For SPECT imaging and fluorescence detection concentrated NP were formulated with HC-PGC that carried covalently linked residues of both Alexa Fluor488 (AF488) and hydroxyphenylpropionate (at approximately 1% of total available amino groups of PLL). Hydroxyphenylpropionate residues were labeled with a gamma-emitting I-123 isotope (half-Life: 13.2 h, Na[123I], Nordion) in the presence of iodogen-coated tubes (Pierce™ Iodination Tubes, Thermo Fisher) according to the manufacturer’s instructions. Briefly, 0.1 mg of a mixture of AF488 and hydroxyphenylpropionate- labeled M5P10OL and M5P10ST (1:1 by weight) containing a mixture of EFV and ELV were combined with 2 mCi of Na123I dissolved in 25mM Tris hydrochloride, 0.4M NaCl pH 7.5, the reaction was quenched by removing labeled NP from the tube and adding 0.01 mM L-tyrosine followed by 0.1 mM sodium iodide to displace free [123I]iodide. The resultant dual-labeled HC-PGC NP were purified by using Biogel P-30 column blocked with 0.01% Tween-20 and equilibrated with sterile HBS. Radioiodination labeling efficiency was approximately 50% as determined by using iTLC-SG paper plates (Agilent).
Pierce iodination tubes
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Pierce™ Iodination Tubes are laboratory equipment designed for the iodination of proteins. They provide a convenient and controlled environment for the radioactive labeling of proteins using iodine isotopes.
Automatically generated - may contain errors
Lab products found in correlation
Na123i, by Nordion (1 mentions)
Nanospect ct, by Bioscan (1 mentions)
Pierce iodination reagent, by Thermo Fisher Scientific (1 mentions)
Hrp linked anti rabbit igg, by GE Healthcare (1 mentions)
Rabbit anti human gapdh, by Santa Cruz Biotechnology (1 mentions)
Fluorescently labeled secondary antibody, by Jackson ImmunoResearch (1 mentions)
Pre cast 4 15 polyacrylamide gels, by Bio-Rad (1 mentions)
125iodine 125i, by PerkinElmer (1 mentions)
Pvdf membrane, by Pall Corporation (1 mentions)
Mouse igg, by Jackson ImmunoResearch (1 mentions)
Polystyrene beads, by Polysciences (1 mentions)
4 protocols using pierce iodination tubes
1
Dual-Labeled HC-PGC Nanoparticles for SPECT and Fluorescence
2
SPECT Imaging of Amoxicillin-Loaded Nanoparticles
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Pathogen-free BALB/c mice (4 weeks old) were purchased from the National Laboratory Animal Center (Taipei, Taiwan). All experimental mice received care on the basis of the guidelines outlined in the Guide for the Care and Use of Laboratory Animals (8th edition). The in vivo experiments were conducted using protocols approved by the National Taiwan University College of Medicine and College of Public Health Institutional Animal Care and Use Committee (Approved NO. 20190127).
For the single-photon emission computed tomography (SPECT) study, amoxicillin was first radiolabeled with 123iodine (123I, emitting 159 KeV photons) using an idoge-tube (Pierce Iodination Tubes, Thermo Fisher Scientific, Rackford, IL, USA) [30 (link)] and then loaded into the prepared CAANs (123I-CAANs). BALB/c mice were fed with the 123I-amoxicillin or 123I-CAANs, and then SPECT/CT images were obtained using a NanoSPECT/CT (Bioscan Inc., Poway, CA, USA) at 4 and 24 h post oral administration. The mice were euthanized 24 h post oral administration, and their gastrointestinal tracts were removed. SPECT/CT images of the gastrointestinal tracts were then obtained.
For the single-photon emission computed tomography (SPECT) study, amoxicillin was first radiolabeled with 123iodine (123I, emitting 159 KeV photons) using an idoge-tube (Pierce Iodination Tubes, Thermo Fisher Scientific, Rackford, IL, USA) [30 (link)] and then loaded into the prepared CAANs (123I-CAANs). BALB/c mice were fed with the 123I-amoxicillin or 123I-CAANs, and then SPECT/CT images were obtained using a NanoSPECT/CT (Bioscan Inc., Poway, CA, USA) at 4 and 24 h post oral administration. The mice were euthanized 24 h post oral administration, and their gastrointestinal tracts were removed. SPECT/CT images of the gastrointestinal tracts were then obtained.
3
Radiolabeling of Hyaluronic Acid
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HA10 was purchased from Lifecore Biomedical (Chaska, Minnesota). Highly purified HA35 fragment preparations were obtained from Shaoxing HH Technology, Inc. (Shaoxing, China). Cyanogen bromide (CNBr), DL-tyrosine (Tyr), tyramine (TA), sodium glucoheptonate (GH), stannous chloride dihydrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), LPS from Escherichia coli 0127:B8 and other chemicals were purchased from Millipore Sigma (St. Louis, MO). 125I was obtained from PerkinElmer (Boston, MA). 99mTc pertechnetate (99mTcO4−) was provided by Cardinal Health (Tucson, AZ). Pierce Iodination tubes pre-coated at the bottom with 50 μg Pierce Iodination Reagent were obtained from Thermo Fisher Scientific™ (Waltham, MA).
4
Antibody and Reagent Sources
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