Red blood cell lysis buffer

Manufactured by Thermo Fisher Scientific

Sourced in United States

Red blood cell lysis buffer is a solution used to selectively lyse or break down red blood cells while leaving other cell types intact. It is commonly used in sample preparation protocols to remove red blood cells and enrich for other cell populations, such as white blood cells, for further analysis or processing.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Lysis buffer (523 citations) Cell lysis buffer (171 citations) Red blood cells lysis buffer (6 citations) Red blood lysis buffer (2 citations) Red-cell lysis (2 citations)

259 protocols using red blood cell lysis buffer

Immunogenicity Evaluation of Vaccine

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood was collected at Study Day 21 by the retro-orbital route (prior to the boost immunization) under light sedation using isoflurane and at Study Day 42 via cardiac puncture after euthanasia. Blood was collected in Sarstedt Microvette capillary blood collection tubes and centrifuged to separate the serum. Serum was stored at −20 °C until analysis. On Study Day 42, mice were euthanized by controlled administration of carbon dioxide inhalation, followed by cervical dislocation, and spleens and bone marrow were removed aseptically. Lymphocytes were isolated from the spleen using manual disruption. Red blood cells contained in spleens were lysed using Red Blood Cell Lysis Buffer (eBioscience). Lymphocytes were used for cytokine secretion ELISA (interferon (IFN)-γ and IL-5) and cytokine ELISpot (IFN-γ and IL-5) assays as described below. Bone marrow was exposed by snipping ends of harvested femurs followed by rinse and centrifugation cycles in complete Roswell Park Memorial Institute (RPMI) medium (consisting of RPMI medium supplemented with 10% v/v fetal bovine serum (FBS) and penicillin-streptomycin). The red blood cells were removed with Red Blood Cell Lysis Buffer (eBioscience). Bone marrow-derived cells were used for B-cell ELISpot assay as described below.

Fisher K.J., Kinsey R., Mohamath R., Phan T., Liang H., Orr M.T., Lykins W.R., Guderian J.A., Bakken J., Argilla D., Ramer-Denisoff G., Larson E., Qi Y., Sivananthan S., Smolyar K., Carter D., Paddon C.J, & Fox C.B. (2023). Semi-synthetic terpenoids with differential adjuvant properties as sustainable replacements for shark squalene in vaccine emulsions. NPJ Vaccines, 8, 14.

+ Open protocol

+ Expand

Whole Blood Immune Modulation by BHB

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood samples were obtained from fasting healthy donors and incubated at 37°C. BHB cultivation was performed by adding 10 mM beta‐hydroxybutyrate (BHB; Sigma‐Aldrich, St. Louis, MO, USA) to the whole blood samples. Immune cells were stimulated using 100 ng/ml Lipopolysaccharide (LPS) for a duration of 3 h. Prior to subsequent analyses, red blood cell lysis was performed using Red Blood Cell Lysis Buffer (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.

Hirschberger S., Strauß G., Effinger D., Marstaller X., Ferstl A., Müller M.B., Wu T., Hübner M., Rahmel T., Mascolo H., Exner N., Heß J., Kreth F.W., Unger K, & Kreth S. (2021). Very‐low‐carbohydrate diet enhances human T‐cell immunity through immunometabolic reprogramming. EMBO Molecular Medicine, 13(8), e14323.

+ Open protocol

+ Expand

Satellite Cell Isolation and Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reagents purchased for isolation and culture of satellite cells included
Hanks' Balanced Salt Solution (HBSS, Welgene, Korea), Dulbecco’s
phosphate-buffered saline (DPBS; Thermo Fisher Scientific, Waltham, MA, USA),
glucose-free Dulbecco’s modified Eagle’s medium (DMEM; Thermo
Fisher Scientific), fetal bovine serum (FBS; Thermo Fisher Scientific),
Glutamax^TM supplement (Thermo Fisher Scientific), gelatin powder
(G9391; Sigma-Aldrich, St. Louis, MO, USA), 0.25%
trypsin-ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher Scientific), horse
serum (Thermo Fisher Scientific), antibiotic-antimycotic (×100) (Thermo
Fisher Scientific), human recombinant basic fibroblast growth factor (bFGF,
78003; Stemcell Technology, British Columbia, Canada), dimethyl sulfoxide (DMSO;
Sigma-Aldrich), and Red Blood Cell Lysis Buffer (Invitrogen, New Zealand). The
proliferation medium (PM) was a mixture of 30% FBS, 1% Glutamax,
and 5 ng/mL bFGF in glucose-free DMEM and used as a growth medium for the
satellite cells. Differentiation media (DM) was composed of 2% horse
serum in DMEM for inducing myotubes.

Joo S.T., Choi J.S., Hur S.J., Kim G.D., Kim C.J., Lee E.Y., Bakhsh A, & Hwang Y.H. (2022). A Comparative Study on the Taste Characteristics of Satellite Cell Cultured Meat Derived from Chicken and Cattle Muscles. Food Science of Animal Resources, 42(1), 175-185.

