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Fdft1

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FDFT1 is an enzyme that catalyzes the conversion of two molecules of farnesyl pyrophosphate into squalene, which is the first committed step in cholesterol biosynthesis. It plays a crucial role in the sterol biosynthesis pathway.

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Lab products found in correlation

Mln4924, by Selleck Chemicals (2 mentions) Src 3, by BD (2 mentions) Phospho s6k t389, by Cell Signaling Technology (2 mentions) Dhcr24, by Abcam (2 mentions) Dhcr7, by Abcam (2 mentions) Igf1r, by Santa Cruz Biotechnology (2 mentions) I bet762 i bet, by MedChemExpress (2 mentions) Phospho akt s473, by Cell Signaling Technology (2 mentions) Phospho akt t308, by Cell Signaling Technology (2 mentions) β tubulin, by Merck Group (2 mentions) Mg132, by Merck Group (2 mentions) Mk 2206, by Selleck Chemicals (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Raptor, by Cell Signaling Technology (2 mentions) Bortezomib, by Selleck Chemicals (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Cd63 h 193, by Santa Cruz Biotechnology (1 mentions) Alexa fluor conjugates, by Thermo Fisher Scientific (1 mentions) Eclipse ti a1r a1 confocal microscope, by Nikon (1 mentions) Filipin, by Merck Group (1 mentions)

4 protocols using fdft1

1

Collagen IV-coated Immunofluorescence Imaging

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Cells were grown directly on collagen IV-coated coverslips. Cells were fixed in 3.7% formalin, permeabilized using 0.1% Triton X-100, and treated with 0.1% SDS. They were blocked in 1% BSA and then incubated with primary antibody (MCM2, Cell Signaling, H3K9me2, Abcam, CD63 (H-193), Santa Cruz, FDFT1, Abcam), followed by the respective secondary antibody, Alexa Fluor conjugates (Invitrogen). Cells were mounted using hard-set mounting media containing DAPI (Vector Laboratories). Cells were stained with Filipin (Sigma) for cholesterol and Alexa 594-conjugated CTXB (Invitrogen) for lipid rafts. Immunofluorescence imaging was performed on a Nikon Eclipse Ti A1R-A1 confocal microscope.
S.a.l.o.n.y., Solé X., Alves C.P., Dey-Guha I., Ritsma L., Boukhali M., Lee J.H., Chowdhury J., Ross K.N., Haas W., Vasudevan S, & Ramaswamy S. (2015). AKT INHIBITION PROMOTES NON-AUTONOMOUS CANCER CELL SURVIVAL. Molecular cancer therapeutics, 15(1), 142-153.
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2

Antibody Panel for Protein Analysis

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The following antibodies were used: SPOP (ab137537; Abcam), SPOP (16750-1-AP; proteintech), BRD2 (A302-583A; Bethyl), BRD2 (ab139690; Abcam), BRD3 (A302-368A; Bethyl), BRD4 (ab128874; Abcam), BRD4 (A301-985A; Bethyl), Myc (9E10; Sigma-Aldrich), Myc (SC-40; Santa Cruz Biotechnology), FLAG (M2; Sigma), HA (MM5-101R; Convance), Actin (AC-74; Sigma-Aldrich), DEK (13962S; Cell Signaling Technology), ERG (SC-352; Santa Cruz Biotechnology), AR (SC-816; Santa Cruz Biotechnology), SRC-3 (611104; BD), phospho-AKT-S473 (9471; Cell Signaling Technology), phospho-AKT-T308 (9275S; Cell Signaling Technology), AKT (9272; Cell Signaling Technology), phospho-S6K-T389 (9205; Cell Signaling Technology), S6K (9202; Cell Signaling Technology), β-tubulin (T4026; Sigma-Aldrich), RAC1 (23A8; BD), FDFT1 (ab195046; Abcam), DHCR24 (ab137845; Abcam), DHCR7 (ab103296; Abcam), MVD (ab12906; Abcam), HER3 (12708S; Cell Signaling Technology), INSR (ab131238; Abcam), IGF1R (SC-9038; Santa Cruz Biotechnology), mTOR (2972, Cell Signaling Technology ), Raptor (24C12, Cell Signaling Technology). MG132 and cycloheximide were purchased from Sigma-Aldrich, MLN4924, Bortezomib and MK2206 were purchased from Selleckchem. JQ1 was kindly provided by Dr. James Bradner and purchased from Sigma-Aldrich. i-BET762 (i-BET) was purchased from MedchemExpress. GDC-0068 was purchased from Calbiochem.
Zhang P., Wang D., Zhao Y., Ren S., Gao K., Ye Z., Wang S., Pan C.W., Zhu Y., Yan Y., Yang Y., Wu D., He Y., Zhang J., Lu D., Liu X., Yu L., Zhao S., Li Y., Lin D., Wang Y., Wang L., Chen Y., Sun Y., Wang C, & Huang H. (2017). Intrinsic BET inhibitor resistance in prostate cancer caused by SPOP mutation-mediated BET protein stabilization. Nature medicine, 23(9), 1055-1062.
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3

