Alexa fluor 488 donkey anti mouse conjugated secondary antibodies

The engineered cell sheets were fixed in 10% neutral buffered formalin and embedded in paraffin. The tissue sections were used for immunofluorescent staining with CD19 (Cat # NBP2-25196), CD73 (Cat # NBP2-25237) (NovusBIo, CO, United States), CD29 (Cat # ab134179), HLA-A (Cat # ab52922), HLA-DR (Cat # ab92511) (Abcam, MA, United States), and Oct3/4 (Cat # NB-100-2379SS) (Novus Biologicals LLC., Littleton, CO, United States). Alexa Fluor 488 donkey anti-rabbit conjugated secondary antibodies and Alexa Fluor 488 donkey anti-mouse conjugated secondary antibodies (Invitrogen, Carlsbad, CA, United States) were used. Propidium iodide (Invitrogen, Carlsbad, CA, United States) was used to stain nuclear DNA. An EVOS M5000 microscope was used to analyze the slides (Invitrogen, Carlsbad, CA, United States).

Engineered cell sheets were fixed in 10% neutral buffered formalin and embedded in paraffin. Tissue sections were used for immunofluorescent staining with CD19, CD73 (NovusBIo, CO, USA), CD29, HLA-A, HLA-DR (Abcam, MA, USA), Oct3/4 (Novus Biologicals LLC., Littleton, CO, USA). Alexa Fluor 488 donkey anti-rabbit conjugated secondary antibodies and Alexa Fluor 488 donkey anti-mouse conjugated secondary antibodies (Invitrogen, Carlsbad, CA, USA) were used. Propidium iodide (Invitrogen, Carlsbad, CA, USA) was used to stain nuclear DNA. A EVOS M5000 microscope was used to analyze the slides (Invitrogen, Carlsbad, CA, USA).