The following antibodies were used for immunoblotting: anti-SCD1 (1:1,000, cat. no. 2438S, Cell Signaling Technology), anti-ABCA1 (1:1,000; Lee et al., 2005 (link)), and anti-actin (1:5,000, cat. no. sc-47778, Santa Cruz Biotechnology). Appropriate HRP-conjugated secondary antibodies for immunoblotting were purchased from Dako and Cell Signaling Technology. The following antibodies were used for flow cytometry: anti-SCD1 (1:200, cat. no. ab19862, Abcam) and anti-ABCA1 (1:400, cat. no. NB400-105, Novus Biologicals). The following antibodies were used for immunofluorescence: anti-SCD1 (1:250, cat. no. ab19862, Abcam), anti-CCR7 (1:100, cat. no. ab32527, Abcam), anti-CD32 (1:500, cat. no. ab23336, Abcam), anti-IL1B (1:100, cat. no. 12242, Cell Signaling Technology), anti-NOS2 (1:100, cat. no. ab15323, Abcam), anti-NF (1:1,000, cat. no. ab8135, Abcam), anti-IBA1 (1:500, cat. no. 019-19741, Fujifilm), anti-CD68 (1:100, cat. no. 14-0688, Invitrogen), anti-MBP (1:250, cat. no. MAB386, Millipore), anti-F4/80 (1:100, cat. no. MCA497G, Bio-Rad), and anti-ABCA1 (1:200, cat. no. NB400-105, Novus Biologicals). Appropriate secondary antibodies for flow cytometry and immunofluorescence were purchased from Invitrogen.
Anti il1b
Manufactured by Cell Signaling Technology
Anti-IL1B is a laboratory reagent that detects the cytokine Interleukin-1 beta (IL-1β). It is used as a tool in research applications to study the expression and function of IL-1β.
Automatically generated - may contain errors
Lab products found in correlation
Anti abca1, by Abcam (1 mentions)
Anti nos2, by Cell Signaling Technology (1 mentions)
Anti abca1, by Cell Signaling Technology (1 mentions)
Anti mbp, by Thermo Fisher Scientific (1 mentions)
Anti cd68, by Fujifilm (1 mentions)
Anti ccr7, by Abcam (1 mentions)
Anti actin, by Cell Signaling Technology (1 mentions)
Anti iba1, by Abcam (1 mentions)
Odyssey system, by LI COR (1 mentions)
Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions)
Anti tbk1, by Cell Signaling Technology (1 mentions)
Iblot 2 gel transfer device, by Thermo Fisher Scientific (1 mentions)
Mops buffer, by Thermo Fisher Scientific (1 mentions)
Anti irf3, by Abcam (1 mentions)
Supersignal west femto substrate, by Thermo Fisher Scientific (1 mentions)
Gapdh, by Abcam (1 mentions)
Anti β actin, by Abcam (1 mentions)
2 protocols using anti il1b
1
Antibody Characterization Across Techniques
2
Western Blot Analysis of Immune Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein samples were run on pre-cast NuPAGE™ Bis-Tris gels (Invitrogen) with 1 x MOPS buffer (Invitrogen) and transferred on nitrocellulose membranes, using iBlot®2 Gel Transfer Device (Invitrogen). Membranes were washed in Tris Buffered Saline with 0.1% Tween-X100 (TBS-T) and blocked with TBS-T containing 5% bovine serum albumin (BSA, Sigma-Aldrich). Membranes were incubated with primary antibodies at 4°C overnight. The following primary antibodies were used: GAPDH (1:5000; ab8245; Abcam), anti-β-Actin (1:5000; cat#6276; Abcam) anti-IRF3 (1:1000; D83B9; cat#4302S; Abcam), anti-TBK1 (1:1000; cat#3504; Cell Signaling Technology), anti- IL1B (1:1000; cat#12242; Cell Signaling Technology) and SQSTM1 (1:1000; cat#PM045; MBL). Membranes were washed in TBS-T and incubated with secondary antibodies (HRP-conjugated, DAKO) for 1 hour at room temperature in TBS-T containing 1% milk or BSA. The blots were developed with SuperSignal West Femto Substrate (Thermo Scientific) and captured with LI-COR Odyssey system (LI-COR Biosciences, Lincoln, NE, USA).
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