Antibodies used were anti-human CD3ε (OKT3, American Type Culture collection, Manassas, VA), anti-CD28 (clone CD28.2, BD Biosciences), and anti-TSAd-DyLight 488 (clone OTI3C7, Origene), anti–IL-4 (11B11, Bio X Cell), anti-IFNγ-FITC, anti-CD4-PerCP-Cy5.5 (RM4–5) and anti-CD44-FITC (KM201, BD), anti-CD62L-PE (MEL-14, Southern Biotech), anti-γ–tubulin (GTU-88, Sigma) and anti- Id-specific TCR-PE (GB11354 (link)). Polyclonal antibodies were anti-PAR3 (Cat# 07-330, Millipore), anti-PKCξ (Η - 1), anti-PKCθ (C-18), anti-Scrib (C-6) and anti-SAP97 (H-60, Santa Cruz). Secondary antibodies were goat anti-donkey, goat anti-rabbit or isotype specific anti-mouse conjugated to Alexa Fluor 488 (Thermo Fisher Scientific). Cytkines were IL-2, IL-12 (Peprotech). Fluorescent markers were phalloidin Alexa Fluor 647, cell tracker violet (CTV), SNARF, LIVE/DEAD Fixable Near-IR (all from Thermo Fisher).
Anti human cd3ε okt3
Manufactured by American Type Culture Collection
Anti-human CD3ε (OKT3) is a monoclonal antibody that binds to the CD3 epsilon chain, a component of the T cell receptor complex. It is used in research applications to detect and study T cells.
Automatically generated - may contain errors
Lab products found in correlation
Cell tracker violet, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647, by Thermo Fisher Scientific (1 mentions)
Anti cd4 percp cy5, by BD (1 mentions)
Anti ifn γ fitc, by BD (1 mentions)
Anti γ tubulin gtu 88, by Merck Group (1 mentions)
Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions)
Live dead fixable near ir, by Thermo Fisher Scientific (1 mentions)
Snarf, by Thermo Fisher Scientific (1 mentions)
Anti par3, by Merck Group (1 mentions)
Anti cd28 clone cd28, by BD (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Anti il 4 11b11, by BioXCell (1 mentions)
Horseradish peroxidase hrp conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Anti nck, by Merck Group (1 mentions)
2 protocols using anti human cd3ε okt3
1
Antibody-based Characterization of T Cell Activation
2
Characterization of T Cell Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following monoclonal antibodies were used: anti-Lck (3A5), anti-SLP-76 (F7), and anti-GST (B12) (Santa Cruz Biotechnology,Inc.), anti-HA (HA.11, Bio Site), anti-Nck (108; BD Transduction Laboratories™), anti-human CD3ε (OKT3; American Type Culture Collection), anti-phosphotyrosine (4G10; Upstate Biotechnology), and unconjugated and DyLight 488 conjugated anti-TSAd (anti-SH2D2A) (3C7, Origene). The following polyclonal antibodies were used: anti-Nck (Millipore), anti-Vav (C-14; Santa Cruz Biotechnology,Inc.) and anti-TSAd (1715 T, provided by V. Shapiro). Horse radish peroxidase (HRP)-conjugated goat anti-mouse IgG, goat anti-mouse IgG light chain specific and goat anti-rabbit IgG (all from Jackson ImmunoResearch Laboratories), and goat-anti rabbit IgG Alexa Fluor® 647 conjugate (Molecular Probes) were used as secondary antibodies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!