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Pnl1.1 nluc neo

Manufactured by Promega
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PNL1.1-Nluc-Neo is a lab equipment product. It functions as a reporter construct that enables the expression and detection of nanoluciferase (Nluc) in cells. The product contains the PNL1.1 promoter, the Nluc gene, and a neomycin resistance gene for selection purposes.

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Lab products found in correlation

Nano glo, by Promega (2 mentions) Fugene hd, by Promega (2 mentions) Pgl4.10 luc2 vector, by Promega (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Pgl4.10 luc2, by Promega (1 mentions)

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PNL1.1 [Nluc] (4 citations)

2 protocols using pnl1.1 nluc neo

1

Quantifying CD271 Promoter Activity

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The CD271 promoter region was amplified from the genome of HPCM2 cells using polymerase chain reaction and cloned into the pNL1.1-Nluc-Neo vector (Promega). 293 T cells were transfected with the plasmids using FugeneHD (Promega) according to manufacturer instructions, and 2 days after transfection, cells were lysed with Nanoglo (Promega), and luminescence was measured using a Synergy H1 system (Agilent Technologies). The pGL4.10[luc2] vector (Promega) was also transfected along with pNL1.1-Nluc-Neo for normalization.
Nakazato A., Mochizuki M., Shibuya-Takahashi R., Fujimori H., Fujii K., Saijoh S., Morita S., Yamazaki T., Imai T., Sato I., Satoh K., Yamaguchi K., Sugamura K., Yasuda J., Matsuura K., Shojaku H., Asada Y, & Tamai K. (2022). RELA is required for CD271 expression and stem-like characteristics in hypopharyngeal cancer. Scientific Reports, 12, 17751.
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2

BEX2 Promoter Activity Assay

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The BEX2 promoter region was amplified from the genome of HuCCT1 cells using a PCR method (Table S1). 293 T cells were transfected with pNL1.1-Nluc-Neo that included the BEX2 promoter sequence using FugeneHD (Promega, Madison, WI). Two days after transfection, cells were lysed with Nanoglo (Promega) and luminescence was measured using Synergy H1 (BioTek). pGL4.10[luc2] (Promega) was also transfected with pNL1.1-Nluc-Neo to normalize these values.
Tamai K., Nakamura-Shima M., Shibuya-Takahashi R., Kanno S.I., Yasui A., Mochizuki M., Iwai W., Wakui Y., Abue M., Yamamoto K., Miura K., Mizuma M., Unno M., Kawamura S., Sato I., Yasuda J., Yamaguchi K., Sugamura K, & Satoh K. (2020). BEX2 suppresses mitochondrial activity and is required for dormant cancer stem cell maintenance in intrahepatic cholangiocarcinoma. Scientific Reports, 10, 21592.
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