Anti arp3

For imaging the nucleus, cells were incubated with 200 ng ml⁻¹ of Hoechst 33342 (Life Technologies) or 34580 (Invitrogen) for 30 min at 37 °C and 5% CO₂.
The following antibodies were used for immunoblotting: LmnA/C (H110, Santa Cruz), anti-ArpC4 and anti-sun2 (Abcam), and anti-actin (Millipore). Antibodies were used at 1:1,000 dilution.
For immunofluorescence, we used Alexa Fluor 594-coupled phalloidin (Invitrogen; 1:400), anti-LmnAC (N18, Santa Cruz (1:50) and clone 4C11 from Sigma Aldrich (1:2,000)), anti-Lamin B1 (ab16048, Abcam (1:500)) and anti-Arp3 (Abcam; 1:200); DAPI (4,6-diamidino-2-phenylindole) for DNA staining; and secondary antibodies anti-mouse-Alexa488 and anti-Goat-Alexa488 from Jackson ImmunoResearch Laboratories were used at 1:400. Slides were mounted with custom-made Moviol.
For lymphatic vessels visualization, anti-LYVE-1 (R&D System, 1/1,000) was used.
For drug treatment CK666, latrunculin A and blebbistatin were obtained from Tocris Bioscience, Y27632 from Calbiochem, nocodazole and DMSO from Sigma-Aldrich. For silencing, Smart pool ON-TARGETplus mouse LMNA siRNA, Arpc4 siRNA and Sun1 siRNA were purchased from Thermo Fisher Scientific. Micro-channels were coated with fibronectin (Sigma) or custom-made pLL(20)-g[3.5]-PEG(2)-Rhodamin.

Imatinib was purchased from Selleckchem company (Houston, USA). After being dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA), it was subpackaged in different concentrations and stored at -20℃. The following antibodies were used: anti-cofilin, anti-p-cofilin (Ser-3), and anti-GAPDH, which were all from Cell Signaling Technology (Beverly, MA, USA). Anti-Arp2, anti-Arp3 were from Abcam (Cambridge, UK).