Isotonac 3

All chemicals used in the study were of analytical grade and were either procured from an Indian manufacturer (SRL India, HiMedia chemicals) or obtained from Sigma Aldrich (St Louis, MO), Thermo Fisher Scientific Inc (USA) etc. Mannan oligosaccharide (MOS), 1,3,5-trihydroxybenzene, phloroglucinol, D-xylose, ethylene diaminetetracetic acid (EDTA), BCA kit, hematoxylin, eosin, thiobarbituric acid, BSA, Mops, Sucrose, TBA, DTNB, NEM, neutral buffered formalin, decalcifying solution-Lite, were obtained from Sigma-Aldrich Chemical Co., St. Louis, MO, USA. Phosphate buffer saline (PBS), acetic acid, hydrochloric acid, and all other chemicals obtained were of analytical grade from SRL India. Reagents for hematology analyzer (Isotonac 3, Hemolynac 5, Hemolyzing Reagent, Hemolynac 3, Cleanac 3) were procured from Nihon Kohden, Japan.

EDTA blood from Mccp-infected animals was used to measure the white blood cell (WBC) and red blood cell (RBC) count using the Celltac α MEK-6450 (Nihon Kohden, Japan). For caprine blood samples the parameters in the settings menu were adjusted as follows: WBC sensitivity = 10, WBC threshold = 7, RBC sensitivity = 15, RBC threshold: 3, RBC AUTO = OFF, PLT threshold = 5. The blood samples were thoroughly mixed with an equal volume of buffer (ISOTONAC 3, Nihon Kohden, Japan) prior to measurement. Provided values for WBCs, RBCs, HGB (hemoglobin) and HCT (hematocrit) were doubled prior to analysis to account for the buffer dilution. The analysis was carried out using the QP-821V Data Management Software LITE (Nihon Kohden, Japan).