Elecsys nt probnp 2 kits

Serum samples for routine laboratory measurements at baseline, and at 6 months and 2 years after implantation were collected and analyzed with the Cobas Integra 400 Plus^® (Mannheim, Germany) clinical chemistry system using absorbance photometric and turbidimetric techniques. The following Roche Diagnostics (Mannheim, Germany) reagents were used: creatine kinase (CK); creatine kinase MB isoenzyme (CKMB); creatinine; uric acid; BUN; CRP; glutamic oxaloacetic transaminase (GOT); glutamic pyruvate transaminase (GPT); gamma-glutamyl transferase (GGT); alkaline phosphatase (ALP); lactate dehydrogenase (LDH); total bilirubin; total protein; albumin; cholesterol; triglyceride; high-density lipoprotein (HDL); low-density lipoprotein (LDL); and glucose.
Aliquots for NT-proBNP were processed within 2 h of sampling and were stored frozen at −80 °C until the measurements. NT-proBNP levels were measured through electrochemiluminescence with a Cobas e 411^® analyzer (Mannheim, Germany) using Roche Elecsys NT-proBNP II kits (Cat. no.: 04842464190, Mannheim, Germany).

We collected serum and ethylenediaminetetraacetic acid plasma samples for biochemical measurements at baseline and 6 months after CRT implantation. The qualitative blood counts were measured on the day of blood sampling as the part of the routine clinical evaluation. Aliquots for the NT-proBNP were processed within 2 h of sampling and were stored frozen at −80°C until the later measurements. Blood cell counting was performed by using the Symex XS-1000i (Kobe, Japan) system with fluorescent flow cytometry technology. The absolute numbers of the WBC subgroups were automatically calculated by the analyser then displayed their relative percentages to the total WBC counts. The levels of the NT-proBNP were measured with electrochemiluminescence technology by Cobas e 411 analyser (Mannheim, Germany) using Roche Elecsys NT-proBNP II kits (Cat. No.: 04842464190, Mannheim, Germany).