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4 protocols using sulfuric acid

1

Tung Oil-Based Polymer Synthesis

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Glycidyl methacrylate (GMA) was provided by J&K Scientifific Ltd., Beijing, China. Ammonium chloride (NH4Cl), potassium persulfate (KPS), sulfuric acid (H2SO4, 98%), hydrochloric acid (HCl, 37%), sodium hydroxide (NaOH), sodium chloride (NaCl), phenol, formaldehyde (37%), and resorcinol, were all provided by Tianjin Kemiou Chemical Reagent Co., Ltd., Tianjin, China. Tung oil (410 mPa s, 25 °C) was obtained from Guangzhou Hanhao Chemical Co. Ltd., Guangzhou, China. Trimethylolpropane tris (3-mercaptopropionate) (TMMP), trimethylolpropane triglycidyl ether (TMTGE), and 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD, 98%) were all purchased from Sigma Aldrich Co. Ltd., Shanghai, China. All reagents were used without further purification.
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2

Synthesis and Characterization of Gold Nanoparticles

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All chemicals and solvents were commercially available and used without further purification. Tetrachloroauric(III) acid trihydrate (99% metal basis) was purchased from Aladdin Co., Ltd. Sodium borohydride (99.8%) was purchased from Sigma-Aldrich. Triphenylphosphine (>99%), 2,4-dimethylbenzenethiol (98%) were purchased from Saan Chemical Technology Co., Ltd. Methanol (>99%), dichloromethane (>99%), toluene (>99%), hexanes (>99%), and sulfuric acid (98%) were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd.
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3

Spectrophotometric Determination of Chromium

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Potassium dichromate (K2Cr2O7), ammonium metavanadate (NH4VO3), ammonium chloride (NH4Cl), sulfuric acid (H2SO4), diphenylcarbazide (C13H14N4O), magnesium chloride (MgCl2), sodium hydroxide (NaOH), sodium phosphate (Na3PO4), acetone (CH3COCH3), Nessler's reagent and sodium potassium tartrate tetrahydrate (KNaC4H4O6·4H2O) were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd.
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4

Microalgal Lipid Extraction and Characterization

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Chromatographic grade n-hexane and methanol were purchased from J&K Scientific Ltd (China). Sulfuric acid (purity 95–98 %) was analytical grade and was obtained from Tianjin Kemiou Chemical Reagent Co., Ltd (China). Chemically pure glyceryl trioleate (TAG) was purchased from Sinopharm Chemical Reagent Co., Ltd (SCRC) China. Methyl hexadecanoate was purchased from Sigma-Aldrich (USA). Soybean oil was purchased from a local supermarket, and the microalgal lipids were prepared using I. zhangjiangensis and Chaetoceros sp. cultured in our lab. Chlorella Vulgaris and Nannochloropsis were obtained from South China University of Technology and Beihang University, respectively.
Syringe pumps were purchased from Baoding Longer Precision Pump Co., Ltd. The Y-shaped MR is a simple triple valves with a 0.25 mm inner diameter from Dalian Elite Analytical Instruments Co., Ltd. The T-shaped and Q-shaped with 0.25 mm inner diameter MRs used in the studies were manufactured using glasses.
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