The level of ROS in LF tissue was assessed by a Tissue Reactive Oxygen Species (ROS) Detection Kit (Bestbio China) according to the instructions. The level of ROS in LF cells was assessed by a C11-BODIPY probe assay kit (Invitrogen) according to the instructions. 1 × 104 LF cells were seeded in 96-well plates and cultured for 30 minutes with 2 μM C11-BODIPY probe, and the amount of ROS was measured using a flow cytometer.
C11 bodipy probe assay kit
Manufactured by Thermo Fisher Scientific
The C11-BODIPY probe assay kit is a fluorometric tool used to detect and quantify lipid peroxidation in biological samples. The kit contains a C11-BODIPY reagent, which functions as a fluorescent probe that selectively binds to lipid peroxides. This enables the measurement of lipid peroxidation levels in a sample, providing insights into oxidative stress conditions.
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Lab products found in correlation
3 protocols using c11 bodipy probe assay kit
1
Quantifying Reactive Oxygen Species in LF
2
Mitochondrial DNA Copy Number and Oxidative Stress
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LF samples were homogenised to isolate total DNA. real-time quantitative PCR was performed on the mitochondrial gene cytochrome c oxidase subunit 1 (COX1) and the nuclear DNA product (b-actin). The relative mtDNA copy number was calculated by subtracting the cycle of the nuclear gene from the cycle of the mitochondrial gene.
The level of ROS in LF cells was assessed by a C11-BODIPY probe assay kit (Invitrogen) according to the instructions. 1 × 104 LF cells were seeded in 96-well plates and cultured for 30 min with a 2 μM C11-BODIPY probe, and the number of reactive oxygen species (ROS) was measured using a flow cytometer. The malondialdehyde (MDA) and glutathione (GSH) content in tissue homogenates and cell lysis were analyzed by a lipid peroxidation kit (Sigma, MAK085) and Glutathione Assay Kit (Sigma, CS0260) by the standard protocol.
The level of ROS in LF cells was assessed by a C11-BODIPY probe assay kit (Invitrogen) according to the instructions. 1 × 104 LF cells were seeded in 96-well plates and cultured for 30 min with a 2 μM C11-BODIPY probe, and the number of reactive oxygen species (ROS) was measured using a flow cytometer. The malondialdehyde (MDA) and glutathione (GSH) content in tissue homogenates and cell lysis were analyzed by a lipid peroxidation kit (Sigma, MAK085) and Glutathione Assay Kit (Sigma, CS0260) by the standard protocol.
3
Lipid ROS Measurement in KGN Cells
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The level of lipid ROS in KGN cells was assessed by using C11-BODIPY probe assay kit (Invitrogen) according to the instructions. Briefly, 5 ×104 KGN cells were seeded in 96-well plates and cultured with 1 μM C11-BODIPY probe for 30 min, and then the level of lipid ROS was read by flow cytometer.
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