Lipid peroxidation mda assay kit

For measurement of blood indexes, GTT and ITT were examined as described previously (after 6 h fasting), while other biomedical indexes were measured under fed conditions. Female WT and db/+ mice were sacrificed on GD 20. Samples of blood were centrifuged at 1000 g for 10 min at 4°C to collect serum samples, which were frozen at -80°C for subsequent analyses. Liver tissues were collected and frozen at -80°C for assessments of biochemical indexes. The TCh, LDL, high-density lipoprotein (HDL), MDA and TG levels were evaluated with Total Cholesterol Assay Kit (STA-384, Cell Biolabs), LDL/VLDL and HDL Cholesterol Assay Kit (STA-391, Cell Biolabs), Lipid Peroxidation MDA Assay Kit (S0131, Beyotime, Shanghai, China), and Serum Triglyceride Quantification Kit (STA-396, Cell Biolabs), respectively. Atherogenic index was determined as previously established (29 (link)). Preparation of liver lysates was conducted following previously described protocol (27 (link)). The MDA, GPx, SOD, catalase (CAT), and glutathione (GSH) levels in the liver were determined using Lipid Peroxidation MDA Assay Kit (S0131), Total Glutathione Peroxidase Assay Kit (S0058), Total Superoxide Dismutase Assay Kit with NBT (S0109), Catalase Assay Kit (S0051), and Glutathione Reductase Assay Kit (S0055), obtained from Beyotime Biotechnology (Shanghai, China), respectively.

Approximately 40 mg of mice lung tissue was weighed before homogenizing in RIPA Lysis Buffer (Beyotime, Haimen, Jiangsu, China). Protein concentrations were measured using a BCA assay kit (Beyotime). The levels of malondialdehyde (MDA) in lung tissue homogenates were measured by lipid peroxidation MDA assay kits (Beyotime) to calculate MDA content per unit of protein weight according to the manufacturer’s instructions. The reduced glutathione (GSH) and oxidized glutathione (GSSG) levels in lung tissues were examined by a GSH and GSSG assay kit (Beyotime) according to the manufacturer’s instructions.

MnSOD activity and MDA levels were detected according to the manufacture's instruction using Mn-SOD Assay Kits (S0103, Beyotime Biotechnology, Jiangsu, China) and lipid peroxidation MDA assay kits (S0131, Beyotime Biotechnology, Jiangsu, China).