Sk es 1

Manufactured by Corning

Sourced in United States

The SK-ES-1 is a laboratory instrument designed for electrochemical analysis. It provides precise measurements of electrochemical properties, such as potential, current, and impedance. The SK-ES-1 is a versatile tool that can be used in a variety of research and industrial applications.

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Lab products found in correlation

2 protocols using sk es 1

Cell Line Cultivation for Cancer Research

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The Ewing sarcoma (A-673, SK-ES-1), rhabdomyosarcoma (SJCRH30), medulloblastoma (D283, D341, DAOY), and breast cancer (HCC1806) cell lines were purchased from the American Type Culture Collection, (Manassas, VA, USA). The hepatoblastoma cell line (Hep293TT) was obtained directly from Dr. Gail Tomlinson.^{23 (link)} A-673, SK-ES-1, SJCRH30, HCC1806, and Hep293TT cells were grown in RPMI-1640 medium (Corning, Corning, NY, USA) with 5% fetal bovine serum (Corning) and 50 μg/mL gentamicin (Gibco/Thermo Fisher Scientific, Waltham, MA, USA). The D283, D341, and DAOY cells were grown in IMEM (Gibco) with 5% fetal bovine serum and 25 μg/mL gentamicin. Cells were grown at 37 °C with 5% CO₂ in humidified incubators. The cells were used within four months of retrieval from liquid nitrogen. The A-673, SJCRH30, and D283 cells lines were validated by STR profiling (Genetica DNA Laboratories, Burlington, NC, USA).

Kil Y.S., Risinger A.L., Petersen C.L., Liang H., Grkovic T., O’Keefe B.R., Mooberry S.L, & Cichewicz R.H. (2020). Using the Cancer Dependency Map to Identify the Mechanism of Action of a Cytotoxic Alkenyl Derivative from the Fruit of Choerospondias axillaris. Journal of natural products, 83(3), 584-592.

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Cultivation of Ewing Sarcoma and Control Cell Lines

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The Ewing sarcoma cell lines that were used were A673, SK-ES-1 and RD-ES (ATCC). As a control non-Ewing sarcoma cancer cell line, the osteosarcoma cell line U2OS was used. A673 cells were cultured in Dulbecco's modified Eagle's medium (D MEM; Corning. Mediatech Inc.) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic (Gibco Life Technologies). U2OS cells were cultured in McCoy's 5A (Corning. Mediatech Inc.) supplemented with 10% FBS and 1% antibiotic-antimycotic (Gibco Life Technologies). SK-ES-1 cells were cultured in McCoy's 5A medium (Corning. Mediatech Inc.) supplemented with 15% FBS and 1% antibiotic-antimycotic (Gibco Life Technologies). RD-ES cells were cultured in RPMI-1640 (Corning. Mediatech Inc.) supplemented with 15% FBS and 1% antibiotic-antimycotic (Gibco Life Technologies). BJ cells were cultured in Minimum Essential Media (MEM; Corning. Mediatech Inc.) supplemented with 10% FBS and 1% antibiotic-antimycotic (Gibco Life Technologies). All cell lines were grown at 37°C and 5% CO2.

Martin J.C., Gupta A., Hagoel T.J., Gao L., Lynch M.L., Woloszynska‐Read A., Melendy T., Kane J.F., Kuechle J., & Ohm J.E. (2021). DDK inhibition disrupts replication leading to mitotic catastrophe in Ewing sarcoma.

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