Anti ly6g ly6c

Manufactured by Thermo Fisher Scientific

Anti-Ly6G/Ly6C is a laboratory reagent used for the detection and analysis of Ly6G and Ly6C proteins in various biological samples. It is a monoclonal antibody that specifically binds to these two protein targets, enabling their identification and quantification through various immunoassay techniques.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti-Ly6G (36 citations) Anti-Ly6C (23 citations)

5 protocols using anti ly6g ly6c

Immunohistochemical Analysis of Colorectal Cancer

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colorectal cancer tissues were obtained from US Biomax. The staining procedure for paraffin‐embedded mouse and human tissues were as previously described (Mu et al, 2018 (link)). Tissue staining was semi‐quantitatively scored on a 0–9 scale by multiplying the score for the proportion of positively stained tumor cells (0, < 10%; 1, 10–25%; 2, 26–50%; 3, > 50%) by the staining intensity score (0–3) as previously defined (Wang et al, 2021 (link)). For the comparison of low/high PCIF1 expression and patient survival, overall survival was defined as the time between date of diagnosis and death. Primary antibodies used in IHC include anti‐PCIF1 (Invitrogen, PA5‐61996), anti‐FOS (Abcam, ab222699), anti‐Ki‐67 (Cell Signaling Technology, 12202T), anti‐Ly‐6G/Ly6C (Invitrogen, 14‐5931‐81), and anti‐NK1.1 (Invitrogen, MA1‐70100).

Wang L., Wu L., Zhu Z., Zhang Q., Li W., Gonzalez G.M., Wang Y, & Rana T.M. (2022). Role of PCIF1‐mediated 5′‐cap N6‐methyladeonsine mRNA methylation in colorectal cancer and anti‐PD‐1 immunotherapy. The EMBO Journal, 42(2), e111673.

+ Open protocol

+ Expand

Murine Bone Marrow Cell Isolation and Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow was isolated from tibia and femur of mice and erythrocytes were lysed. To sort pre-progenitor cells staining for linage markers anti-CD3e (Cat # 45-0031-80, eBioscience, 1:50), anti-GR1 (Cat # 108428, BioLegend, 1:400), anti-Mac-1 (Cat # 101228, BioLegend, 1:400), anti-CD4 (Cat # 101228, BioLegend, 1:200), anti-CD8a (Cat # 100734, BioLegend, 1:100), anti-TCRb (Cat # 109228, BioLegend, 1:100), anti-NK1.1 (Cat # 45-5941-82, eBioscience, 1:100), anti-B220 (Cat # 103236, BioLegend, 1:100) and anti-CD19 (Cat # 115534, BioLegend, 1:200) was performed. Additionally anti-CD16/32 (Cat # 101305, BioLegend, 1:200), anti-CD41 (Cat # 133906, BioLegend, 1:100), anti-Sca-1 (Cat # 108114, BioLegend, 1:200), anti-c-kit (Cat # 47-1171-82, Invitrogen, 1:50), anti-CD105 (Cat # 120412, BioLegend, 1:50) and anti-CD150 (Cat # 115910, BioLegend, 1:400) were stained to further classify cells. To sort immature granulocytes, mature granulocytes and monocytes anti-Ly6G/Ly6C (Cat # 17-5931-82, Invitrogen, 1:100), anti-CD11b (Cat # 25-0112-82, eBioscience, 1:100) and anti-CD115 (Cat # 11-1031-85, eBioscience, 1:100) were used. Gating strategy was performed as shown in Supplementary Fig 5.

Lang J., Bohn P., Bhat H., Jastrow H., Walkenfort B., Cansiz F., Fink J., Bauer M., Olszewski D., Ramos-Nascimento A., Duhan V., Friedrich S.K., Becker K.A., Krawczyk A., Edwards M.J., Burchert A., Huber M., Friebus-Kardash J., Göthert J.R., Hardt C., Probst H.C., Schumacher F., Köhrer K., Kleuser B., Babiychuk E.B., Sodeik B., Seibel J., Greber U.F., Lang P.A., Gulbins E, & Lang K.S. (2020). Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. Nature Communications, 11, 1338.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

LNs and spleen tissues were prepared using mechanical dissociation of minced tissue to obtain single cell suspensions. The following antibodies were used for flow cytometry: anti-Ly6G/Ly6C (Cat #58-5931-82), anti-Cd11b (Cat #12-0112-82), anti-CD86 (Cat #11-0862-82), anti-F4/80 (Cat #17-4801-82), anti-MHCII (Cat #13-5321-82), and anti-CD11c (Cat #11-0116-42) all from eBioscience, San Diego, CA) and anti-CD206 (clone MR5D3; BioRad). After being stained with a standard protocol, all events were analyzed with FlowJo software (FlowJo, LLC, Ashland, OR) and frequencies among live cells were obtained.

