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2 protocols using sodium nitrite

1

Synthesis and Characterization of Bioactive Compounds

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Anisaldehyde, 3,5-dichloroaniline, and monohydrated hydrazine were purchased from Merck (Darmstadt, Germany), phenol, ascorbic acid, urease (from jack bean), DPPH (2,2-diphenyl-1-picrylhydrazyl), acetylthiocholine iodide (ATCI), acetylcholinesterase, and 5,5-ditiobis(2-nitrobenzoic) acid (DTNB) were from Sigma-Aldrich (Steinheim, Germany). Sodium hypochlorite, and sodium salicylate were from Daejung (Siheung City, Korea). Lithium chloride, sodium nitrite and sodium nitroprusside were from Riedel de Haën (Seelze, Germany). Urea, ethylenediaminetetraacetic acid and thiourea were from BDH Labs (Cambridge, England).
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2

Analytical Method for Glyphosate Detection

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L-arginine, 3,3′-diamino-N-methyldipropylamine, [Ru(bpy)3] 2+ , sodium phosphate dibasic ≥99%, sodium phosphate monobasic monohydrate ≥99%, carbendazim, acetochlor, phosmet, glufosinate, N-(phosphonomethyl)iminodiacetic acid hydrate, (aminomethyl)phosphonic acid, D-(+)-glucose, calcium chloride, sodium nitrate and potassium sulfate were purchased from Merck (Darmstadt, Germany). 37% (w/w) hydrochloric acid was obtained from Scharlau (Barcelona, Spain). Sodium nitrite was purchased from Riedel-de-Haën (Seelze, Germany). Anti-glyphosate-IgG coupled magnetic particles (MP-Ab), glyphosate standards, diluent solution, washing solution, buffer assay and the derivatization reagent diluent, which is used to modify glyphosate in the standard solutions as well as in the spiked samples immediately before use, were purchased with the Glyphosate HS Kit from Eurofins Abraxis (Northampton (PA), USA). Purified water in a Millipore Milli-Q system was used in all experiments.
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