Np40 cell lysis buffer 10

Tissue culture media, antibiotics, fetal calf serum (FCS) and NP40 cell lysis buffer (10×) were purchased from Life Technologies (San Giuliano Milanese, Italy); lipopolysaccaride (LPS) (Ultra-Pure LPS-EB from E. coli 0111:B4 strain), zymosan, Pam3CSK4 (VacciGrade™) and polyinosinic-polycytidylic acid (poly(I:C)) (high molecular weight) were from InvivoGen (Cayla-Invivogen Europe, Toulouse, France); BD CytoFix/CytoPerm and CytoFix were from BD Biosciences (SACCO srl, Cadorago (CO), Italy); Ro-106-9920 (Tocris-Cookson, Space Import–export srl, Milan, Italy); poly-l-lysine hydrobromide (mol wt 70,000–150,000), papain, DNase I (bovine pancreas), trypsin inhibitor, l-leucyl-l-leucine methyl ester (L-LME), SB202190, protease inhibitor cocktail, Pefabloc® SC (100 mM), LPS from E. coli 026:B6 (<5 % protein impurities), polymyxin B and all other biochemicals were purchased from Sigma-Aldrich (Milan, Italy) unless noted otherwise; Falcon tissue culture plasticware was purchased from BD Biosciences. Sterilin petri plastic dishes (10 cm Ø) were obtained from Sarstedt (Verona, Italy).

Tissue culture media, antibiotics, fetal calf serum (FCS), and NP40 cell lysis buffer (10×) were purchased from Life Technologies (San Giuliano Milanese, Italy); lipopolysaccaride (LPS) (Ultra-Pure LPS-EB from E. coli 0111:B4 strain; only activates TLR4), Pam₃CSK₄, and ethyl-(6R)-6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-ene-1-carboxylate (CLI-095 or TAK 242) were from InvivoGen (Cayla-Invivogen Europe, Toulouse, France); A740003 from Tocris Bioscience, Pittsburgh, PA, USA); poly-L-lysine hydrobromide (mol wt 70,000–150,000), papain, DNase I (bovine pancreas), trypsin inhibitor, L-leucyl-L-leucine methyl ester (L-LME), protease inhibitor cocktail, Pefabloc® SC (100 mM), CU-CPT22, and all other biochemicals were purchased from Sigma-Aldrich (Milan, Italy) unless noted otherwise; recombinant human Apo-SAA (consensus SAA molecule corresponding to human Apo-SAA1α, except for the presence of an N-terminal methionine, the substitution of asparagine for aspartic acid at position 60, and arginine for histidine at position 71) from Peprotech (endotoxin level < 0.1 ng/μg protein; London, UK); QIAzol from Qiagen (Milan, Italy. Falcon tissue culture plastic-ware were purchased from BD Biosciences. Sterilin Petri plastic dishes (10 cm Ø) were obtained from Sarstedt (Verona, Italy).

Tissue culture media, N2 supplement, antibiotics and fetal calf serum (FCS) and NP40 cell lysis buffer (10×) were obtained from Life Technologies (San Giuliano Milanese, Italy); poly-D-lysine hydrobromide (mol wt 70,000–150,000), poly-L-lysine hydrobromide (mol wt 70,000–150,000), cytosine β-D-arabinoside, 3,3′,5-triiodo-L-thyronine, L-thyroxine, rapamycin, luteolin, papain, DNase I (bovine pancreas), trypsin inhibitor, protease inhibitor cocktail, Pefabloc® SC (100 mM), Pluronic F68 and all other biochemicals were purchased from Sigma-Aldrich (Milan, Italy) unless noted otherwise; Falcon tissue culture plasticware was purchased from BD Biosciences (SACCO srl, Cadorago (CO), Italy). Sterilin petri plastic dishes (10 cm Ø) were from Sarstedt (Verona, Italy). Co-ultramicronized PEA/luteolin (10:1 mass ratio) and ultramicronized PEA were kindly provided by Epitech S.p.A., Saccolongo (PD), Italy.