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Plan apo vc 60x 1.4 na oil immersion objective lens

Manufactured by Nikon
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Sourced in Belgium

The Plan Apo VC 60x 1.4 NA oil immersion objective lens is a high-magnification, high-numerical aperture objective designed for use in advanced microscopy applications. It features a 60x magnification and a numerical aperture of 1.4, making it suitable for a variety of microscopy techniques that require high-resolution imaging.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

Close spelling variants of this product

VC Plan Apo 60X/1.4 NA oil objective Plan Apo VC 60X/ 1.4 Oil objective Plan Apo VC 60x/1.4 objective Plan Apo VC 60x lens oil Plan Apo 1.4 NA oil objective 60x/1.40 NA Plan Apo oil immersion objective PLAN APO 60X/1.4 NA Apo 60x 1.4 NA oil Plan Apo VC/1.4 NA Plan Apo VC objective lens

2 protocols using plan apo vc 60x 1.4 na oil immersion objective lens

1

Labeling CD8+ T Cells with Liposomes

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DiD-labeled liposomes were conjugated to the cell surface of CD8 + T cells as previously described, followed by a cell nucleus stain with 10 µg ml -1 Hoechst 33342 (Molecular Probes) and a CD8-AlexaFluor488 antibody stain (BD Pharmingen). The dyes were respectively excited by a 636 nm diode laser, a 408 nm diode laser, and a 488 nm argon-ion laser (all purchased from CVI Melles Griot). Images were recorded with a Plan Apo VC 60x 1.4 NA oil immersion objective lens (Nikon).
Wayteck L., Dewitte H., De Backer L., Breckpot K., Demeester J., De Smedt S.C, & Raemdonck K. (2016). Hitchhiking nanoparticles: Reversible coupling of lipid-based nanoparticles to cytotoxic T lymphocytes. Biomaterials, 77.
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2

Cy5-Labeling of CD8+ T Cells

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CD8 + T cells were incubated for 15 min with 100 µg ml -1 Cy5-labeled maleimide (GE Healthcare Life Sciences, Diegem, Belgium) at room temperature. After washing the cells with PBS, the cells were fixed with 4% paraformaldehyde (Sigma-Aldrich, Bornem, Belgium). Confocal microscopy images were acquired using a Nikon C1si confocal laser scanning microscope (Nikon Benelux, Brussels, Belgium) equipped with a Plan Apo VC 60x 1.4 NA oil immersion objective lens (Nikon). The Cy5 dye was excited with a 636 nm diode laser (CVI Melles Griot, NM, USA).
Wayteck L., Dewitte H., De Backer L., Breckpot K., Demeester J., De Smedt S.C, & Raemdonck K. (2016). Hitchhiking nanoparticles: Reversible coupling of lipid-based nanoparticles to cytotoxic T lymphocytes. Biomaterials, 77.
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