Ch25h

NSG, WT, Ch25h^−/−, Rag1^−/−, Cd8 Cre and OT-I mice were purchased from Jackson Laboratory and used for study directly after importing into facility. The conditional Ch25h allele was created by flanking the single exon of the Ch25h gene with the loxP sites inserted into the non-conservative regions (Lu et al., 2021 (link)). OT-I WT and OT-I Ch25h^−/− mice were generated by crossing OT-I mice with WT or Ch25h^−/− mice. Atf3^f/f mice were previously described (Wolford et al., 2013 (link)) and kindly provided by Dr. Tsonwin Hai (Ohio State University). Ch25h^ΔCD8 or Atf3^ΔCD8 or Ch25h;Atf3^ΔCD8 mice were generated by intercrossing Cd8-Cre mice with conditional Ch25h^f/f or Atf3^f/f mice.
All animal experiments were performed using both male and female littermates (mated in-house) of 6–8 weeks of age. All mice except NSG were in the C57BL/6J background. Mice were housed in a specific pathogen free (SPF) condition (12 hr light/12 hr dark cycle, temperature 20–25°C) and had free access to water and chow (ANIMAL SPECIALTIES AND PROVISIONS, Lab diet 5010). Animal health status was routinely checked by qualified veterinarians.

C57BL/6J (CD45.2) (Stock No: 00064), Ly5.2 (CD45.1) congenic C57BL/6 (B6) (Stock No: 002014), Villin^cre(Stock No: 031792), Villin^cre/ERT2 (stock No: 020282), Ch25h^−/− (Stock No: 016263), Cyp27a1^tm1Elt (Stock No: 009106), Aicda^cre (Stock No: 007770), Rosa26^{flox-Stop-flox-tdTomato} (AI14)(Stock No: 007914), C57BL/6-Tg(UBC-GFP)30Scha/J (Stock No:004353), B6;129S-Nr1h1^tm1Djm/J(stock No:014635), Rosa26^{Stop-Cas9-EGFP}(Stock No:024858),Ch25h^flox/flox, Prdm1^YFP(Stock No: 008828), muMT(stock No: 002288), B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (Stock No 007914) were purchased from the Jackson Laboratory. Gpr183^flox/flox, C57BL/6 (B6) Ebi2^GFP/+ were previously described^{51 ,52} . Ch25h floxed mice were generated by inserting loxP sites 690bp 5’ and 20bp 3’ of the single coding exon. LoxP sites were inserted using CRISPR-EZ⁵³ using ultramer guides (Dharmacon). Homology arms flanking each loxP element were 500–600bp. All mice were bred and maintained under standard 12:12 hours light/dark conditions, at the temperature of 18–23°C with 40–60% of humidity and housed in specific pathogen-free (SPF) facility at the University of Massachusetts Chan Medical School. Unless noted, both female and male mice were analyzed at 7–12 weeks of age. All procedures were conformed to ethical principles and guidelines approved by the UMass Chan Institutional Animal Care and Use Committee.