Anti ly 6g pe cy7 clone 1a8

Manufactured by BioLegend

Sourced in United States

Anti-Ly-6G PE-Cy7 (clone 1A8) is a fluorescently conjugated monoclonal antibody that binds to the Ly-6G antigen. Ly-6G is a glycosylphosphatidylinositol-anchored protein expressed on the surface of mature neutrophils.

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Lab products found in correlation

Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Anti cd45 pe cy5 clone 30 f11, by BioLegend (2 mentions) Anti ly 6c apc cy7 clone al 21, by BioLegend (2 mentions) Cd11c fitc clone hl3, by BD (2 mentions) Anti cd3 pacific blue clone 17a2, by BioLegend (2 mentions) Anti granzyme b qa16a02, by BioLegend (1 mentions) Cd11b alexa fluor 700, by BioLegend (1 mentions) Anti foxp3 alexa fluor 488 clone mf23, by BD (1 mentions) Il 2 fitc clone jes6 5h4, by BD (1 mentions) Anti pd l1 pe clone 10f 9g2, by BioLegend (1 mentions) Anti pd 1 apc clone 29f 1a12, by BioLegend (1 mentions) Anti cd45 pe clone 30 f11, by BD (1 mentions) 7 aminoactinomycin d, by Thermo Fisher Scientific (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions)

Spelling variants of this product

Clone 1A8 (12 citations) Ly-6G-PE-Cy7 (1A8), (3 citations) PE-anti-Ly-6G (1A8, (3 citations)

3 protocols using anti ly 6g pe cy7 clone 1a8

Multiparametric Flow Cytometry Analysis

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The Abs used for cell-surface staining included: anti-CD3 Pacific Blue (clone 17A2), anti-CD8 PE Dazzle 594 (clone 53.6.7), anti-CD45 PE-Cy5 (clone 30-F11), anti-Ly-6G PE-Cy7 (clone 1A8), anti-Ly-6C APC-Cy7 (clone AL-21), anti-PD-L1 PE (clone 10F.9G2), anti-PD-1 APC (clone 29F-1A12), CD11b Alexa Fluor 700 (clone M1/70), anti-CD4 Brilliant Violet 570 (clone RM4-5), anti-CD44 PE-Cy7 (clone IM7), CD25 APC (clone 3C7) (Biolegend, San Diego, CA, USA), and CD11c FITC (clone HL3) (BD Biosciences, San José, CA, USA). The abs for intracellular staining were anti-FoxP3 Alexa Fluor 488 (clone MF23) (BD Biosciences) and anti-CTLA-4 PE (clone UC10-4F10-11) (Biolegend). A LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies, Thermo Scientific, Eugene, OR, USA) was used for dead cell exclusion. The abs used for intracellular cytokines evaluation were anti-IFNγ Alexa Fluor 700 (clone XMG1.2), TNFα PE-Cy7 (clone MP6-XT22), IL-2 FITC (clone JES6-5H4) (BD Biosciences), anti-perforin (clone S16009A), and anti-granzyme B (QA16A02) (Biolegend).

Lasso P., Rojas L., Arévalo C., Urueña C., Murillo N., Barreto A., Costa G.M, & Fiorentino S. (2022). Tillandsia usneoides Extract Decreases the Primary Tumor in a Murine Breast Cancer Model but Not in Melanoma. Cancers, 14(21), 5383.

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Multi-parameter Immune Cell Profiling

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The following Abs were used for cell surface staining: anti-CD3 Pacific Blue (clone 17A2), anti-CD8 PE Dazzle (clone 53.6.7), anti-CD45 PE-Cy5 (clone 30-F11), anti-Ly-6G PE-Cy7 (clone 1A8), anti-Ly-6C APC-Cy7 (clone AL-21), anti-PD-L1 PE (clone 10F.9G2), anti-PD-1 APC (clone 29F-1A12), CD11b Alexa Fluor 700 (clone M1/70), B220 PE (clone RA-3-6B2) (Biolegend, San Diego, CA, USA), anti-CD45 PE (clone 30-F11), anti-CD4 PerCP (clone RM4-5), Gr1 PE-Cy7 (clone RB6-8C5), CD11c FITC (clone HL3), and anti-CD44 APC (clone IM7) (BD Biosciences, San José, CA, USA). A LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies, Thermo Scientific, Eugene, OR, USA) was used for dead cell exclusion.

Lasso P., Gomez-Cadena A., Urueña C., Donda A., Martinez-Usatorre A., Romero P., Barreto A, & Fiorentino S. (2020). An Immunomodulatory Gallotanin-Rich Fraction From Caesalpinia spinosa Enhances the Therapeutic Effect of Anti-PD-L1 in Melanoma. Frontiers in Immunology, 11, 584959.

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Granulocyte Identification by Flow Cytometry

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Cell suspensions were generated with 2% FCS in PBS and then incubated with the appropriate antibody cocktail for 40 min in the dark and on ice with agitation. The myeloid antibody cocktail was made in Fc (fragment crystallizable antibody region) block and contained anti-F4/80-Alexa Fluor 647 (clone BM8; AbD Serotec), anti-CD115-PE (clone AFS98; BioLegend), anti-Ly6G PE/Cy7 (clone 1A8; BioLegend), anti-Ly6C APC/Fire 750 (clone HK1.4; BioLegend), and anti-CD11b BV510 (M1/70; BioLegend). Cells were washed and resuspended in 100 µl 2% FCS in PBS. Specificity of staining was determined by comparison to unstained cells and appropriate isotype control cocktail. Five minutes before analysis, 5 µg/ml 7-amino actinomycin D (Life Technologies) was added to each tube to allow for the exclusion of dead cells. Cells were examined via flow cytometry on a Cytoflex flow cytometer (Beckman Coulter), and data were analyzed using FlowJo version 10 (Tree Star Data Analysis Software). Analyses were performed on live (7-amino actinomycin D negative) cells after cell aggregate exclusion. Granulocytes were gated as CD11b⁺Ly6G⁺.

Starobova H., Monteleone M., Adolphe C., Batoon L., Sandrock C.J., Tay B., Deuis J.R., Smith A.V., Mueller A., Nadar E.I., Lawrence G.P., Mayor A., Tolson E., Levesque J.P., Pettit A.R., Wainwright B.J., Schroder K, & Vetter I. (2021). Vincristine-induced peripheral neuropathy is driven by canonical NLRP3 activation and IL-1β release. The Journal of Experimental Medicine, 218(5), e20201452.

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