Gill s hematoxylin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Gill's hematoxylin is a staining solution used in histology and cytology laboratories. It is a regressive hematoxylin stain that is commonly used for the nuclear staining of cells and tissues. Gill's hematoxylin provides a blue-purple coloration to the nuclei, allowing for the visualization and identification of cellular structures.

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Lab products found in correlation

Avidin biotin peroxidase, by Vector Laboratories (3 mentions) Thimerosal, by Merck Group (3 mentions) Novared, by Vector Laboratories (3 mentions) Hrp polymer kit, by Lab Vision (3 mentions) Biotinylated secondary antibody, by Vector Laboratories (2 mentions) Abc reagent, by Vector Laboratories (2 mentions) Diaminobenzidine dab, by Merck Group (2 mentions) Vectastain elite abc kit, by Vector Laboratories (2 mentions) Casein, by Merck Group (1 mentions) Biotinylated goat anti rabbit igg antibody, by Vector Laboratories (1 mentions) Rnascope 2.5 hd reagent kit brown, by Advanced Cell Diagnostics (1 mentions) Cytoseal xyl, by Thermo Fisher Scientific (1 mentions) Bx51 light microscope, by Olympus (1 mentions) Diaminobenzidine dab, by Agilent Technologies (1 mentions) Bx43 microscope, by Olympus (1 mentions) Protein block serum free, by Agilent Technologies (1 mentions) Antibody diluent, by Agilent Technologies (1 mentions) Dp80 camera, by Olympus (1 mentions) Liquid dab substrate chromagen system, by Agilent Technologies (1 mentions) Hm 325 microtome, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Hematoxylin (269 citations) Gill’s Hematoxylin I (4 citations) Gill’s hematoxylin and eosin Y (2 citations) Gill’s II hematoxylin (2 citations)

23 protocols using gill s hematoxylin

Immunohistochemical Analysis of Lung Tissue

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Human LGs from three donors were obtained from Advanced Tissue Services (Phoenix, AZ, USA). The LG were removed ≤24 hours after death. Tissues were preserved immediately in RNAlater and shipped at 4°C overnight. All donors were females, and their ages at the time of death were 62, 84, and 90 years. The LGs were embedded in paraffin, and 5-μm sections were prepared. Endogenous peroxidase activity on rehydrated sections was blocked by treating slides with 3% hydrogen peroxide in absolute methanol for 30 minutes. Antigen retrieval was performed for 40 minutes using 0.01 M citrate (pH 6.39) in a humidified heated chamber. Sections were blocked with 5 g/L casein (Sigma Aldrich) in PBS containing 0.5 g/L thimerosal (Sigma-Aldrich; cat# T5125-25G) for 30 minutes, incubated with primary antibodies, and diluted in casein buffer 1:50 overnight at 4°C. Biotinylated goat anti-rabbit IgG antibodies (Vector Labs, Burlingame, CA, USA) were used at a 1:300 dilution. Visualization was achieved using biotin/avidin-peroxidase (Vector Labs) and Nova Red (Vector Labs). Counterstaining was made with Gill's hematoxylin (Fisher Scientific, San Diego, CA, USA; CS400).

Basova L.V., Tang X., Umazume T., Gromova A., Zyrianova T., Shmushkovich T., Wolfson A., Hawley D., Zoukhri D., Shestopalov V.I, & Makarenkova H.P. (2017). Manipulation of Panx1 Activity Increases the Engraftment of Transplanted Lacrimal Gland Epithelial Progenitor Cells. Investigative Ophthalmology & Visual Science, 58(13), 5654-5665.

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Immunohistochemical Staining of DsRed

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Tissues were fixed immediately and embedded in paraffin in Scripps histology core facility, and 5 μm sections were prepared. Endogenous peroxidase activity on rehydrated sections was blocked by treating slides with 3% hydrogen peroxide in absolute methanol for 30 min. Antigen retrieval was performed for 40 min using 0.01 M citrate (pH 6.0) in a humidified heated chamber. Sections were blocked with 5 g/L casein in PBS containing 0.5 g/L thimerosal (Sigma-Aldrich; cat# T5125-25G) for 30 min, and incubated with anti-DsRed primary antibody, and diluted in casein buffer overnight at 4°Cand anti-dsRed primary antibodies were used for immunostaining. Biotinylated secondary antibodies (Vector Labs, Burlingame, CA) were used at a 1:300 dilution. Visualization was achieved using biotin/avidin-peroxidase (Vector Labs) and Nova Red (Vector Labs). Tissue was counterstained with Gill’s hematoxylin (Fisher Scientific, San Diego, CA; CS400).

