Thin layer chromatography (TLC) was performed with silica gel 60 F254 (1.05715.0001; Merck KGaA) to analyze crude extracts of MB. The extracts were developed with ethyl acetate (AcOEt):n-hexane at 1:3 for 9 cm. Then, the resulting chromatogram was visualized with a handheld UV (254/365 nm) lamp (UVGL-58; Analytik Jena AG) equipped with Chromato-Vue Cabinet (C-10; Analytik Jena AG) followed by treatment with 5% phosphomolybdic acid hydrate in ethanol solution and then heating to enhance visualization [phosphomolybdic acid staining at 200°C for enough time to distinguish coloration (~1 min)].
Uvgl 58
Manufactured by Analytik Jena
Sourced in United States, United Kingdom
The UVGL-58 is a compact, multi-purpose ultraviolet lamp designed for use in various laboratory applications. It features an adjustable long-wave (365 nm) and short-wave (254 nm) UV light source, allowing for versatile functionality. The UVGL-58 is a self-contained unit that can be easily operated.
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Lab products found in correlation
11 protocols using uvgl 58
1
TLC Analysis of Crude Extracts
2
UV-induced Radical Generation in AgNPs
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Fifty microliters (10 ng/µL) of the AgNPs was subjected to UV (365 nm) irradiation for 0.5, 1, 3, or 30 min. UV irradiation was performed using a Handheld UV Lamp (UVGL-58; Analytik Jena, CA, USA), which is generally used for the detection of DNA. After irradiation with UV, 10 µL of radical trapping reagent 5,5-dimethyl-1-pyrroline N-oxide (DMPO) (#LM-2110; Dojindo Laboratories, Kumamoto, Japan) was added to each sample followed by gentle mixing. Subsequently, the mixture was transferred to a 50 µL calibrated pipet (#2-000-050; Drummond Scientific Company, Pennsylvania, USA) and analyzed using a benchtop EPR (EMX-nano, Bruker Corporation, Massachusetts, USA) together with its proprietary software solution (Xenon). In this study, all experiments were performed in the same area where there was no external light.
3
Chromatographic Fractionation and Spectroscopic Analysis
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The MRC extract (2 g) was fractionated by open column chromatography (2.7 cm diameter × 44 cm high), packed with 20 g of RP-18 silica gel (Supelco, Germany), and eluted with a gradient system of H2O: CH3CN. Aliquots of 10 mL were collected with an initial system of 100% H2O (1–10) and polarity changes from CH3CN at 5% (11–15), CH3CN at 50% (16–17), and CH3OH at 100% (18). The fractions were analyzed by reverse-phase-thin layer chromatography with an elution system of 90:10 H2O:CH3CN and displayed in a UV lamp (UVP UVGL-58) at λ = 254 nm and λ = 365 nm. Aliquots with a similar chromatographic profile (13–15) were grouped and diluted in dimethyl sulfoxide to be analyzed by NMR of 1H and 13C spectra at 100 MHz, 2-dimensional (2-D) correlated spectroscopy (COSY), heteronuclear simple quantum coherence (HSQC), and heteronuclear multiple bond coherence (HMBC) at 400 MHz on Varian INOVA-400 equipment.
4
Tagging Pantoea sp. PPE7 with GFP
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The pGFP with the lacZ initiation codon from pUC19 was used for tagging the Pantoea sp. strain PPE7 with the gfp gene expressing the green fluorescent protein. Pantoea sp. strain was transfected by electroporation and the transfected cells were selected on LB agar containing ampicillin (50 μg ml−1). Green fluorescent colonies were visualized by using a portable UV lamp (UVGL-58; UVP, Upland, CA, USA). The strain expressing green fluorescent protein was named Pantoea sp. PPEG7.
5
SDS-PAGE with MALDI-TOF MS Imaging
6
UV-A Inactivation of FCV Lysate
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Aliquots (20 μL) of FCV-infected cell lysate were dropped onto a cover glass (Matsunami Glass Ind., Ltd.) and then subjected to UV-A irradiation as follows. The spots were irradiated with UV-A using a UV transilluminator UVGL-58 (UVP; Upland, CA, USA) for 0–30 min. The distance between the drops and UV transilluminator was maintained at 20 mm. The energy (mJ/cm2) of UV-A was estimated on the basis of color changes to UV indicators (UV label-S) (NiGK Corporation, Tokyo, Japan). The treated FCV-infected cell lysate samples were used for viral titration assays as described above.
