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Mda activity assay kits

Manufactured by Nanjing Jiancheng
Sourced in China

The MDA activity assay kits are designed to measure the activity of malondialdehyde (MDA), a marker of oxidative stress. The kits provide a simple and reliable method for quantifying MDA levels in various biological samples.

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3 protocols using mda activity assay kits

1

Colonic Tissue Antioxidant and Cytokine Analysis

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Colonic tissue was homogenized in 50 mM ice-cold PBS solution. The MPO, T-SOD, and MDA activity assay kits were used to determine antioxidant indexes (Jiancheng Bioengineering Institute, Nanjing, China). IL-1β, IL-6, and tumor necrosis factor-α (TNF-α) cytokines were quantified using eBioscience ELISA kits following the manufacturer’s instructions (San Diego, CA, USA).
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2

MDA Quantification in HK-2 Cells

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MDA levels in HK-2 cell were detected using MDA activity assay kits, following the manufacturer’s protocols provided by Nanjing Jiancheng Bioengineering Institute, China (C013-2-1). MDA content was detected at a wavelength of 450 nm. Subsequently, the concentration of MDA was standardized by the total protein concentration, which was determined using the Bradford assay.
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3

Quantifying Oxidative Stress Biomarker MDA

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MDA activity assay kits were used to detect MDA levels in kidney tissues as per the manufacturer's protocols (Nanjing Jiancheng Bioengineering Institute, C013-2-1, Nanjing, China). MDA contents were detected at 450 nm. MDA concentration was normalized by total protein concentration, which was measured using the Bradford method.
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