Research plus

Manufactured by Eppendorf

Sourced in Germany

The Eppendorf Research Plus is a manual single-channel pipette designed for precise and accurate liquid handling in laboratory settings. It features an ergonomic design and adjustable volume range to accommodate a variety of applications.

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Lab products found in correlation

Digiplus sonicator, by Bandelin (2 mentions) Cpa225d balance, by Sartorius (2 mentions) Mira3 feg sem, by TESCAN (2 mentions) Microscope slide, by Thermo Fisher Scientific (2 mentions) Methanol, by Merck Group (1 mentions) Sodium nitrate, by Sinopharm (1 mentions) Universal 320r, by Hettich (1 mentions) Glass screw cap headspace vials, by Restek (1 mentions) Ultrapure water, by Merck Group (1 mentions)

13 protocols using research plus

Quantifying Nanoparticle Release Kinetics

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Adhesive force between particles and substrates can be assessed qualitatively65 or quantitatively.66 ,67 (link) However, for release measurements several additional factors besides simple adhesion should be taken into account, such as the flow fluids across the surfaces. Therefore, a more praxis-related technique, as described below, was used in this work which simulated the in vivo conditions to qualitatively analyze the release properties.
The eADF4(κ16)-coated Se NPs immobilized on eADF4(κ16) films or eADF4(C16) films were fabricated into a 48-well plate, as mentioned above. For each type of films, 250 µL of MHB was added into each well of six sample wells and incubated at 37°C for 4 h. In three of these six sample wells, 150 µL of MHB was directly taken from each well and transferred to a 10 mL centrifuge tube. For the other three sample wells, 150 µL of MHB was taken after 5 times pipetting from the surface of films using a 1 mL pipette (Eppendorf^® Research^® Plus) and transferred to a 10 mL centrifuge tube. 350 µL HNO₃ was added into each of the centrifuge tubes and allowed to react overnight to dissolve the Se NPs. Then, the solution was diluted using water and analysed by inductively coupled plasma-optical emission spectrometry (ICP-OES, Varian 720-ES) to determine the Se ion concentrations.

Huang T., Kumari S., Herold H., Bargel H., Aigner T.B., Heath D.E., O’Brien-Simpson N.M., O’Connor A.J, & Scheibel T. (2020). Enhanced Antibacterial Activity of Se Nanoparticles Upon Coating with Recombinant Spider Silk Protein eADF4(κ16). International Journal of Nanomedicine, 15, 4275-4288.

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Microwave Sensor Position Sensitivity

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The sensitivity of different positions of the proposed microwave sensor was investigated. Equal amounts of deionized water (0.3 μL) were put in positions A, B, C, and D of the proposed sensor, using a precision pipette (Eppendorf, Research plus, 0.1−2.5 μL), as shown in Figure 7a. Figure 7b shows the measured transmission response of the test sample in different sensor positions. When the liquid sample was put in position C, the frequency deviation relative to the air was the most obvious, which is consistent with the presence of a strong electric field at position C, as shown in Figure 2b. The transmission characteristics of the microwave sensor with deionized water in different positions are given in Table 2. Thus, position C was chosen as the most sensitive position for the following experiments.

Zhang X., Ruan C., Haq T.U, & Chen K. (2019). High-Sensitivity Microwave Sensor for Liquid Characterization Using a Complementary Circular Spiral Resonator. Sensors (Basel, Switzerland), 19(4), 787.

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Oral Bacterial Inoculation Procedure

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For all bacterial strains used for oral inoculation, optical density at 600 nm and corresponding number of colony forming units were previously determined from overnight cultures. For oral inoculation, 10⁹ cells per strain per animal were resuspended in carboxymethyl cellulose and administered in an inoculum of about 100 µl to each animal of group B. To apply the inoculum into the oral cavity of each of the animals, we used a lab pipette with regular filter tips (Research plus, 1000 µl, Eppendorf, Hamburg, Germany). Oral inoculation was performed this way on 5 days per week for six consecutive weeks.

Blank E., Grischke J., Winkel A., Eberhard J., Kommerein N., Doll K., Yang I, & Stiesch M. (2021). Evaluation of biofilm colonization on multi-part dental implants in a rat model. BMC Oral Health, 21, 313.

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Electrode Characterization Using SEM

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Eppendorf pipettes (Research Plus) with OneTip pipette tips were used to prepare solutions. Solids were weighed using a Sartorius CPA225D balance. The surface morphology of the electrodes was analysed by scanning electron microscopy (SEM; Tescan MIRA3 FEG-SEM). Feature dimensions have been measured by built-in software. A Bandelin Sonorex Digiplus sonicator was used to disperse the ITO nanoparticles. A Carbolite furnace (ELF 11/14B/301) was used to anneal the electrodes.

Cobb S.J., Badiani V.M., Dharani A.M., Wagner A., Zacarias S., Olivera A.R., Pereira I.A, & Reisner E. (2022). Fast CO2 hydration kinetics impair heterogeneous but improve enzymatic CO2 reduction catalysis. Nature chemistry, 14(4), 417-424.

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Adsorption Kinetics of Zn2+ Ions

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The adsorption kinetics of Zn²⁺ were determined by adding 0.2 g of material into centrifuge tubes containing 100 mL Zn²⁺ solution (100 mg/L, pH 5); all centrifuge tubes were placed in a reciprocating shaker and shaken at a speed of 150 rpm for 24 h at 0.5, 1, 2, 3, 5, 10, 15, 30, 60, 90, 120, 180, and 240 min. Then 5 mL aliquots of samples were collected using a pipette (Eppendorf, Research Plus, 0.5–5 mL). The determination of Zn²⁺ was the same as in the adsorption isotherm experiment.

