Cpi203

I-BET726 was purchased from Adooq (Shanghai, China). MTT, AZD5153, PD98059 and LY294002, JQ1 and CPI203 were purchased from Sigma (Shanghai, China). Z-DEVD-fmk, Z-LEHD-fmk, and Z-VAD-fmk were provided by Calbiochem (La Jolla, CA). Antibodies for c-Myc (#9402), Cyclin D1 (#2922), BRD4 (#13440), Bcl-2 (#15707), SphK1 (#12071), phosphorylated (“p”)-Akt (Ser-473) (#9271), Akt1/2 (#9272), p-p44/42 MAPK (Erk1/2) (#9101) and Erk1/2 (#9102), cleaved-caspase-3 (#9664), cleaved-caspase-8 (#9496), cleaved-caspase-9 (#20750), cleaved-poly (ADP-ribose) polymerase (PARP) (#5625), β-tubulin (#15115) and β-actin (#3700) were purchased from Cell Signaling Tech (Beverly, MA).

VS-5584, JQ1, and CPI203 were obtained from Sigma-Aldrich (St. Louis, MO). Cell culture reagents were purchased from Gibco (Grand Island, NY). The antibodies were purchased from Cell Signaling Technology (Danvers, MA). Puromycin was obtained from Sigma-Aldrich.

A1874 was obtained from Hanxiang BioTech (Shanghai, China). Puromycin, JQ1, CPI203, I-BET151, N-acetyl-cysteine (NAC) and pifithrin-α, polybrene and CCK-8 were purchased from Sigma-Aldrich (St. Louis, MO). All cell culture reagents were provided by Hyclone Co. (Logan, UT). Antibodies for c-Myc (#9402), Cyclin D1 (#2922), BRD4 (#13440), Bcl-2 (#15707), Erk1/2 (#9102), p53 (#9282), cleaved-caspase-3 (#9664), cleaved-caspase-9 (#20750), cleaved-poly (ADP-ribose) polymerase (PARP) (#5625), Bcl-2 (#15707) and β-tubulin (#15115) were purchased from Cell Signaling Tech (Beverly, MA). Caspase inhibitors, z-VAD-fmk and z-DEVD-fmk, were provided by Thermo-Fisher (Shanghai, China). Lipofectamine 2000, TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling), Annexin V and propidium iodide (PI) were purchased from Thermo-Fisher Invitrogen (Carlsbad, CA). The BrdU ELISA kit was provided by Roche Diagnostics (Basel, Switzerland).