Serum glucose was measured using biochemical assay kits (Pars Azmoon, Iran). Insulin levels of serum and islet secretion samples were evaluated using ELISA assays kits (Monobind, USA) (The sensitivity of hormone detection per assay tube was 0.182 µIU/ml). HOMA-IR (homeostasis model assessment-estimated insulin resistance) was calculated according to the following formula: fasting insulin (µIU/dL) × fasting glucose (mg/dL)/405 [16 ]. Lipid profile and hepatic enzymes in serum samples were measured using biochemical assay kits (Pars Azmoon, Iran) and an Autoanalyzer device (BT3000, Italy).
Biochemical assay kits
Manufactured by Pars Azmoon
Biochemical assay kits are laboratory equipment designed to perform specific chemical analyses. These kits contain pre-measured reagents, buffers, and other necessary components to detect and quantify the presence or concentration of target analytes in samples. They are commonly used in various fields, including biochemistry, molecular biology, and clinical diagnostics, to study metabolic processes, enzyme activities, and other biomolecular interactions.
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Lab products found in correlation
2 protocols using biochemical assay kits
1
Serum Glucose, Insulin, and Lipid Evaluation
2
Hormonal and Antioxidant Assessment in Study
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The plasma levels of insulin, FSH, LH, and testosterone levels were measured by ELISA kits (DRG Instruments, Marburg, Germany). The hormone detection sensitivity was 1.27 mIU/mL for FSH, 0.856 mIU/mL for LH, and 0.287 nmol/L for testosterone. Glucose assessment was performed using biochemical assay kits (Pars Azmoon, Tehran, Iran), with a detection limit of 0.182 μIU/mL in each test tube. SOD (Randox Laboratories, Crumlin, UK) and MDA testing were performed using an ELISA assay kit (ZellBio, Lonsee, Germany). The assay detects the MDA level calorimetrically in a range of 0.78–50 µM with 0.1 µM sensitivity.
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