Mauduit O., Aure M.H., Delcroix V., Basova L., Srivastava A., Umazume T., Mays J.W., Bellusci S., Tucker A.S., Hajihosseini M.K., Hoffman M.P, & Makarenkova H.P. (2022). A mesenchymal to epithelial switch in Fgf10 expression specifies an evolutionary-conserved population of ionocytes in salivary glands. Cell reports, 39(2), 110663.

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Single-molecule RNA in situ hybridization of adrenal tissue

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After fixation, adrenals were embedded in paraffin blocks and sectioned at 5 µm thickness. Single-molecule RNA in situ hybridization was performed using a RNAscope 2.5 HD Brown Reagent Kit (Advanced Cell Diagnostics, 322300), following manufacturer’s protocol. Target retrieval was performed for 7 min^{27 (link)}. Slides were counter-stained with 30% Gill’s Hematoxylin (Fisher Scientific, 23-245654) and mounted with Cytoseal XYL (Fisher Scientific, 22-050-262). The following probes were used: Fgfr2 (ACD, 454951), Shroom3 (ACD, 472221). Positive control Ppib (ACD, 313911) and negative control dapB (ACD, 310043) probes were used in every experiment to ensure sample quality.

Leng S., Pignatti E., Khetani R.S., Shah M.S., Xu S., Miao J., Taketo M.M., Beuschlein F., Barrett P.Q., Carlone D.L, & Breault D.T. (2020). β-Catenin and FGFR2 regulate postnatal rosette-based adrenocortical morphogenesis. Nature Communications, 11, 1680.

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Murine Muscle Sirius Red Staining

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Sirius Red staining was performed on murine muscle sections. Sections were hydrated in a series of 100%, 95%, and 80% ethanol gradient incubations of 3 minutes each. The sections were then washed in water and stained with Gills Hematoxylin (S5400‐1D, Fisher Scientific) for 10 minutes. After washing, the sections were incubated in Scott solution for 3 minutes, washed once again, and incubated in 0.1% Sirius Red/Picric Acid (SO‐674, Rowley Biochemical) for 30 minutes. The sections were then washed twice for 5 minutes in acidified water and dehydrated in a series of 80%, 95%, and 100% ethanol gradient incubations for 10 minutes each. The dehydration was followed by a 5‐minute incubation with Xylene and mounting with dibutylphthalate polystyrene xylene DEPEX medium.

Bugiardini E., Nunes A.M., Oliveira‐Santos A., Dagda M., Fontelonga T.M., Barraza‐Flores P., Pittman A.M., Morrow J.M., Parton M., Houlden H., Elliott P.M., Syrris P., Maas R.P., Akhtar M.M., Küsters B., Raaphorst J., Schouten M., Kamsteeg E., van Engelen B., Hanna M.G., Phadke R., Lopes L.R., Matthews E, & Burkin D.J. (2022). Integrin α7 Mutations Are Associated With Adult‐Onset Cardiac Dysfunction in Humans and Mice. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(23), e026494.

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Immunohistochemical Analysis of BCO1 and BCO2 in Rat Tissues

