Antibodies against PI3K-p85, AKT, p-AKT (Ser473), IRS2, phosphorylated glycogen synthase kinase-3β (p-GSK3β) (Ser9), phosphorylated forkhead box O1 (p-FOXO1) (Ser256), AMPKα, p-AMPKα (Thr172) and liver kinase B1 (LKB1) were all purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-SIRT1 and anti-EPOR antibodies were obtained from Santa Cruz Biotechnology (Dallas, Texas, USA). Antibodies against PPARγ, GSK3β, FOXO1 and β-actin were obtained from Bioworld Technology (St. Louis Park, MN, USA). The anti-p-IRS2 (Ser731) antibody was from AnaSpec (Fremont, CA, USA).
Gsk3β
Manufactured by Bioworld Technology
Sourced in United States
GSK3β is a protein kinase enzyme that plays a key role in regulating cellular processes such as metabolism, cell structure, and cell survival. It is an essential component of various signaling pathways and is involved in the phosphorylation of a wide range of substrates. GSK3β is commonly used in research to study its function and its implications in various biological and medical applications.
Automatically generated - may contain errors
Lab products found in correlation
P gsk 3β, by Bioworld Technology (2 mentions)
P gsk3β ser9, by Cell Signaling Technology (1 mentions)
P ampkα thr172, by Cell Signaling Technology (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
β actin, by Bioworld Technology (1 mentions)
N cadherin, by Bioworld Technology (1 mentions)
β catenin antibody, by BD (1 mentions)
E cadherin, by Bioworld Technology (1 mentions)
Erlotinib, by LC Laboratories (1 mentions)
Gapdh, by Bioworld Technology (1 mentions)
C fos, by Santa Cruz Biotechnology (1 mentions)
Human rankl, by Thermo Fisher Scientific (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Phospho erk, by Cell Signaling Technology (1 mentions)
Phospho p38, by Cell Signaling Technology (1 mentions)
Phospho iκb, by Cell Signaling Technology (1 mentions)
Nfatc1, by Santa Cruz Biotechnology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
M csf, by Thermo Fisher Scientific (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
6 protocols using gsk3β
1
Western Blot Analysis of Insulin Signaling
2
Erlotinib Signaling Pathway Analysis
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Erlotinib was purchased from LC Laboratories, dissolved in DMSO and stored at −20°C. Lipofectamine 2000 transfection reagent was purchased from Life Technologies Co., Invitrogen. Rabbit polyclonal antibodies against p-p70S6K (Thr389) (70 kDa), p-GSK3β (Ser9) (46 kDa), p70S6K (85 and 70 kDa), mTOR (289 kDa), and rabbit monoclonal antibodies against p-S6 (Ser235/236) and S6 (32 kDa) were purchased from Cell Signaling Technology, Inc. Mouse monoclonal antibodies against vimentin (57 kDa) and actin (43 kDa) were purchased from Santa Cruz Biotechnology Inc. E-cadherin (130 kDa), N-cadherin (140 kDa), GSK3β (47 kDa), and GAPDH (36 kDa) antibodies were purchased from Bioworld Technology Inc. β-catenin antibody (92 kDa) was purchased from BD Transduction Laboratories. Rabbit polyclonal raptor antibody (149 kDa) was purchased from Bethyl Laboratories.