+ Open protocol

+ Expand

In Vivo Tumor Control Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

To examine in vivo local tumor control, the previously published animal setting was reused [16 (link)]. Briefly, 2 × 10⁶ A673 EwS cells (partly luciferase expressing, when indicated) were injected subcutaneously (s.c.) in the lower back of mice. Once the tumor was palpable, mice were randomized into indicated treatment groups. At day 3, mice were irradiated (3.5 Gy) and received (1) 1 × 10⁷ non-specific PBMC, (2) 5 × 10⁶ tgCD4⁺ T cells together with 5 × 10⁶ CD4-depleted non-specific PBMC, (3) 5 ×1 0⁶ tgCD8⁺ T cells together with CD8-depleted non-specific PBMC, or (4) 2.5 × 10⁶ tgCD4⁺, 2.5 × 10⁶ tgCD8⁺ T cells together with 5 × 10⁶ non-specific PBMC intraperitoneally (i.p.) on the next day. All mice received i.p. 1 × 10⁷ irradiated (80 Gy) IL-15-producing NSO cells biweekly until the end of experiment at day 17. Then, mice were sacrificed; s.c. tumors were explanted and weighed. Blood, spleen, and bone marrow were subjected to red-blood-cell lysis buffer (Invitrogen) according to manufacturer’s recommendations, stained with human anti-CD4/CD8, irrelevant/specific multimers, and negative controls, as mentioned above, before flow cytometric assessment.

Schober S.J., Thiede M., Gassmann H., Prexler C., Xue B., Schirmer D., Wohlleber D., Stein S., Grünewald T.G., Busch D.H., Richter G.H., Burdach S.E, & Thiel U. (2020). MHC Class I-Restricted TCR-Transgenic CD4+ T Cells Against STEAP1 Mediate Local Tumor Control of Ewing Sarcoma In Vivo. Cells, 9(7), 1581.

+ Open protocol

+ Expand

Single-Cell Transcriptome Profiling of Decidual and Placental Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Decidual and placental tissue were washed in phosphate-buffered saline with 100 IU/mL penicillin/streptomycin and sheared into tiny pieces. Decidual tissue were digested with collagenase type IV (0.5 mg/ml, Invitrogen) for 30 min while the resultant villous tissue was enzymatically digested with EDTA (Sigma) while stirring at 37°C for 9 min. The disaggregated cell suspension was passed through 70 and 40 µm mesh sieves (Biologix), centrifuged, and resuspended in 3 mL of red blood cell lysis buffer (Invitrogen) for 3 min to exclude any remaining red blood cells. The pelleted decidual cells and placental cells were resuspended in PBS and used for single‐cell 3′‐cDNA library preparation followed by the 10× Genomics Chromium Single‐Cell 3′ reagent kit using the manufacturer’s instructions.

Wei P., Dong M., Bi Y., Chen S., Huang W., Li T., Liu B., Fu X, & Yang Y. (2022). Identification and validation of a signature based on macrophage cell marker genes to predict recurrent miscarriage by integrated analysis of single-cell and bulk RNA-sequencing. Frontiers in Immunology, 13, 1053819.

+ Open protocol

+ Expand

Isolation of Normal Breast Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Normal breast epithelial cells (NBECs) were separated from tissue samples following surgical resection. Tissues were transported on ice in RPMI-1640 medium (Corning, Inc.) supplemented with 1% penicillin/streptomycin (Biological Industries), and used to isolate primary cells within 2 h. The tissues were washed three times with DPBS (Beijing Solarbio Science & Technology Co., Ltd.) and trimmed of excess fat, prior to cutting into 1-2 mm thick sections on ice. Type I collagenase (1.5 mg/ml, Sigma-Aldrich; Merck KGaA) was dissolved in DPBS containing 5% fetal bovine serum (FBS; Corning, Inc.) to digest tissues into cells. Tissues were dissociated by manual agitation for 20-40 min at 37˚C, and digestion was observed under a light microscope (magnification, x100). Cells were washed three times with DPBS containing 0.04% FBS to stop digestion and collected via centrifugation at 4˚C 3,000 x g for 5 min. Red Blood Cell lysis buffer (Invitrogen; Thermo Fisher Scientific, Inc.) was used to lyse erythrocytes on ice. Cells were re-washed three times with DPBS and cultured in RPMI-1640 medium supplemented with 10% FBS (Corning, Inc.), at 37˚C with 95% air and 5% CO₂.

Li H., Zhang H., Tan X., Liu D., Guo R., Wang M., Tang Y., Zheng K., Chen W., Li H., Tan M., Wang K., Liu R, & Tang S. (2021). Overexpression of ING3 is associated with attenuation of migration and invasion in breast cancer. Experimental and Therapeutic Medicine, 22(1), 699.

+ Open protocol

+ Expand

Isolation and Preparation of White Blood Cells and Oral Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For analysis of white blood cells (WBC), the buffy coat was collected and treated with red blood cell lysis buffer (Invitrogen). The resultant WBC suspension was washed with PBS, and fixed with 4% paraformaldehyde (PFA). A small aliquot of WBCs in a volume of 10 μl was applied to the surface of a silicon chip and the pipet tip was used to gently smear the cell suspension over the surface. Oral epithelial cells were obtained with a cytobrush, smeared on a silicon chip, and fixed with 4% PFA. All smeared cells were alcohol dehydrated and air-dried. The desired cell density was verified using differential interference contrast microscopy.