Xu L., Xing X, & Peng J. (2022). Removal of Zn2+ from Aqueous Solution Using Biomass Ash and Its Modified Product as Biosorbent. International Journal of Environmental Research and Public Health, 19(15), 9006.

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Electrode Characterization Using SEM

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Pharmaceutical Droplet Deposition and Evaporation

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Microscope slides (76 × 26 mm, pre-cleaned, cut edges; Thermo Scientific, Gerhard Menzel B.V. & Co. KG, Braunschweig, Germany) were degreased by washing them with a dishwasher liquid, then thoroughly rinsed with hot tap water, and placed in 4 consecutive purified water baths. Each slide was wiped dry with a laboratory wiper (KIMTECH science, Kimberly-Clark Professional, Roswell, Canada) just before droplet deposition. 3 μl droplets of the tested pharmaceutical preparation were deposited on the slides in two parallel rows, 7 droplets per row, by the use of a micro-pipette of 20 µl capacity (Eppendorf Research Plus, Eppendorf, Hamburg, Germany).
Evaporation took place in an incubator (KBF 720, cooled incubator with controlled humidity system, WTB Binder Labortechnik GmbH, Tuttingen, Germany) with an inner plexi-glass-chamber with a semi-permeable cover placed on a vibration absorbing basis. The Microscope slides with droplets were placed in the inner-chamber and left for evaporation in 26 °C and 44%rH for 1 hour. The slide distribution inside the chamber followed a quasi-randomization design in order to provide a uniform arrangement of the samples within the rows (Fig. 2).

Kokornaczyk M.O., Würtenberger S, & Baumgartner S. (2020). Impact of succussion on pharmaceutical preparations analyzed by means of patterns from evaporated droplets. Scientific Reports, 10, 570.

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Droplet Deposition and Evaporation Protocol

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Microscope slides (76 × 26 mm, pre-cleaned, cut edges; Thermo Scientific, Gerhard Menzel B.V. & Co. KG, Braunschweig, Germany) were de-greased by washing them with dishwasher liquid, then thoroughly rinsed with hot tap water, and placed in four consecutive purified water baths. Each slide was wiped dry with a laboratory wiper (KIMTECH science, Kimberly-Clark Professional, Roswell, Canada) just before droplet deposition. 2.6 μL droplets of the tested samples were deposited on the slides in two parallel rows, seven droplets per row, using a micropipette of 20 μL capacity (Eppendorf Research Plus, Eppendorf, Hamburg, Germany). Evaporation took place in an incubator (KBF 720, cooled incubator with controlled humidity system, WTB Binder Labortechnik GmbH, Tuttlingen, Germany) with two inner plexi-glass chambers, each covered with a semi-permeable foam and placed on a vibration-absorbing base. The Microscope slides with droplets were placed in the inner-chambers and left for evaporation in 26°C and 44% rH for 1 hour. The slide distribution inside the chambers followed a quasi-randomization design to provide a uniform arrangement of the samples within the rows.

Kokornaczyk M.O., Würtenberger S, & Baumgartner S. (2023). Self-assembled Patterns Formed in Evaporating Droplets to Analyze Bi-component Homeopathic Preparations in the Low Dilution Range. Homeopathy, 112(3), 152-159.

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Astaxanthin Extraction and Characterization

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Experimental materials: agar, sodium nitrate, sodium acetate, sodium chloride, sodium hydroxide and dichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd., the purity was analytically pure; methanol was a chromatographically pure reagent (≥99.9%, Sigma); Astaxanthin Standard (CAS: 472-61-7, Aladdin); Ultrapure water.

Experimental equipment: Renishaw in-Via-Reflex (London, England), RXZ intelligent artificial climate chamber (Ningbo Jiangnan Instrument Factory, China), Heal Force Neofuge 15R desktop high-speed refrigerated centrifuge (Likang Biomedical Technology Holdings Co., Ltd., China), DKZ series electric thermostatic oscillation sink (Shanghai Yiheng Technology Co., Ltd., China), SCILDGEX centrifuge, Eppendorf 5418 high speed desktop centrifuge (Eppendorf, Germany), high performance liquid chromatography (Shimadzu, Japan), LDZX-50KBS vertical pressure steam sterilizer (Shanghai Shenan Medical Instrument Factory, China), single-channel pipette Eppendorf Research plus (Eppendorf, Germany), blood cell counting board.

Shao Y., Gu W., Jiang L, & Zhu Y. (2019). Study on the Visualization of Pigment in Haematococcus pluvialis by Raman Spectroscopy Technique. Scientific Reports, 9, 12097.

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Point-of-Care Blood Plasma Analysis

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80 μl of capillary blood were collected from the participants’ earlobe and immediately centrifuged (Universal 320 R, Andreas Hettich GmbH, Tufflingen, Germany) for 10 min at 3000 rounds per minute. After centrifugation, 10 μl of blood plasma was collected with a reusable pipette (Eppendorf Research Plus, Eppendorf, Hamburg, Germany), pipetted on a dry slide reagent, and analyzed in a point-of-care-testing system (DRI-CHEM Analyzer FDC NX500, Fujifilm Europe, Duesseldorf, Germany). Post-exercise samples were manually diluted in the form of a dilution series (1:2; 1:4; 1:8; 1:16; 1:32; 1:64), using a concentrated 0.9% sodium chloride solution. The lowest dilution was placed on the dry slide reagent for analysis, and if the value was not detectable, the next highest dilution was applied. Finally, the undiluted value was calculated by multiplying the diluted value with the dilution factor.

Schwiete C., Roth C., Skutschik C., Möck S., Rettenmaier L., Happ K., Broich H, & Behringer M. (2023). Effects of muscle fatigue on exercise-induced hamstring muscle damage: a three-armed randomized controlled trial. European Journal of Applied Physiology, 123(11), 2545-2561.

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