Log In Sign up
The largest database of trusted experimental protocols

T cell isolation kit

Manufactured by STEMCELL
Visit Supplier
Sourced in Canada, United States

The T cell isolation kit is a laboratory product designed to isolate and enrich T cells from a variety of sample types. The kit utilizes magnetic beads coated with antibodies specific to T cell surface markers, allowing for the efficient separation and purification of T cells from complex cell mixtures.

Automatically generated - may contain errors

Lab products found in correlation

Hgm csf, by Thermo Fisher Scientific (2 mentions) Hil 4, by Thermo Fisher Scientific (2 mentions) 48 well plate, by BD (2 mentions) Mabs cd3 ucht1, by BD (2 mentions) Soluble cd28 cd28.2, by BD (2 mentions) Intracellular ifn γ 4s b3, by BD (2 mentions) Leukocyte activation cocktail, by BD (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Cd3 cd28 tetrameric antibody complex, by STEMCELL (1 mentions) Anti cd28 ab, by BioLegend (1 mentions) Anti cd3, by BioLegend (1 mentions) Gapdh, by Absin (1 mentions) X vivo 15 serum free hematopoietic cell medium, by Lonza (1 mentions) Easysep buffer, by STEMCELL (1 mentions) Nucleocounter nc 200, by ChemoMetec (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Cyclosporin a, by Merck Group (1 mentions) Quantikines, by R&D Systems (1 mentions) Anti tubb3 antibody, by BioLegend (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

EasySep T cell or B cell Isolation Kits EasySep Human Naive CD4+ or CD8+ T cell isolation kits EasySep™ Mouse CD8+ or CD4+ T Cell Isolation Kits CD4+ T cells isolation kit Cell isolation kits Isolation kits

17 protocols using t cell isolation kit

1

Generating AFP-specific TCR-T Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Production of AFP-specific TCR-T cells is similar to that described previously [33 (link)]. In brief, peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by density gradient centrifugation with Ficoll-hypaque. T cells were purified with T cell isolation kit (STEMCELL Technologies, Cambridge, MA) and stimulated with CD3/CD28 tetrameric antibody complex (STEMCELL Technologies, Cambridge, MA) for 24 h. Activated T cells were transduced with lentiviral particles containing the TCR gene. After 48 h, the expression of TCR was measured by flow cytometry and CD3/CD28 antibody complex was rinsed away. For TCR-T cells culture, RPMI-1640 medium containing 10% FBS and 50 IU/mL of interleukin-2 (IL-2) (Quanqi, Shandong, China) was refreshed every other day.
Chen A., Yu Z., Ma N., Lu X., Zhang Y., Xu W., Wang Y., Xie J., Qin Y., Mo G., Wu S., Hou J, & Zhu W. (2024). Atovaquone enhances antitumor efficacy of TCR-T therapy by augmentation of ROS-induced ferroptosis in hepatocellular carcinoma. Cancer Immunology, Immunotherapy : CII, 73(3), 49.
+ Open protocol
+ Expand
2

Characterization of IL-39 Signaling in T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine CD3+T cells were sorted from splenocytes of C57BL/6 mice, and human CD3+T cells were sorted from peripheral blood mononuclear cells (PBMCs) of healthy individuals using a T Cell Isolation Kit, according to the manufacturer’s protocol (StemCell Technologies, Vancouver, Canada). For T cell activation, plates were coated with 2 mg/ml anti-CD3 and 0.4 mg/ml anti-CD28 Abs (BioLegend, San Diego, CA) overnight. Activated T cells were cultured with various concentrations of mouse or human rIL-39 protein for 72 h. Equal amounts of protein were subjected to 10% SDS-PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane. After blocking with 3% BSA, the membrane was incubated with primary antibodies, including STAT1 (D1K9Y), Phospho-STAT1 (58D6), STAT3 (D3Z2G), Phospho-STAT3 (D3A7), and GAPDH (D4C6R) (CST, USA) at 4 °C overnight, followed by incubation with secondary antibodies (Absin, China) at room temperature for 2 h. All results were normalized to GAPDH expression, which was used as the loading control.
Lv K., Hu B., Xu M., Wan L., Jin Z., Xu M., Du Y., Ma K., Lv Q., Xu Y., Lei L., Gong H., Liu H., Wu D, & Liu Y. (2022). IL-39 promotes chronic graft-versus-host disease by increasing T and B Cell pathogenicity. Experimental Hematology & Oncology, 11, 34.
+ Open protocol
+ Expand
3