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Immunohistochemical staining was performed to determine the localization of BCO1 and BCO2 proteins in rat liver and intestinal cells. Formalin-fixed, paraffin-embedded rat liver and intestinal sections of 3–5 µm were prepared by Veterinary Histology Laboratory Services, The Ohio State University (Columbus, OH, USA). Before proceeding with staining, paraffin sections were first deparaffinized in xylene and rehydrated in graded ethanol. Sections were treated with 3% hydrogen peroxide to block endogenous peroxidase activity and blocked with 3% BSA and 1.28% NDS in PBS (pH 7.4). After blocking, sections were incubated with primary antibodies: rabbit anti-mouse BCO1, chicken anti-BCO2, goat anti-human desmin, and rabbit anti-villin. Tissues were then incubated with biotin-SP conjugated secondary antibodies: donkey anti-rabbit; donkey anti-chicken IgY, and donkey anti-goat. An anti-biotin conjugated horseradish peroxidase (HRP) tertiary antibody was used followed by diaminobenzidine (DAB, Dako, Carpinteria, CA) and counterstained with Gill’s Hematoxylin (Fisher Scientific, Fair Lawn, NJ). Controls were performed without primary antibodies for all the antibodies. Sections of rat liver and intestinal tissue were examined using Olympus BX51 light microscope with 10×, 20×, 40× and 60× objective. DP Manager Software was used to digitally record images.

Raghuvanshi S., Reed V., Blaner W.S, & Harrison E.H. (2015). Cellular localization of β-carotene 15,15′ oxygenase-1 (BCO1) and β-carotene 9′,10′ oxygenase-2 (BCO2) in rat liver and intestine. Archives of biochemistry and biophysics, 572, 19-27.

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Immunohistochemical Analysis of Tumor Samples

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A standard immunohistochemistry (IHC) protocol was used to analyze tumor samples harvested from the A549R xenograft experiment. Briefly, the sections (5 μm-thick) were first dewaxed and re-hydrated, and the endogenous peroxidase activity was blocked using 3% hydrogen peroxide. Antigen retrieval process was carried out in a pressure cooker where the slides were immersed in 10 mmol/L EDTA (pH 8.0) for 3 min, followed by blocking with 10% normal serum. The sections were the incubated with anti-Src (1:300), anti-MIF (1:200) and anti-CD155 (1:300) antibodies, followed by secondary antibody. The immunostaining was detected by an HRP Polymer Kit (Lab Vision, Fremont, CA, USA). DAB was used as the chromogenic substrate, and sections were counterstained with Gill’s hematoxylin (Fisher Scientific, NJ, USA). The study was approved by the MacKay Memorial Hospital (Approval number: 20160600011 16MMHIS073e) and followed standard institutional protocol for human research. The identity of patients was completely de-identified.

Huang W.C., Kuo K.T., Wang C.H., Yeh C.T, & Wang Y. (2019). Cisplatin resistant lung cancer cells promoted M2 polarization of tumor-associated macrophages via the Src/CD155/MIF functional pathway. Journal of Experimental & Clinical Cancer Research : CR, 38, 180.

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Immunohistochemical Tissue Analysis

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Five micrometre tumour sections were cut from embedded tissue using an HM 325 microtome (Thermo Scientific) and baked at 70°C for 30 min. Tissue sections were dewaxed in xylene followed by rehydration and antigen retrieval using citrate buffer (10 mM sodium citrate, 0.05% Tween 20), then washed and blocked with serum-free protein block (DAKO). Primary antibodies were diluted in antibody diluent (DAKO) and incubated overnight at 4°C then washed and stained with fluorescently conjugated secondary antibodies diluted 1:200 for 30 min at room temperature. For immunohistochemistry, endogenous peroxidase activity was blocked by incubation with 3% H₂O₂ in PBS, followed by primary and biotinylated secondary antibodies diluted 1:200. Antigen staining with VECTASTAIN Standard ABC Kit (Vector Laboratories) and Liquid DAB⁺ Substrate Chromagen System (DAKO) was performed, and slides were counterstained with Gill’s Hematoxylin (Fisher Scientific). Images were acquired on an Olympus BX43 microscope with a DP80 camera and analysed with manufacture’s software (Olympus).

Nywening T.M., Belt B.A., Cullinan D.R., Panni R.Z., Han B.J., Sanford D.E., Jacobs R.C., Ye J., Patel A.A., Gillanders W.E., Fields R.C., DeNardo D.G., Hawkins W.G., Goedegebuure P, & Linehan D.C. (2017). Targeting both tumour-associated CXCR2+ neutrophils and CCR2+ macrophages disrupts myeloid recruitment and improves chemotherapeutic responses in pancreatic ductal adenocarcinoma. Gut, 67(6), 1112-1123.

