18 male 12-week-old Sprague-Dawley (SD) rats were purchased from SLAC Laboratories, SIBS, Shanghai, China. Animals were housed at an ambient temperature of 22 ± 2 °C, maintained under a normal 12-hour light/dark cycle, and allowed with access to food and water ad libitum. After 2 weeks of acclimatization, rats were randomly assigned to 3 groups (n = 6 per group), with one conventionally fed with normal chow diets (NCD; containing 10% fat) and the other two with high-fat diets (HFD; containing 40% fat). Four months later, one of the HFD groups were orally co-administrated with 150 mg/kg/day berberine chloride (BBR; Sigma-Aldrich, St. Louis, MO, USA) while the other group were continued with solely HFD for another 4 months. BBR was suspended in normal saline (NS) before use. The rats without drug interventions were treated with an equal volume of NS. Animal treatment lasted for 8 months; body weight and fasting blood glucose (FBG) were measured every 2 weeks at the overnight fasting condition. Blood glucose levels were measured by an electronic glucometer (Terumo, Tokyo, Japan). All animal procedures were performed in accordance with the ethical principles in animal research adopted by the Department of Laboratory Animal Science, JiaoTong University School of Medicine, Shanghai, China.
Berberine chloride bbr
Manufactured by Merck Group
Sourced in United States
Berberine chloride (BBR) is a naturally occurring compound found in various plants. It is a crystalline solid that is soluble in water and has a range of applications in research and laboratory settings. BBR serves as a reference standard and is commonly used in analytical testing, assay development, and scientific investigations.
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Lab products found in correlation
3 protocols using berberine chloride bbr
1
High-Fat Diet and Berberine Effects
2
Antibacterial Compound Preparation Protocol
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Berberine chloride (BBR) (chemical formula C20H18CINO4) purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA) was dissolved in DMSO (Biomus, Poland) and filtered through a 0.22-μm Millipore filter (Corning, USA). Vancomycin (VAN) was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). BHI medium was obtained from (BIOMAXIMA, Lublin, Poland).
3
Berberine Chloride Incorporation in Grass Carp Diet
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Berberine chloride (BBR, analytical reagent) was purchased from Sigma-Aldrich (United States). BBR was suspended in distilled water before being sprayed evenly onto grass carp feeds. The formulated grass carp diet included the following: fish meal, 5 g⋅kg-1; soybean meal, 215 g⋅kg-1; cottonseed meal, 80 g⋅kg-1; rapeseed meal, 200 g⋅kg-1; wheat flour, 180 g⋅kg-1; rice bran, 150 g⋅kg-1; lees powder, 50 g⋅kg-1; malt root, 50 g⋅kg-1; choline chloride, 20 g⋅kg-1; mineral mixture 20 g⋅kg-1; vitamin mixture, 30 g⋅Kg-1 (Yu et al., 2017 (link)).
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