Lc ms qqq 6410b

LC–MS equipment (LC–MS QqQ-6410B Agilent Technologies) comprised a chomatographic system (1260 Infinity Agilent Technologies) coupled with an Agilent Triple Quad mass spectrometer fitted with an ESI source. MS conditions were the following: MS range 100–1200 Da, MSn spectra were obtained using both positive and negative modes, nebulizer gas 45 Psi, gas temperature 325 °C, capillary voltage 4000 V.
HPLC analysis was carried out by an Agilent 1260 Infinity series. A Chomolith RP-18e column (4.6 mm ID, 50 mm length) (Merck) was used. Mobile phase consisted of (A) aqueous formic acid (0.1%) and (B) methanol. Gradient condition was; 0–8 min, linear gradient from 12 to 25% of B; 8–12 min, isocratic conditions at 25% of B; 12–16 min, linear gradient from 25 to 40% of B; 16–40 min, linear gradient from 40 to 50% of B, 40–50 min, linear gradient from 50 to 100% of B. Flow rate: 1 mL/min.

A Nexera HPLC system (Shimadzu, Japan), equipped with the double pump (LC-30AD), Auto sampler and column oven, was used to conduct the HPLC analysis (SIL-30AC). The column was a Chromolith RP-18 column (60 mm length, 5.6 mm ID) from Merck. Solvents A (0.3% formic acid aqueous form) and B (acetonitrile) were utilized to produce slope for the mobile phase, and the following conditions were utilized for gradient elution: 0–5 min, 5—20% of solution B; 5—10 min, 25% of solution B; 10—15 min, 25—35% of solution B; 15—20 min, 45—100% of solution B; 20—25 min, 100% of solution B. The volume of the injection was 5 L, while the rate of flow used for the separation was set to be 0.5 ml/min.. The Agilent Triple Quad mass spectrometer, which is a component of the LC–MS apparatus (LC–MS QqQ-6410B Agilent Technologies), was coupled to the chromatographic system (1260 Infinity Agilent Technologies). Following are the different parameters set for MS: MSn spectra: + ve and -ve modes, MS range: 100–1200 Da, gas temperature: 325 °C, nebulizer gas pressure: 45 psi and capillary voltage: 4000 V.