Cheng R., Billet S., Liu C., Haldar S., Choudhury D., Tripathi M., Hav M., Merchant A., Hu T., Huang H., Zhou H, & Bhowmick N.A. (2019). Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells. Oncogene, 39(7), 1543-1556.

+ Open protocol

+ Expand

Profiling Prostate Cell Populations by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cells isolated from a prostate were incubated with an anti-CD16/32 antibody (BD Pharmingen; 1:50) for 15 min on ice and with an antibody cocktail for 15 min on ice. The antibody cocktail included anti-CD326 (EpCAM) [BioLegend; reference: 118216; conjugation: phycoerythrin (PE)/Cy7], anti-CD45 (BioLegend; reference: 103128; conjugation: Alexa Fluor 700), anti-CD11b [eBioscience; reference: 45-0112-82; conjugation: peridinin chlorophyll protein (PerCP)–Cy5.5], and anti–Ly-6G/Ly-6C [eBioscience; reference: 11-5931-82; conjugation: fluorescein isothiocyanate (FITC)]. All antibodies were diluted 1:200 in DMEM [glucose (4.5 g/liter), 1% P/S, and 2% bovine serum albumin (BSA), without phenol red]. Samples were analyzed by a BD LSR II flow cytometer and the FlowJo software. The antibody cocktail enabled the quantification of epithelial cells (EpCAM⁺; CD45⁻), leukocytes (CD45⁺), stromal cells (EpCAM⁻CD45⁻), and MDSCs [CD45⁺CD11b⁺Ly-6G/Ly-6C (Gr-1)⁺].

Abu el Maaty M.A., Grelet E., Keime C., Rerra A.I., Gantzer J., Emprou C., Terzic J., Lutzing R., Bornert J.M., Laverny G, & Metzger D. (2021). Single-cell analyses unravel cell type–specific responses to a vitamin D analog in prostatic precancerous lesions. Science Advances, 7(31), eabg5982.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dissociated prostates were incubated with anti-CD16/32 antibody (BD Pharmingen; 1:50) for 15 min on ice. To quantify total leukocytes (CD45⁺) and MDSCs (CD11b⁺Gr-1⁺), cells were incubated with anti-CD45 (BioLegend; ref. 103128; conjugation: Alexa Fluor 700), anti-CD11b (eBioscience; ref. 45-0112-82; conjugation: PerCP-Cy5.5), and anti–Ly-6G/Ly-6C (Gr-1) [eBioscience; ref. 11-5931-82; conjugation: fluorescein isothiocyanate (FITC)] antibodies for 15 min on ice. All antibodies were diluted 1:200 in DMEM [glucose (4.5 g/liter), 1% P/S, and 2% bovine serum albumin, without phenol red].
To quantify CD8⁺ T cells (CD3⁺CD8⁺) and NK cells (CD3⁻CD49b⁺NK1.1⁺), samples were incubated with anti-CD3ε (BioLegend; ref. 100328; conjugation: PerCP/Cy5.5; dilution 1:50), anti-CD8a (eBioscience; ref. 56-0081-80; conjugation: Alexa Fluor 700; dilution: 1:100), anti-CD49b (eBioscience; ref. 11-5971-82; conjugation: FITC; dilution: 1:100), and anti-NK1.1 (eBioscience; ref. 25-5941-82; conjugation: PE-Cyanine7; dilution: 1:100) antibodies for 15 min on ice. Cells were analyzed using a BD LSR II flow cytometer and the FlowJo software.

Abu el Maaty M.A., Terzic J., Keime C., Rovito D., Lutzing R., Yanushko D., Parisotto M., Grelet E., Namer I.J., Lindner V., Laverny G, & Metzger D. (2022). Hypoxia-mediated stabilization of HIF1A in prostatic intraepithelial neoplasia promotes cell plasticity and malignant progression. Science Advances, 8(29), eabo2295.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!