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Histological Analysis of IL-17A Expression in Lungs

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Lungs were fixed with 10% formaldehyde overnight and paraffin embedded. Blocks were sectioned at 500 μm intervals at a thickness of 5 μm and each section was deparaffinized, hydrated and stained with hematoxylin and eosin. Other sections were stained with rabbit anti-mouse IL-17A (Abcam, Cambridge, MA). For immunohistochemistry, sections were incubated with biotinylated secondary goat-IgG, ABC reagent (Vector Laboratories, Burlingame, CA), diaminobenzidine (DAB, Sigma-Aldrich) and Gill’s hematoxylin (Fisher Scientific, Kalamazoo, MI). For fluorescence microscopy, slides were incubated with Alexa Fluor (AF)-555-conjugated rat anti-mouse CD68 and AF488-conjugated rabbit anti-mouse IL-17A or AF-conjugated isotype control IgGs. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Images were visualized using a Olympus IX71inverted phase/epifluorescence microscope and digital CCD camera.

Hong J.Y., Chung Y., Steenrod J., Chen Q., Lei J., Comstock A.T., Goldsmith A.M., Bentley J.K., Sajjan U.S, & Hershenson M.B. (2014). Macrophage activation state determines the response to rhinovirus infection in a mouse model of allergic asthma. Respiratory Research, 15(1), 63.

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Histological Analysis of Mouse Lung

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For histology, mouse lungs were perfused through the pulmonary artery with PBS containing 5 mmol/L EDTA and fixed with 4% paraformaldehyde overnight. For immunohistochemistry, lung sections were stained with rabbit anti‐iLOV, then incubated with biotinylated secondary goat‐IgG, ABC reagent (Vector Laboratories, Burlingame, CA), diaminobenzidine (DAB, Sigma‐Aldrich, St. Louis, MO, USA), and Gill's hematoxylin (Fisher Scientific, Kalamazoo, MI). For fluorescence microscopy, slides were incubated with Alexa Fluor 488–conjugated iLOV, Alexa Fluor 555–conjugated mouse anti‐RV VP2/VP0, and Cy5–anti‐mouse CD68 (Biolegend, San Diego, CA). Nuclei were stained with 4′,6‐diamidino‐2‐phenylindole (DAPI). Images were acquired with a Zeiss ApoTome confocal microscope (Microscopy and Image Analysis Core, University of Michigan).

Han M., Rajput C., Hinde J.L., Wu Q., Lei J., Ishikawa T., Bentley J.K, & Hershenson M.B. (2018). Construction of a recombinant rhinovirus accommodating fluorescent marker expression. Influenza and Other Respiratory Viruses, 12(6), 717-727.

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Immunohistochemical Analysis of PDX Xenografts

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Standard immunohistochemistry (IHC) was used to analyze tissues from the PDX xenograft models and parent tumors. Briefly, after deparaffinization and rehydratation of 4-μm-thick tissue sections, endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 15 minutes at room temperature. After pressure cooking the slides in 10 mmol/L EDTA (pH 8.0) for 3 minutes, the sections were blocked with 10% normal serum from the secondary antibody species and then incubated for 1 hour as follows: monoclonal mouse anti-human hCD45 antibody (IR75161, DAKO), CD44 antibody (ab9524, Abcam), CD133 (130-090-422, miltenyi biotec), mouse anti-human CD20/CD3 double staining cocktail working solution (Kit-8803, Maxim Biotech, Fuzhou, China). Positive staining was detected using a Detection System HRP Polymer Kit (Lab Vision, Fremont, CA, USA). DAB was used as the chromogenic substrate, and sections were counterstained with Gill's hematoxylin (Fisher Scientific, Fair Lawn, NJ). The test specimens were then scored independently by two pathologists (Y.L. and Z.L.) in a blinded fashion.

Zhang L., Liu Y., Wang X., Tang Z., Li S., Hu Y., Zong X., Wu X., Bu Z., Wu A., Li Z., Li Z., Huang X., Jia L., Kang Q., Liu Y., Sutton D., Wang L., Luo L, & Ji J. (2015). The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice. Scientific Reports, 5, 9447.

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