3
Osteoclastogenesis Signaling Pathway
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Human RANKL and M-CSF were obtained from Peprotech EC Ltd. (London, UK). Akt, phospho-Akt, p38, phospho-p38, JNK, phospho-JNK, ERK, phospho-ERK, IκB, and phospho-IκB were purchased from Cell Signaling Technology Inc. (Beverly, MA, USA). GSK3β, phospho-GSK3β, and Runx2 antibodies were purchased from Bioworld Technology Inc. (St. Louis. Park, MN, USA). NFATc1, c-Fos, phospho-Smad, Smad, and β-actin antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
4
Western Blot Analysis of BDNF Signaling
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At the end of experiment, all rats were sacrificed with 10% chloral hydrate solution (3.5 ml/kg; i.p.). Hippocampus and prefrontal cortex tissues were collected and lysed with radioimmunoprecipitation assay buffer (Applygen Technologies, Inc., Beijing, China), schizolysised for 20 min on ice and centrifuged at 12,000 × g for 10 min at 4°C. Protein samples were heated at 95°C for 8 min, separated by 12% SDS-PAGE and transferred to nitrocellulose membranes. Membrane were blocked for 2 h in TBST (25 mM Tris, 140 mM NaCl, 27 mM KCl and 0.02% Tween 20) containing 5% bovine serum albumin and incubated with primary antibodies specific for BDNF (ab108319, 1:200)(Abcam, Cambridge, MA, USA), TrkB (BS1431, 1:500), ERK (AP0491, 1:500), pERK (BS4621, 1:500), Akt (BS1502, 1:500), PI3K (BS3678, 1:500), pGSK3β (BS4084, 1:500) and GSK3β (BS1402, 1:500; Bioworld Technology, Inc., St. Louis Park, MN, USA), pCREB (#9198, 1:1,000) and CREB (#9197, 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C over night. Following three washes with TBST, membranes were incubated for 1 h at room temperature with horseradish peroxidase-labeled secondary antibodies (BS13278, 1:5,000; Bioworld Technology, Inc.), washed with TBST three times. Blots were developed using a electrochemiluminescence system (UVP LLC, Upland, CA, USA).
5
Investigating ILK-Mediated Signaling Pathways
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EJ cells, HEK 293 cells, pcDNA3.1(−)-myc-RI, pGEX-4 T-RI and pEGFP-C1-RI plasmids were conserved and prepared by our laboratory. pGEX-4 T-1 was bought from GE Healthcare China. pEYFP-N1vector was from Clontech. pCMV-3xflag-CMVTM-10 was purchased from Sigma. BALB/C nude (nu/nu) mice were obtained from Beijing HFK Bioscience Company (Beijing, PR China). FBS was from TBD Science (Tianjin, PR, China). DMEM/High glucose medium, RPMI 1640 medium and G418 were purchased from Gibco-BRL (Carlsbad, CA, USA). Lipofectamine 2000 was bought from Invitrogen, Inc., (Carlsbad, California). Monoclonal mouse antibody of anti-human ILK was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-human β-actin, CD31 antibody, Monoclonal primary rabbit antibody of anti-human PI3K, p-PI3K, PTEN, p- PTEN, Akt, p-Akt, GSK3β, p-GSK3β, mTOR, p-mTOR and β-catenin were obtained from Bioworld Technology, Inc. (St. Louis, USA). The rest of the primary antibodies are from Proteintech Group, Inc (Chicago, IL, USA). Cell Counting Kit-8 was bought from Genview Scientific, Inc (Craigieburn, VIC, AUS).
6
Wnt Signaling Pathway Protein Analysis
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Aβ (polyclonal, 1:200; Santa Cruz Biotechnology, USA), T-tau (polyclonal, 1:1000; Santa Cruz Biotechnology, USA), PS205 (polyclonal, 1:1000; Bioworld Technology, China), PS396 (polyclonal, 1:1000; Santa Cruz Biotechnology, USA), and PS231 (polyclonal, 1:1000; Bioworld Technology, China) are specific to tau phosphorylated at the residues indicated. GSK-3β (polyclonal, 1:1000; Bioworld Technology, China), GSK-3β Ser9 (link) (polyclonal, 1:1000; Bioworld Technology, China), Dvl (polyclonal, 1:100; Santa Cruz Biotechnology, USA), β-catenin (polyclonal, 1:1000; Boster Technology, USA), β-catenin (S33/37, polyclonal, 1:1000; Boster Technology, USA), Dkk-1 (polyclonal, 1:100; Bioworld Technology), and p53 (polyclonal, 1:100; Santa Cruz Biotechnology, USA) antibodies were used to detect endogenous levels of Wnt. Anti-GAPDH (1:10,000; Bioworld Technology) was used as the internal reference standard. Rabbit anti-p-tau (T231) (polyclonal, 1:25; Bioworld Technology) was used to label p-tau in the immunofluorescence assays.
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