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36ga nanofil needle

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The 36ga NanoFil needle is a precision laboratory instrument designed for delicate and accurate fluid handling tasks. It features a thin, 36-gauge needle tip for minimal sample disruption. The NanoFil needle is constructed with durable materials to ensure reliable performance in a variety of lab applications.

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3 protocols using 36ga nanofil needle

1

Sciatic Nerve Injury Model in Rats

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Unilateral or bilateral sciatic nerve injection was performed on adult female Fischer 344 rats (120-135g) unresponsive to toe or tail pinch under isoflurane anaesthetic. The sciatic nerve was exposed as above and injected with 4μl (2μl/branch) 1%wt/vol LPC in PBS with 10%vol/vol DMSO, 2μl (1μl/branch) of 0.01%, 0.05% or 0.1%wt/vol EtBr in PBS or PBS alone. Injections were made longitudinally towards the DRG, with a 36ga NanoFil needle (World Precision Instruments Inc., Sarasota, FL) at a rate of 2μl/min and the needle was held in place for an additional 10s following injection. After injection, the skin was closed with surgical staples.
Hollis ER I.I., Ishiko N., Tolentino K., Doherty E., Rodriguez M.J., Calcutt N.A, & Zou Y. (2014). A novel and robust conditioning lesion induced by ethidium bromide. Experimental neurology, 265, 30-39.
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2

Cre-Expressing AAV2/6 Injection in Mouse Motor Cortex

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Adult female C57BL/6J mice (6.1±0.1 weeks old) were deeply anaesthetized with isoflurane until unresponsive to toe and tail pinch and the area over the skull was shaved and cleaned with povidone-iodine before incision. The skull was thinned bilaterally over the motor cortex and self-complementary AAV2/6 Cre-HA (Salk Institute for Biological Studies Gene Transfer, Targeting and Therapeutics Core, La Jolla, CA) [1.49×1011 genome copies/ml] was injected into 10 sites per hemisphere (250nl/site) with a 36ga NanoFil needle (World Precision Instruments Inc., Sarasota, FL).
Hollis ER I.I., Ishiko N., Yu T., Lu C.C., Haimovich A., Tolentino K., Richman A., Tury A., Wang S.H., Pessian M., Jo E., Kolodkin A, & Zou Y. (2016). Ryk controls remapping of motor cortex during functional recovery after spinal cord injury. Nature neuroscience, 19(5), 697-705.
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3

Cre-Expressing AAV2/6 Injection in Mouse Motor Cortex

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Adult female C57BL/6J mice (6.1±0.1 weeks old) were deeply anaesthetized with isoflurane until unresponsive to toe and tail pinch and the area over the skull was shaved and cleaned with povidone-iodine before incision. The skull was thinned bilaterally over the motor cortex and self-complementary AAV2/6 Cre-HA (Salk Institute for Biological Studies Gene Transfer, Targeting and Therapeutics Core, La Jolla, CA) [1.49×1011 genome copies/ml] was injected into 10 sites per hemisphere (250nl/site) with a 36ga NanoFil needle (World Precision Instruments Inc., Sarasota, FL).
Hollis ER I.I., Ishiko N., Yu T., Lu C.C., Haimovich A., Tolentino K., Richman A., Tury A., Wang S.H., Pessian M., Jo E., Kolodkin A, & Zou Y. (2016). Ryk controls remapping of motor cortex during functional recovery after spinal cord injury. Nature neuroscience, 19(5), 697-705.
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