7
Measuring Photosynthetic Light Spectrum
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The spectrum of reflected and scattered light was measured by an illuminance spectrophotometer (CL-500A; Konica Minolta, Tokyo, Japan) as the irradiance (W·m−2). The total spectrum from 360 to 780 nm was measured, and the PFD for each spectral condition was calculated using the following formula: PFD (μmol·m−2·s−1) = [irradiance (W·m−2) × spectrum (m) × 10−9]/[Planck’s constant (6.626 × 10−34; J·s) × speed of light (2.998 × 108; m·s−1) × Avogadro’s constant (6.022 × 1023; mol−1)] × 106. The PFD results were used to compare PDA plates with and without α-CD and activated carbon. The bottom of each culture plate was irradiated with UV light at 365 nm (UVGL-58, UVP, Upland, CA, USA) in a dark room. The plates for each sample condition were prepared at least in triplicate.
8
Fabrication of Magnetic Microparticle Arrays
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Once the elastomeric mold was manufactured, the wells of the PDMS were filled with a magnetic suspension. First, a small amount of photoinitiator 2-hydroxy-2-mehtyl-1-phenyl-propan-1one was mixed with ethoxylated trimethylolpropane triacrylate (ETPTA) (10 : 1 w/w ETPTA : photoinitiator, Sigma-Aldrich). The densities of the photoinitiator and ETPTA were 1.077 g mL À1 and 1.11 g mL À1 , respectively. Next, carbonyl iron (CI) particles (density 7.86 g mL À1 , grade EW BASF SE, Germany) at a volume fraction of 50 vol% were mechanically dispersed in the mixture using a centrifugal mixer. Then, the CI suspensions were spread over the PDMS mold with a spoon spatula. Most of the excess suspension was removed by scraping off the surface of the mold with a razor blade. Next, the mold was pressed gently onto a glass slide several times in order to completely clean the excess material. Afterwards, the filled PDMS wells were degassed for 5 minutes and reticulated for 4 hours under a UV-lamp (UVGL-58, UVP, UK) with wavelength of 254 nm and power of 6 Watt. Finally, the mold was rinsed with ethanol and then a stream of air was used to dry the surface.
9
Fingerprint Detection Using Synthetic Powders
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Fingerprints were placed on glass slides after the hands were in gloves for several minutes to make them sweaty. The prints were dried for 5-10 minutes before they were dusted with the synthesised fingerprint powders. Because only a small amount of powder was synthesised, all of the powder was poured over the fingerprint and by tapping against the glass slide the excess powder was removed. The dusted fingerprints were excited using a handheld commercial UV lamp (UVP, model UVGL-58) at 254 nm, due to availability. Since the complex shows absorption between 250-290 nm with a peak at 270 the use of 254 nm still allowed for excitation. The results were recorded in a dark room using a reflex camera (Nikon D3200 with a 105mm Sigma macro lens). Exposure times up to 60 seconds were used to pick up the luminescence of the fingerprint powder. It has to be mentioned that UV light of 254 nm is harmful and has to be handled with care [24] . In addition 254 nm radiation causes damage to any DNA that might be present in the fingerprint [25] and will therefore eliminate the possibility to examine the fingerprints for DNA of the donor.
10
Fluoride Detection Using Probe 4
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Filter paper (Whatman, 20 micron) was cut to 0.5×2 cm. Test strips were immersed into a dichloromethane solution containing 4 (2 mM) and dried in air. To detect fluoride in water, the pre-treated Filter paper was immersed into an unbuffered DI water solution (containing 2 mM CTAB) for 5 seconds and dried in air for 10 minutes.
The fluorescence on the Filter paper was observed and recorded under 365 nm using a hand held UV lamp (UVGL-58 from UVP).
For selectivity assays with probe 4 the synthetic aquifer sample was prepared with 2x of the final concentration of NaF in the assay. This sample was then mixed 10 mM acetate (pH = 4.8) containing 2 mM CTAB at a 50:50 (v/v) ratio. The resulting solution was measured directly with test-strips of probe 4 as described in the preceding paragraph.
The fluorescence on the Filter paper was observed and recorded under 365 nm using a hand held UV lamp (UVGL-58 from UVP).
For selectivity assays with probe 4 the synthetic aquifer sample was prepared with 2x of the final concentration of NaF in the assay. This sample was then mixed 10 mM acetate (pH = 4.8) containing 2 mM CTAB at a 50:50 (v/v) ratio. The resulting solution was measured directly with test-strips of probe 4 as described in the preceding paragraph.
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