Short tandem repeat dna profiling

MPNST cell lines S462, S462TY, and ST8814 were kindly granted by Prof. Vincent Keng and Prof. Jilong Yang. MPNST cell lines were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in a 37°C, 5% CO₂ incubator. All the cell lines were tested mycoplasma negative every 3 months. Verification of cell lines was confirmed by Short Tandem Repeat DNA profiling (Applied Biological Materials Inc., Canada).
Reagents and antibodies used in this article are described in Supplementary Table S1.

One HSC line and two MPNST cell lines (sNF02.2, sNF96.2) were purchased from ATCC (American Type Culture Collection, ATCC). Five MPNST cell lines (ST88-14, STS26T, T265, S462, and S462TY) were kindly granted by Prof. Vincent Keng (Li X.X. et al., 2018 (link)) and Prof. Jilong Yang (Du et al., 2013 (link)). All MPNST cell lines were derived from NF1 patients, except STS26T. Cell lines were maintained in DMEM, 10% FBS and penicillin/streptomycin (Gibco, United States) and were tested mycoplasma negative every 3 months. Verification of cell lines was confirmed by Short Tandem Repeat DNA profiling (Applied Biological Materials Inc., Canada).

Two MPNST cell lines (STS26T and S462) were generously donated by Dr Vincent Keng. S462 was derived from an NF1-MPNST patient, whereas STS26T was derived from a sporadic MPNST patient. The two MPNST cell lines were maintained in high-glucose DMEM with 10% FBS and 1% penicillin/streptomycin in a 37°C, 5% CO₂ incubator providing a humidified atmosphere. The culture medium was replaced every 3 days, and the cells were passaged using 0.25% trypsin until they approached 80% confluence. All cell lines were tested mycoplasma negative every 3 months. Verification of cell lines was confirmed by Short Tandem Repeat DNA profiling (Applied Biological Materials Inc, Canada).