The cell viability of transfected MKN-45 cells was assessed using the MTT Cell Proliferation Assay Kit (Beyotime, Shanghai, China) in line with the manufacturer’s guidance. Transfected MKN-45 cells (5 × 104 per well) were incubated with 10 μL of MTT solution (5 mg/mL prepared with PBS [pH 7.4]) at 37°C for 4 h. Then, stop solution was added to the cells and incubated at 37°C overnight. The optical density value was determined at 570 nm absorbance with the Thermo Scientific Microplate Reader Multiskan MK3 (Thermo Fisher Scientific).
Thermo scientific microplate reader multiskan mk3
Manufactured by Thermo Fisher Scientific
The Thermo Scientific Microplate Reader Multiskan MK3 is a versatile instrument designed for various absorbance-based assays. It is capable of reading standard 96-well microplates and can be used for a range of applications, including colorimetric, luminescent, and fluorescent measurements.
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2 protocols using thermo scientific microplate reader multiskan mk3
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Cell Viability Assessment in MKN-45 Cells
2
Cell Viability Assay of ASNR Manipulation
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The cell viability of MKN45 cells transfected with si-ASNR and MKN28 cells transfected with pcDNA3.1-ASNR was evaluated via MTT Cell Proliferation and Cytotoxicity Assay Kit (Solarbio, Beijing, China) based on the manufacturer’s guidance. Transfected MKN-45 cells and MKN28 cells (5 × 104/well) were incubated with MTT (20 μl/well) at 37°C for 4 h. Next, DMSO was added and mixed for 10 min to ensure the crystals were dissolved. The optical density value was measured at the absorbance 570 nm under Thermo Scientific Microplate Reader Multiskan MK3 (Thermo Fisher Scientific).
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