 mg/mL neutralized rat tail collagen-I (Fisher) crosslinked with 0.0125% PEG-di(NHS) (MP Biomedicals) (see Figure 1A). Imaging was performed in a 16 hours period, starting 18 hours after embedding. For SKBR3 and MS-SKBR3.1 experiments 180,000 cells were suspended and embedded as above, and then imaging was performed immediately for 24 hours. Invaded cells which divided during the imaging periods were counted as one cell in order to mitigate any differences in proliferation among experimental groups. Invasion front translocation was calculated by tracking the midpoint of the cluster of cells at the Matrigel:collagen interface which align perpendicular to the direction of movement using DIC cell tracking in Elements software (Nikon). Imaging was acquired with a 20X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu).
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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8

3D Invasion Assay for Breast Cancer Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The invasion assays were modified from Padilla-Rodriguez et al. (2018) (link). Briefly, for SUM159 experiments, 75,000 preconditioned single cells were suspended in a dome of 15 μL Matrigel (Corning), spotted onto silanized 8-well coverslip chamber slides (LabTek), incubated for 30 min, and then embedded in 1 mg/mL neutralized rat tail collagen-I (Fisher) crosslinked with 0.0125% PEG-di(NHS) (MP Biomedicals) (see Figure 1A). Imaging was performed in a 16 hours period, starting 18 hours after embedding. For SKBR3 and MS-SKBR3.1 experiments 180,000 cells were suspended and embedded as above, and then imaging was performed immediately for 24 hours. Invaded cells which divided during the imaging periods were counted as one cell in order to mitigate any differences in proliferation among experimental groups. Invasion front translocation was calculated by tracking the midpoint of the cluster of cells at the Matrigel:collagen interface which align perpendicular to the direction of movement using DIC cell tracking in Elements software (Nikon). Imaging was acquired with a 20X Plan Apo 0.75 N objective (Nikon) and an ORCA-Flash 4.0 V2 cMOS camera (Hamamatsu).
Watson A.W., Grant A.D., Parker S.S., Hill S., Whalen M.B., Chakrabarti J., Harman M.W., Roman M.R., Forte B.L., Gowan C.C., Castro-Portuguez R., Stolze L.K., Franck C., Cusanovich D.A., Zavros Y., Padi M., Romanoski C.E, & Mouneimne G. (2021). Breast tumor stiffness instructs bone metastasis via maintenance of mechanical conditioning. Cell reports, 35(13), 109293.
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