Antibody Panel for Protein Analysis

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The following antibodies were used: SPOP (ab137537; Abcam), SPOP (16750-1-AP; proteintech), BRD2 (A302-583A; Bethyl), BRD2 (ab139690; Abcam), BRD3 (A302-368A; Bethyl), BRD4 (ab128874; Abcam), BRD4 (A301-985A; Bethyl), Myc (9E10; Sigma-Aldrich), Myc (SC-40; Santa Cruz Biotechnology), FLAG (M2; Sigma), HA (MM5-101R; Convance), Actin (AC-74; Sigma-Aldrich), DEK (13962S; Cell Signaling Technology), ERG (SC-352; Santa Cruz Biotechnology), AR (SC-816; Santa Cruz Biotechnology), SRC-3 (611104; BD), phospho-AKT-S473 (9471; Cell Signaling Technology), phospho-AKT-T308 (9275S; Cell Signaling Technology), AKT (9272; Cell Signaling Technology), phospho-S6K-T389 (9205; Cell Signaling Technology), S6K (9202; Cell Signaling Technology), β-tubulin (T4026; Sigma-Aldrich), RAC1 (23A8; BD), FDFT1 (ab195046; Abcam), DHCR24 (ab137845; Abcam), DHCR7 (ab103296; Abcam), MVD (ab12906; Abcam), HER3 (12708S; Cell Signaling Technology), INSR (ab131238; Abcam), IGF1R (SC-9038; Santa Cruz Biotechnology), mTOR (2972, Cell Signaling Technology ), Raptor (24C12, Cell Signaling Technology). MG132 and cycloheximide were purchased from Sigma-Aldrich, MLN4924, Bortezomib and MK2206 were purchased from Selleckchem. JQ1 was kindly provided by Dr. James Bradner and purchased from Sigma-Aldrich. i-BET762 (i-BET) was purchased from MedchemExpress. GDC-0068 was purchased from Calbiochem.
Zhang P., Wang D., Zhao Y., Ren S., Gao K., Ye Z., Wang S., Pan C.W., Zhu Y., Yan Y., Yang Y., Wu D., He Y., Zhang J., Lu D., Liu X., Yu L., Zhao S., Li Y., Lin D., Wang Y., Wang L., Chen Y., Sun Y., Wang C, & Huang H. (2017). Intrinsic BET inhibitor resistance in prostate cancer caused by SPOP mutation-mediated BET protein stabilization. Nature medicine, 23(9), 1055-1062.
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4

Phosphorylation of SR Proteins Regulates Cell Signaling

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Cell Signaling Technology custom developed the pSRPK2(S494), pSRPK2(S497), and pSRPK2(S494/S497) antibodies for this study. Antibodies were obtained from following sources: SRPK1 and SRPK2 from BD Biosciences; ACLY, FASN, SCD1, S6, pS6(S235/S236), pS6(S240/S244), S6K1, pS6K1(T389), TSC2, and V5 from Cell Signaling Technology; ACSS2 and GAPDH from Sigma-Aldrich; FDFT1 from Abcam; SRSF1, ACTIN, GST, LAMIN A/C, and SREBP1 from Santa Cruz; HA from Covance; U1-70k from Synaptic Systems. Monoclonal antibody for the phosphorylated SR proteins was generated from the mAb104 hybridoma cell line (ATCC) (Figures 3B and 3H) or purchased from invitrogen (mAB1H4) (Figure 5G). Reagents were obtained from following sources: Insulin, rapamycin, PF4708671, and actinomycin D from Sigma-Aldrich; Torin1 from Tocris Bioscience; rapamycin from Calbiochem; MK2206 from Cayman Chemical; SRPIN340 from Selleck Chemicals and Milstein Chemistry Core Facility (Weill Cornell Medicine).
Lee G., Zheng Y., Cho S., Jang C., England C., Dempsey J.M., Yu Y., Liu X., He L., Cavaliere P.M., Chavez A., Zhang E., Isik M., Couvillon A., Dephoure N.E., Blackwell T.K., Yu J.J., Rabinowitz J.D., Cantley L.C, & Blenis J. (2017). Post-transcriptional regulation of de novo lipogenesis by mTORC1-S6K1-SRPK2 signaling. Cell, 171(7), 1545-1558.e18.
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