Prominence i lc 2030c 3d system

Manufactured by Shimadzu

The Prominence-i LC-2030C 3D system is a high-performance liquid chromatography (HPLC) instrument manufactured by Shimadzu. It is designed for efficient and reliable separation, identification, and quantification of a wide range of chemical compounds. The system features a compact design, advanced software, and a range of modules to meet the needs of various analytical applications.

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Lab products found in correlation

Icr mice, by Charles River Laboratories (1 mentions) Av 400 mhz spectrometer, by Bruker (1 mentions)

2 protocols using prominence i lc 2030c 3d system

Pharmacokinetics of Compound 15 in Mice

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Animals: ICR mice (male, 4–6 weeks old) were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). Animals ate food and water freely and were given a daily schedule of dark–light cycle. Before tests, they were housed for at least 12 hours.
The pharmacokinetic analysis of 15 was conducted in male ICR mice which were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd. The animals were administered a single dose of 2 mg/kg 15 by i.v. after fasting overnight. Blood was collected and centrifuged immediately to isolate plasma. The plasma concentrations were determined using high performance liquid chromatography with HPLC analysis on a Shimadzu Prominence-i LC-2030C 3D system. Pharmacokinetic parameters were estimated by non-compartmental model using WinNonlin 8.2.

Xi J.J., Cao Y., He R.Y., Zhang J.K., Zhao Y.M., Tong Q., Bao J.F., Dong Y.C., Zhuang R.X., Huang J.S., Chen Y, & Liu S.R. (2023). Design, Synthesis and Biological Evaluation of Glycosylated Derivatives of Silibinin as Potential Anti-Tumor Agents. Drug Design, Development and Therapy, 17, 2063-2076.

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Synthesis and Characterization of Bioactive Derivatives

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The commercially obtained chemicals were used directly without further purification. Solvents were purified and distilled following the standard procedures. All the reactions were monitored by thin-layer chromatography (TLC). The NMR spectra were taken on a Bruker AV-400 MHz spectrometer (400 MHz for ¹H and 101 MHz for ¹³C) and chemical shifts were expressed in ppm downfield using tetramethylsilane as the internal standard. High-resolution mass spectra (HRMS) were performed on a VG ZAB-HS mass spectrometer under electron spray ionisation (ESI). All the derivatives for testing bioactivity were purified to >95% purity which was determined by HPLC analysis on a Shimadzu Prominence-i LC-2030C 3D system (column, InertSustain C18, 4.6 × 250 mm, 5 μM; mobile phase, gradient elution of methanol/H₂O (90:10); low rate, 1.0 ml/min; UV wavelength, 190 − 800 nm; temperature, 40 °C; injection volume, 10 μL). The detailed synthesis of compounds is presented in Supplementary Material.

Wang X., Deng K., Wang C., Li Y., Wang T., Huang Z., Ma Y., Sun P., Shi Y., Yang S., Fan Y, & Xiang R. (2020). Novel CDKs inhibitors for the treatment of solid tumour by simultaneously regulating the cell cycle and transcription control. Journal of Enzyme Inhibition and Medicinal Chemistry, 35(1), 414-423.

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