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Pab7154

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PAB7154 is a lab equipment product. It is a tool designed for specific laboratory applications. No further details on its core function or intended use can be provided in a concise, unbiased, and factual manner.

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Lab products found in correlation

Ab6556, by Abcam (1 mentions) Alexa 555, by Thermo Fisher Scientific (1 mentions) Alexa 680, by Thermo Fisher Scientific (1 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) Anti prpf6, by Santa Cruz Biotechnology (1 mentions) Sc 48786, by Santa Cruz Biotechnology (1 mentions) Sc 53518, by Santa Cruz Biotechnology (1 mentions) Sc 514655, by Santa Cruz Biotechnology (1 mentions) A31570, by Thermo Fisher Scientific (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Anti ha, by Roche (1 mentions) F1804, by Merck Group (1 mentions) A21202, by Thermo Fisher Scientific (1 mentions) A21206, by Thermo Fisher Scientific (1 mentions) Irdye 800, by Rockland Immunochemicals (1 mentions) Anti flag, by Merck Group (1 mentions) Anti mouse igg fitc, by Jackson ImmunoResearch (1 mentions) Huc d, by Thermo Fisher Scientific (1 mentions) Arl13b, by Proteintech (1 mentions) Ab2182, by Abcam (1 mentions)

3 protocols using pab7154

1

Comprehensive Immunolabeling Protocol for RNA Splicing

Cited 1 time
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We used the following primary antibodies: rabbit polyclonal antibodies against SANS (RpAb) (29 (link),37 (link)), anti-SANS (mouse, monoclonal antibody; MmAb) (sc-514418, Santa Cruz), anti-FLAG (F1804, Sigma Aldrich), anti-PRPF6 (sc-48786, Santa Cruz), anti-HA (Roche), anti-GFP (ab6556, Abcam), anti-PRPF31 (PAB7154, Abnova), anti-hSNU114 (38 (link)), anti-Coilin (sc-55594, Santa Cruz), anti-SC35 (sc-53518, Santa Cruz), anti-SON (ATLAS, HPA023535), anti-SF3B1 (MmAb) (sc-514655, Santa Cruz), anti-SF3B1 (RmAb) (Will et al. 2001), anti-U5 52K (39 (link)), anti-SART1 (PA556663, Thermo Fisher) and anti-PRPF38 (40 (link)). Secondary antibodies were conjugated to the following fluorophores: Alexa488 (donkey-anti-rabbit, A21206, Molecular Probes,), CF 488 (donkey-anti-guinea pig, 20169-1, Biotrend,), Alexa488 (donkey-anti-mouse, A21202, Molecular Probes), Alexa 555 (donkey-anti-mouse, A31570, Molecular Probes), Alexa 568 (donkey-anti-rabbit, A10043, Invitrogen,) CF640 (donkey-anti-goat, 20179-1, Biotrend), Alexa 680 (donkey-anti-goat, A21084, Molecular Probes), Alexa 680 (goat-anti-rat, A21096, Molecular Probes) Alexa 680 (donkey-anti-rabbit, A10043), IRDye 800 (donkey-anti-mouse, 610–732-124, Rockland), Abberior STAR Orange (goat-anti-mouse, Abberior,), and Abberior STAR Red (goat-anti-Rabbit). Nuclear DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (1 mg/ml) (Sigma-Aldrich).
Yildirim A., Mozaffari-Jovin S., Wallisch A.K., Schäfer J., Ludwig S.E., Urlaub H., Lührmann R, & Wolfrum U. (2021). SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes. Nucleic Acids Research, 49(10), 5845-5866.
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2