Isolation and Generation of Human PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human PBMCs were isolated from healthy donors using a buffy coat (Gulf Coast Regional Blood Center, Houston, Texas, USA) by Ficoll gradient centrifugation. T-cells were isolated from PBMCs by negative selection using a T-cell isolation kit (#17951, Stemcell Technologies, California, USA). DCs were derived from PBMCs by culturing with 20 ng/mL hGM-CSF (#300-03, PeproTech, USA) and 20 ng/mL hIL-4 (#200-04, PeproTech) for 7 days.
Hong B., Sahu U., Mullarkey M.P., Hong E., Pei G., Yan Y., Otani Y., Banasavadi-Siddegowda Y., Fan H., Zhao Z., Yu J., Caligiuri M.A, & Kaur B. (2023). PKR induces TGF-β and limits oncolytic immune therapy. Journal for Immunotherapy of Cancer, 11(2), e006164.
+ Open protocol
+ Expand
4

Allogeneic T Cell Activation by Dendritic and Natural Killer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Syngeneic immature DC and NK cells or nicDC and nicNK cells (1×105 DC: 2×105 NK/well) exposed to Alum or TLR agonists for 8 hrs were subsequently co-cultured in 48-well plates (Falcon, Franklin Lakes, NJ) at a 1:10 ratio with CFSE labeled allogeneic naïve T cells isolated from PBMC using T cell isolation kit (StemCell Technologies, Vancouver, Canada). On day 5, the proliferating cells were transferred into new plates and rested in IL-2-containing medium (5 ng/ml) up to 10 days. The cells were subsequently collected and stained with CD4 (L200), CD8 (SK1), and CCR7 (3D12) (primary co-cultures). The remaining T cells were transferred to plates pre-coated with 10 μg/ml mAbs CD3 (UCHT1) and 2 μg/ml soluble CD28 (CD28.2) (BD Biosciences, San Diego, CA) for 72 hrs. The T cells were then stimulated for 4–6 hrs with leukocyte activation cocktail (BD Biosciences) containing Brefeldin A before staining with CD4 (L200), CD8 (SK1), CCR7 (3D12), and intracellular IFN-γ (4S.B3) (BD Biosciences, San Diego, CA) (secondary co-cultures). The frequency and amount of IFN-γ were further analyzed using Flow Cytometry and ELISA.
Nouri-Shirazi M., Tamjidi S., Nourishirazi E, & Guinet E. (2018). Combination of TLR8 and TLR4 agonists reduces the degrading effects of nicotine on DC-NK mediated effector T cell generation. International immunopharmacology, 61, 54-63.
+ Open protocol
+ Expand
5

Isolation and Characterization of Human PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human peripheral blood mononuclear cells (PBMCs) (Lonza Cat #4W-270C) were thawed rapidly in a 37°C water bath until a small bit of ice was left in the vial. Thawed cells were added dropwise to EasySepTM buffer (Stem Cell Technologies Cat #20144). The cells were centrifuged at 300 RCF for 5 min at room temperature (RT). The supernatant was discarded and the cells were reconstituted in 50 mL of EasySepTM buffer. Cell concentration and viability were evaluated using the NucleoCounter NC-200 (Chemometec, Denmark). The cells were centrifuged again at 300 RCF for 5 min at RT. The supernatant was discarded and the cells were reconstituted at 50 x 106 cells/mL in X-VIVOTM 15 Serum-free Hematopoietic Cell Medium (Lonza Cat #04-418Q). A sample was aliquoted for immune-phenotype staining. T cells were isolated using a T cell isolation kit (Stem Cell Technologies Cat #17951) by following the manufacturer's protocol. Post-T cell isolation, cell concentration, and viability were evaluated using the NucleoCounter NC-200 (Chemometec, Denmark), and a sample was aliquoted for immune-phenotype staining.
Gatla H., Uth N., Levinson Y., Navaei A., Sargent A., Ramaswamy S, & Friedrich Ben-Nun I. (2022). Enabling Allogeneic T Cell-Based Therapies: Scalable Stirred-Tank Bioreactor Mediated Manufacturing. Frontiers in Medical Technology, 4, 850565.
+ Open protocol
+ Expand
6

