Ab48871

Manufactured by Abcam

Sourced in United States

Ab48871 is a rabbit polyclonal antibody targeting Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). GAPDH is a key enzyme involved in glycolysis. This antibody can be used for the detection of GAPDH in various applications.

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Lab products found in correlation

4 protocols using ab48871

Comprehensive Antibody Characterization Panel

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The antibodies to Tet1 (ab191698), Tet2 (ab94580), P2rX7 (ab48871), CD146 (ab24577), PDGFRα (ab65258), and OCN (ab10911) were purchased from Abcam (Cambridge, MA, USA). Antibodies to ALP (sc-28904), CD9 (sc-9148), and CD81 (sc-9158) were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Antibody to Runx2 (8486) was obtained from Cell Signaling Technology (Danvers, MA, USA). Antibody to Tet3 (20602) was purchased from Novus Biologicals (Littleton, CO, USA). Anti-CD34-PE (551387) and SCA1-PE (553108) antibodies were purchased from BD Biosciences (San Jose, CA, USA). Anti-CD105-PE (12-1051-82), CD45-PE (25-0454-82), CD73-PE (12-0739-42), and CD90-PE (15-0902-82) antibodies were purchased from eBioscience (San Diego, CA, USA). Anti-β-Actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Yang R., Yu T., Kou X., Gao X., Chen C., Liu D., Zhou Y, & Shi S. (2018). Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter. Nature Communications, 9, 2143.

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Antibody Panel for Extracellular Vesicle Analysis

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The antibody to ALPL (11187-1-AP) was purchased from Proteintech (Wuhan, China). Antibody to CD81 (GTX31381) was purchased from GeneTex (Texas, USA). Antibodies to CD9 (ab92726), TSG101 (ab125011), PDGFBB (ab16829) and P2X7 (ab48871) were purchased from Abcam (Cambridge, USA). Antibodies to VEGF (SC-57496) and Endostatin (SC-32720) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibody to Angiostatin (AF-226) was purchased from R&D (R&D sysstems, USA). Anti β-Tubulin antibody was purchased from Cwbiobiotech (Beijing, China).

Dong J., Zhao W., Zhao J., Chen J., Liu P., Zheng X., Li D., Xue Y, & Zhou H. (2024). ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion. Journal of Nanobiotechnology, 22, 172.

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Betulin Modulation of Inflammatory Pathways

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Betulin (BT) (BET201102212) was purchased from Skyherb Technologies (Hangzhou, China). Primary antibodies of SREBP1 (ab3259), lipin1 (ab181389), lipin2 (ab176347), P2X7r (ab48871), NLRP3 (ab4207), FANS (ab22759), PPARγ (ab19481) and GAPDH (ab8245) were purchased from Abcam (Cambridge, MA, USA). Primary antibodies of PPARα (sc-9000), PGC-1α (sc-518025), IL-6 (sc-28343), IL-18 (sc-133127), IL-1α (sc-393998) and caspase-1 (sc-514) were obtained from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Primary antibody of IL-1β (AF-401-NA) was purchased from R&D. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit (ab97051) and rabbit anti-mouse (ab6728) were purchased from Abcam. Horseradish peroxidase (HRP)-conjugated rabbit anti-goat (HAF017) was purchased from R&D. The BCA Protein Assay Kit was obtained from Beyotime (Jiangsu, China). The Mouse IL-1β Uncoated ELISA Kit was purchased from Invitrogen (Carlsbad, CA, USA) and mouse IL-6 ELISA Kit was obtained from NeoBioscience (EMC004; Shenzhen, China). DMSO was purchased from Sigma Chemical Co (St. Louis, MO, USA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle medium (DMEM) basic (1X) were purchased from Gibco (MA, USA). All other chemical reagents were analytical grade.

Dou J.Y., Jiang Y.C., Hu Z.H., Yao K.C., Yuan M.H., Bao X.X., Zhou M.J., Liu Y., Li Z.X., Lian L.H., Nan J.X, & Wu Y.L. (2021). Betulin Targets Lipin1/2-Meidated P2X7 Receptor as a Therapeutic Approach to Attenuate Lipid Accumulation and Metaflammation. Biomolecules & Therapeutics, 30(3), 246-256.

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Quantifying P2X7R and CD8+ TILs

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For immunohistochemical examination, 5-µm thick sections from paraffinized tumor tissues were used. In the immunophenotypic staining process, the following antibodies were applied: Anti-P2X7R (ab48871, ABCAM, Cambridge CB2 0AX, UK) and anti-CD8 (SP57, Ventana, Arizona, USA). The prepared paraffinized sections were stained using the Ventana BenchMark ULTRA coater (Ventana, Tucson, AZ-85755, USA) and the ultraView Universal DAB kit (Ventana, Tucson, AZ-85755, USA) following the manufacturer’s instructions. The intensity and distribution of P2X7R expression in cancer cells were evaluated immunohistochemically. In addition, the TILs density was assessed by CD8 staining. Immunostaining of each sample was examined by two objective investigators unaware of the clinical features, survival data, and pathological features of the patients.

Calik I., Calik M., Sarikaya B., Ozercan I.H., Arslan R., Artas G, & Dagli A.F. (2020). P2X7 receptor as an independent prognostic indicator in gastric cancer. Bosnian Journal of Basic Medical Sciences, 20(2), 188-196.

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