Cleaved parp antibody

The following antibodies were used in this study: Cleaved caspase-3, β-actin antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA). Cleaved PARP antibody (Abcam, Inc., Cambridge, UK). Na/K ATPase, PEDF, ATGL (PEDF-R), phosphorylated Akt (Ser473) and total Akt antibodies (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Hoechst 33342 and Dexamethasone (DEX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). DEX was dissolved in ethanol and stored at −20 °C. Cell counting kit (CCK-8) was purchased from Dojindo (Kumamoto, Japan). Mem-PER™ Plus Kit (Membrane Protein Extraction Kit) was obtained from Biovision, Inc. (San Francisco, CA, USA). Protein A/G-agarose used in this study was obtained from Thermo Scientific (Waltham, MA, USA). Annexin-V-FLUOS Staining Kit (Roche, Branford, CT, USA) was used to assess apoptosis. ELISA kits for PEDF and lysophosphatidic acid (LPA) were purchased from Cloud-clone Corp. (Houston, TX, USA).

Carboplatin and olaparib were purchased from Selleck Chemicals (Houston, TX, USA). Thiostrepton was obtained from Sigma-Aldrich. Pan-cytokeratin antibody was acquired from Bio Genex Laboratories (San Ramon, CA, USA), Ki67-antibody from DCS (Hamburg, Germany), cleaved-PARP-antibody from Abcam (Cambridge, UK) and FOXM1-antibody from Cell Signaling Technology (Danvers, MA, USA). Antibody against CD3 was purchased from Bio-Rad Laboratories, Inc. (Hercules, CA, USA), antibody against CD8 from Cell Signaling Technology and antibody against CD68 from Agilent. Secondary antibodies for immunofluorescence Alexa Fluor 568 goat anti-mouse antibody and Alexa Fluor 647 goat anti-rabbit antibody were obtained from Invitrogen (Carlsbad, CA, USA). Secondary biotinylated goat anti-rat antibody was purchased from Vector Laboratories (Burlingame, CA, USA) and goat anti-mouse antibody was acquired from Sigma Aldrich for immunohistochemical analysis.