Immunofluorescence analysis of iPSC-derived retinal organoids

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iPSC-derived retinal organoids were fixed in 4% paraformaldehyde at room temperature for 20 min. Post-fixation retinal organoids were incubated overnight with 30% sucrose in PBS, and then frozen and cryosectioned. The frozen sections were stained for a panel of retinal-specific antibodies. Antibodies against the following proteins were used at the indicated dilutions: RECOVERIN (Merck Millipore, AB5585, 1:800), NRL (Santa Cruz, sc-374277, 1:800), CRALBP (Abcam, ab15051, 1:500), HuC/D (ThermoFisher, A21271, 1:500), PKCα (BD Biosciences, 610107, 1:500), ARL13B (Proteintech, 17711-1-AP, 1:500), PRPF31 (Abnova, PAB7154, 1:500), SNRPB monoclonal antibody (Y12) (ThermoFisher, MA5-13449, 1:500). The following secondary antibodies were used: anti-mouse-IgG-FITC (Jackson Immuno Research, 715-095-151, 1:500), anti-mouse-IgG-Cy3 (Jackson Immuno Research/115-165-003, 1:500), anti-rabbit-IgG-Cy3 (Jackson Immuno Research/111-165-003, 1:500), anti-goat-IgG-FITC (Jackson Immuno Research/705-096-147, 1:500). Nuclei were labelled with blue-DAPI (ThermoFisher, 62248). All antibody details are shown in Supplementary Data 6.
Buskin A., Zhu L., Chichagova V., Basu B., Mozaffari-Jovin S., Dolan D., Droop A., Collin J., Bronstein R., Mehrotra S., Farkas M., Hilgen G., White K., Pan K.T., Treumann A., Hallam D., Bialas K., Chung G., Mellough C., Ding Y., Krasnogor N., Przyborski S., Zwolinski S., Al-Aama J., Alharthi S., Xu Y., Wheway G., Szymanska K., McKibbin M., Inglehearn C.F., Elliott D.J., Lindsay S., Ali R.R., Steel D.H., Armstrong L., Sernagor E., Urlaub H., Pierce E., Lührmann R., Grellscheid S.N., Johnson C.A, & Lako M. (2018). Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nature Communications, 9, 4234.
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3

Immunofluorescence Staining of Cellular Organelles

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Cells were fixed in 4% formaldehyde (Sigma, 47608) for 15 min at room temperature and permeabilised with 0.25% Triton X-100 (Sigma, T8787) for 15 min, followed by treatment with blocking solution (3% BSA in PBS, Sigma, A3311) for 30 min at room temperature. Cells were treated with primary antibodies anti-bestrophin (Abcam, ab2182, 1:300), anti-sodium potassium ATPase (Alexa Fluor® 488 conjugate) (Abcam, ab197713, 1:50), pericentrin (Abcam, ab28144, 1:500), MERTK (Bethyl, A300-222A, 1:200), ARL13B (Proteintech, 17711-1-AP, 1:500), collagen IV (Abcam, ab6586, 1:200), PRPF31 (Abnova, PAB7154, 1:500) and SNRPB monoclonal antibody (Y12) (ThermoFisher, MA5-13449, 1:500), overnight at 4 °C, and with secondary antibodies anti-rabbit FITC (Sigma, T9887, 1:500) or anti-mouse FITC (Jackson Immuno Research, 715-095-151, 1:500) and anti-mouse Cy3 (Jackson Immuno Research, 115-165-003, 1:500) or anti-rabbit Cy3 (Jackson Immuno Research, 111-165-003, 1:500) diluted in PBS for 1 h at room temperature. Washes with PBS were carried out between and after treatments. Finally, cells were treated with the nuclear stain-DAPI (Partec, 05-5005), and imaged using a Nikon A1R confocal microscope in combination with the associated NIS Elements software. All antibody details are shown in Supplementary Data 6.
Buskin A., Zhu L., Chichagova V., Basu B., Mozaffari-Jovin S., Dolan D., Droop A., Collin J., Bronstein R., Mehrotra S., Farkas M., Hilgen G., White K., Pan K.T., Treumann A., Hallam D., Bialas K., Chung G., Mellough C., Ding Y., Krasnogor N., Przyborski S., Zwolinski S., Al-Aama J., Alharthi S., Xu Y., Wheway G., Szymanska K., McKibbin M., Inglehearn C.F., Elliott D.J., Lindsay S., Ali R.R., Steel D.H., Armstrong L., Sernagor E., Urlaub H., Pierce E., Lührmann R., Grellscheid S.N., Johnson C.A, & Lako M. (2018). Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa. Nature Communications, 9, 4234.
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