H1563

Manufactured by American Type Culture Collection

Sourced in United States

H1563 is a laboratory instrument designed for the cultivation and maintenance of various cell and tissue cultures. It provides a controlled environment for the growth and propagation of biological samples.

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Close spelling variants of this product

NCI-H1563

7 protocols using h1563

Cell Line Authentication and Culturing

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BEAS2B (CRL-9609), H1563 (CRL-5875), A549 (CCL-185), H23 (CRL-5800), H358 (CRL-5807), PC3 (CRL-1435), U2OS (HTB-96), and HeLa (CCL-2) were obtained from ATCC. Cell lines were authenticated by short tandem repeat profiling and Mycoplasma testing via PCR (Genetica Corp) in August 2018 (A549, H23,H358,H1563) or in October 2019 (U2OS). PC3 and HeLa cells were used within four passages following thaw from original ATCC purchased stocks. All cell culture reagents were purchased from Gibco/Life Technologies Inc., except for the FBS used to supplement the media, which was obtained from Hyclone. Cell lines were cultured in RPMI-1640 base medium (11875) with the exception of U2OS, which were cultured in DMEM (11965), supplemented with 10% FBS and 100 U/mL penicillin/streptomycin (15140122). Cells were cultured at 37°C in a humidified incubator at 21% oxygen or 5% oxygen, 5% CO₂ (HeraCell Tri-Gas, Thermo Fisher Scientific, Inc.).

Samaranayake G.J., Troccoli C.I., Zhang L., Huynh M., Jayaraj C.J., Ji D., McPherson L., Onishi Y., Nguyen D.M., Robbins D.J., Karbaschi M., Cooke M.S., Barrientos A., Kool E.T, & Rai P. (2019). The Existence of MTH1-independent 8-oxodGTPase Activity in Cancer Cells as a Compensatory Mechanism against On-target Effects of MTH1 Inhibitors. Molecular cancer therapeutics, 19(2), 432-446.

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Cell Line Characterization and Culture

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A549 (ATCC: CCL-185), H1299 (ATCC: CRL-5803), and H1563 (ATCC: CRL-5875) NSCLC cell lines were purchased directly from ATCC. All cell lines were initially expanded and low passage numbers aliquots were frozen back to ensure experiments were conducted in cell lines with similar passage numbers. Cell lines were screened for mycoplasma at regular intervals, including when cell lines were frozen back. All cells were grown in RPMI 1640 (Lonza, Basel, Switzerland: 12-167F) with 10% Fetal Bovine Serum (Sigma-Aldrich, St. Louis MO, USA: F0926), Penicillin/Streptomycin (Gibco, Waltham, MA, USA: 15140-122), and 2 mM Glutamax (Gibco: 35050-061) and were maintained in a 37 °C humidified incubator with 5% CO₂. Purified artesunate (HY-N0193) was purchased from MedChem Express (Monmouth Junction, NJ, USA) and ML385 (SML1833) was purchased from Sigma-Aldrich.

Hill K.S., McDowell A., McCorkle J.R., Schuler E., Ellingson S.R., Plattner R, & Kolesar J.M. (2021). KEAP1 Is Required for Artesunate Anticancer Activity in Non-Small-Cell Lung Cancer. Cancers, 13(8), 1885.

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Cell Culture Protocols for Lung Cell Lines

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BEAS2B, A549, H460, H23, H358 and H1563 cells were obtained from the
American Type Culture Collection (ATCC). All cells were grown at 37°C in
21% oxygen or 5% oxygen where indicated and 5% CO₂. BEAS2B cells were
maintained in DMEM:F12 complete base media supplemented with 10% fetal bovine
serum (FBS) and 100 units/ml penicillin/streptomycin. A549, H23, H358 and H1563
cells were maintained in RPMI-1640 complete base media supplemented with 10% FBS
and 100 units/ml penicillin/streptomycin (Gibco, Life Technologies).

Patel A., Burton D.G., Halvorsen K., Balkan W., Reiner T., Perez-Stable C., Cohen A., Munoz A., Giribaldi M.G., Singh S., Robbins D.J., Nguyen D.M, & Rai P. (2014). MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant pathways. Oncogene, 34(20), 2586-2596.

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Cell Culture Protocols for Lung Cell Lines

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Lung Cancer Cell Line Culture Protocols

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Lung cancer cell lines H661, H1299, H1437, H1563, and H1975 were obtained from the American Type Culture Collection (ATCC). All lung cancer cells were maintained at 37 °C in 5% CO2 in RPMI Medium 1640 (Gibco, Gaithersburg, MD, USA) supplemented to contain 10% fetal bovine serum (FBS) and 2mM l-glutamine. L929 cells were maintained in spinner culture at 37 °C in Joklik’s MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented to contain 5% FBS, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 25 ng/mL amphotericin B (Sigma-Aldrich).

Simon E.J., Howells M.A., Stuart J.D, & Boehme K.W. (2017). Serotype-Specific Killing of Large Cell Carcinoma Cells by Reovirus. Viruses, 9(6), 140.

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Culturing BEAS2B and NSCLC Cell Lines

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The human bronchial epithelial cell line BEAS2B and NSCLC cell lines A549, H460, H1965, H2228, H1563, and H661 cells were all purchased from the American Type Culture Collection (Rockville, MD, USA) and PC9 NSCLC cells were obtained from Cell Bank of Chinese Academy of Medical Sciences (Beijing, China). These cells were all maintained in DMEM medium (Hyclone) supplemented with 10% fetal bovine serum (FBS; Hyclone), 100 units/l penicillin, and 100 μg/l streptomycin (Life Technologies). The BEAS2B and PC9 cells stably expressing control or Mer were cultured in similar medium additionally supplemented with 1 μg/ml puromycin.

Xie S., Li Y., Li X., Wang L., Yang N., Wang Y, & Wei H. (2015). Mer receptor tyrosine kinase is frequently overexpressed in human non-small cell lung cancer, confirming resistance to erlotinib. Oncotarget, 6(11), 9206-9219.

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NSCLC Cell Line Collection and Maintenance

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Eight NSCLC human cell lines (A549, H1299, H1563, H1437, H661, H2126, H1573, and H1975) were obtained from the American Type Culture Collection (ATCC, Rockville, MD). A normal human bronchial cell line, HBEC3-KT, was a gift from John Minna (UT-Southwestern). Cells were maintained in complete media with 10% FBS, antibiotics/antimycotics and not passed continuously more than 4 weeks. All cell lines were originally authenticated by ATCC.

Lewis K.M., Bharadwaj U., Eckols T.K., Kolosov M., Kasembeli M.M., Fridley C., Siller R, & Tweardy D.J. (2015). SMALL-MOLECULE TARGETING OF SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION (STAT) 3 TO TREAT NON-SMALL CELL LUNG CANCER. Lung cancer (Amsterdam, Netherlands), 90(2), 182-190.

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