α kg colorimetric assay kit

The level of glutamine in the culture medium was measured with a Glutamine Assay Kit (Bioassay Systems, Hayward, CA, USA). Briefly, the cell medium was mixed with the enzyme after deproteinization with an ultracentrifugal filter. The absorbance at 565 nm was measured with a BioTek™ 800TS Absorbance Reader (BioTek) after incubation for 40 min in the dark. Glutamine consumption was calculated according to the following equation: Glutamine consumption = glutamine in fresh medium-glutamine in cell medium.
The levels of α-KG and ATP in cultured cells were assessed with α-KG colorimetric assay kit (BioVision, Milpitas, CA, USA) or CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, Madison, WI, USA) in accordance with manufacturer’s instructions.

The cultured GC cells were suspended in assay Buffer for 24 h, and then the glutamine, glutamate determination kit or α‐KG colorimetric assay kit (BioVision) were used to detect the concentrations of glutamine, glutamate or α‐KG base on the manufacturer's project.