High-throughput Screening of Inhibitor Analogs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The compound library, InhiTinib, and its related analogs were provided by the IRIC HTS platform. Because the analog ligands are proprietary, their structures are not shown here. CD3-CD28 dynabeads, CellTraceTM, Hoechst 33342, nerve growth factor (NGF), and MitoSoxTM Red were purchased from Thermo Fisher. Human and murine interferon (IFN)-gamma Quantikines were purchased from R&D System. Annexin-V, propidium iodide (PI) and anti-TUBB3 antibody were purchased from Biolegend. Lipopolysaccharide, glial-derived neurotrophic factor (GDNF), cytosine arabinoside (AraC), and the immunosuppressive agent Cyclosporin A were purchased from Sigma. Collagenase A and dispase II were purchased from Roche Applied Sciences. T-cell isolation kit and lymphoprep® were purchased from StemCell Technologies. Z-VAD-FMK pan-caspase inhibitor was purchased from APExBIO. Western blot antibodies against human and murine poly(ADP-Ribose) polymerase-1 (PARP-1) (Cat. #sc-8007 and 46D11) were purchased from Santa Cruz and Cell Signaling, respectively; γH2AX (Cat. #05-636) from EMD Millipore; and H4 and tubulin (Cat. #ab6161) from Abcam SPHEROTM. AccuCount Particles were purchased from Spherotech, Inc.
El-Kadiry A.E., Abusarah J., Cui Y.E., El-Hachem N., Hammond-Martel I., Wurtele H., Thomas S., Ahmadi M., Balood M., Talbot S, & Rafei M. (2020). A Novel Sulfonyl-Based Small Molecule Exhibiting Anti-cancer Properties. Frontiers in Pharmacology, 11, 237.
+ Open protocol
+ Expand
7

T Cell and Myeloid Cell Transfer

Check if the same lab product or an alternative is used in the 5 most similar protocols
For T cell transfer, total T cells were isolated from lymph nodes and spleens using a T cell isolation kit (19851, STEMCELL Technologies). 2x106 T cells were adoptively transferred into each Rag1−/− mouse via retroorbital i.v. injection 2 days prior to start of the treatments. For myeloid cell transfer, bone marrow cells were harvested and washed twice with PBS. 2x106 cells were transferred via retroorbital i.v. injection 1 day prior to the start of the treatments.
Rao E., Hou Y., Huang X., Wang L., Wang J., Zheng W., Yang H., Yu X., Yang K., Bugno J., Ding X., Vokes E., Fu Y.X., Weichselbaum R.R, & Liang H.L. (2021). All-trans retinoic acid overcomes solid tumor radioresistance by inducing inflammatory macrophages. Science immunology, 6(60), eaba8426.
+ Open protocol
+ Expand
8

Adoptive T Cell Transfer for Immunization Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
For microarray analysis, one day prior to immunization with Ad5-OVA or Ad26-OVA, 5x103 CD4+ (OT-II) and 1x103 CD8+ (OT-I) T cells were purified “untouched” using the negative selection CD4 or CD8 T cell isolation kit (STEMCELL), following manufacturer’s instructions, and transferred into a CD45.1+ congenic mice (fig. S2A). For the in vivo suppression assay, 5x104 of IL-10+ tetramer+ or IL-10− tetramer+ CD4+ T cells were transferred one-day post immunization with 1010 vp of Ad5-Gag into CD45.1+ congenic mice (fig. 4D). For recombinant Listeria monocytogenes challenge study, 5x102 OT-I cells were isolated using T cell isolation kit (STEMCELL) and transferred to naïve mice. Ten days post immunization OT-I CD45.1 T cells were FACS sorted and 5x104 cell were transferred a day prior Listeria monocytogenes challenge.
Larocca R.A., Provine N.M., Aid M., Iampietro M.J., Borducchi E.N., Badamchi-Zadeh A., Abbink P., Ng’ang’a D., Bricault C.A., Blass E., Penaloza-MacMaster P., Stephenson K.E, & Barouch D.H. (2016). Adenovirus serotype 5 vaccine vectors trigger IL-27-dependent inhibitory CD4+ T cell responses that impair CD8+ T cell function. Science immunology, 1(5), eaaf7643.
+ Open protocol
+ Expand
9

Isolation and Sorting of Activated T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
T cells were isolated from single-cell suspensions of mediastinal lymph nodes and lungs using a T cell isolation kit as per the manufacturer’s instructions (Stem Cell). Cells were stained with surface antibodies and sorted on a FACS Aria IIU. CD4+ or CD8+ TCRβ+CD44hi cells that were EYFP+ or EYFP negative were sorted into Qiagen RLT buffer and stored at –80°C.
Finney G.E., Hargrave K.E., Pingen M., Purnell T., Todd D., MacDonald F., Worrell J.C, & MacLeod M.K. (2023). Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection. Discovery Immunology, 2(1), kyad014.
+ Open protocol
+ Expand
10

Isolation and Cultivation of Mouse T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse CD4+ or CD8+ T cells were isolated using EasySep Mouse CD4+ (catalog 19852) or CD8+ (catalog 19853) T Cell Isolation Kit (STEMCELL Technologies), per manufacturer’s instruction. Cells were cultured in RPMI 1640 medium, 10% heat-inactivated FBS, 2 mM glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2-mercaptoethanol (50 μM) at 37°C, 5% CO2.
Pan W., Sharabi A., Ferretti A., Zhang Y., Burbano C., Yoshida N., Tsokos M.G, & Tsokos G.C. (2020). PPP2R2D suppresses IL-2 production and Treg function. JCI Insight, 5(19), e138215.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!