Steinhauser M.L., Guillermier C., Wang M, & Lechene C.P. (2014). Approaches to increasing analytical throughput of human samples with multi-isotope imaging mass spectrometry. Surface and interface analysis : SIA, 46(Suppl 1), 165-168.

+ Open protocol

+ Expand

Tumor and Spleen Pharmacokinetics Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

In the B16-F10 tumor PK/PD study, after a single intravenous bolus administration of THOR-707, the tumors and spleens were harvested at day 0 (2, 8, 12 h), 1, 2, 3, 5, 7 and 10, following CO₂ euthanasia. The tumor was separated into two halves, one half was weighed and frozen down in liquid nitrogen for tumor PK analysis.
Half of the tumors for flow cytometry analysis were processed right after collection. MACS mouse tumor dissociation kit (Miltenyi Biotec) was used to process tumor samples into single cells for flow cytometry analysis. Briefly, tumor samples were minced into small pieces, followed by mechanical and enzymatic digestion with Gentle MASC (Miltenyi Biotec).
Mouse splenocytes were dissociated by homogenizing spleens via straining using the plunger end of a syringe, then washed with 1× PBS, followed by red blood cell lysis with 1× red blood cell lysis buffer (Invitrogen, catalog #00-4333-57).

Ptacin J.L., Caffaro C.E., Ma L., San Jose Gall K.M., Aerni H.R., Acuff N.V., Herman R.W., Pavlova Y., Pena M.J., Chen D.B., Koriazova L.K., Shawver L.K., Joseph I.B, & Milla M.E. (2021). An engineered IL-2 reprogrammed for anti-tumor therapy using a semi-synthetic organism. Nature Communications, 12, 4785.

+ Open protocol

+ Expand

Apoptosis Evaluation of Suspended MSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

MSC were trypsinized from the culture flasks at 90% confluency or thawed after cryopreservation and re-suspended either in complete culture medium or perfusion fluid at a concentration of 500,000 MSC/ml. MSC were incubated in perfusion fluid in polypropylene tubes to avoid attachment of MSC to plastic. After 30 min or 2 h in suspension, MSC were submitted to a RBC lysis process to remove the large amount of RBC present in perfusion fluid. MSC incubated in suspension with culture medium were also subjected to RBC lysis to treat both groups in the same way. Briefly, 3 ml of red blood cell lysis buffer (Invitrogen, Carlsbad, CA, USA) was added to MSC and incubated for 20 min at room temperature (RT). MSC were then washed with PBS and stained with Annexin-V (PE) and ViaProbe (PercP) to assess the number of early and late apoptotic cells. Perfusion fluid was also added to attached MSC and incubated for the same time and then trypsinized and stained as mentioned. Cells were analyzed by flow cytometry (FACS Canto II, BD Biosciences, NJ, USA) and data were analyzed using Kaluza Analysis 1.5a (Beckman Coulter, Brea, CA, USA).

Sierra Parraga J.M., Rozenberg K., Eijken M., Leuvenink H.G., Hunter J., Merino A., Moers C., Møller B.K., Ploeg R.J., Baan C.C., Jespersen B, & Hoogduijn M.J. (2019). Effects of Normothermic Machine Perfusion Conditions on Mesenchymal Stromal Cells. Frontiers in Immunology, 10, 765.

+ Open protocol

+ Expand

Isolation of Intestinal Cells from Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

RPMI 1640 (Gibco, Cat. no. 11875–093, US) containing1 mM protease inhibitor (Solarbio, Cat. no. P6730, China), was used to transport ICC tissues. Tissues were digested with a dissociation enzyme cocktail prepared by dissolving 2 mg/mL Dispase II (Sigma-Aldrich, Cat.42613-33-2 US), 1 mg/mL Type VIII Collagenase (Sigma-Aldrich, Cat. no. C2139, US), and 1 unit/mL DNase I (NEB, Cat. no. M0303S, US) in PBS with 5% fetal bovine serum (FBS; Gibco, Cat. no. 16000–044, US) for 40 min at 37°C. The cells were dissociated and collected every 20 min, and then filtered using a 40 μm nylon cell strainer (Falcon, Cat. no. 352340, US). Red blood cell lysis buffer (Invitrogen, Cat. US) with 1 unit/mL DNase I was used to remove red blood cells. Finally, the cells were washed in PBS with 0.04% BSA (BSA; Sigma-Aldrich, Cat. no. B2064, US).

Shen Y., Xu S., Ye C., Li Q., Chen R., Wu W., Jiang Q., Jia Y., Zhang X., Fan L., Fu W., Jiang M., Chen J., Timko M.P., Zhao P, & Ruan J. (2023). Proteomic and single-cell landscape reveals novel pathogenic mechanisms of HBV-infected intrahepatic cholangiocarcinoma. iScience, 26(2), 106003.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!