Recombinant rat il 1β 501 rl

Manufactured by R&D Systems

Sourced in United States

Recombinant rat IL-1β (501-RL) is a laboratory product manufactured by R&D Systems. It is a purified recombinant protein that represents the mature form of rat Interleukin-1 beta.

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Lab products found in correlation

Ab36861, by Abcam (2 mentions) Ab41037, by Abcam (1 mentions) Gapdh af7021, by Affinity Biosciences (1 mentions) Laminb1 12987 1 ap, by Proteintech (1 mentions) Fetal bovine serum fbs 10099 141, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem f12, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) A02802 1, by Boster Bio (1 mentions) Ab188570, by Abcam (1 mentions) Phosphorylated erk p erk, by Cell Signaling Technology (1 mentions) Phosphorylated iκbα p iκbα, by Cell Signaling Technology (1 mentions) Mmp13 18165 1 ap, by Proteintech (1 mentions) Phosphorylated jnk p jnk, by Cell Signaling Technology (1 mentions) Ab15323, by Abcam (1 mentions)

Spelling variants of this product

IL-1β (1098 citations) Recombinant IL-1β (27 citations) Recombinant rat IL-1β (18 citations) Rat IL-1β (8 citations) Recombinant rat IL-1β (501-RL-010) (3 citations)

2 protocols using recombinant rat il 1β 501 rl

Chondroprotective Effects of Q3GA

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Q3GA (molecular formula: C₂₁H₁₈O₁₃, MW: 478.36, CAS No. 22688-79-5) was obtained from Chengdu Alfa Biotechnology Co. Ltd. (Sichuan, China; ABL1913). Recombinant rat IL-1β (501-RL) and recombinant human IL-1β (201-RL) were purchased from R&D Systems (Minneapolis, USA). SW1353 cells were purchased from the Cell Bank of Shanghai Institute of Biochemistry and Cell Biology. Dulbecco's modified Eagle's medium/Nutrient Mixture F-12 (DMEM/F-12; 11320033), Dulbecco's modified Eagle's medium (DMEM; C11995500BT) and fetal bovine serum (FBS; 10099141) were purchased from Gibco (NY, USA). Primary antibodies against ACAN (ab36861) and ADAMTS5 (ab41037) were purchased from Abcam (Cambridge, UK); iNOS (18985-1-AP), COX2 (12375-1-AP), p65 (66535-1-Ig), Nrf2 (16396-1-AP), HO-1 (10701-1-AP), and LaminB1 (12987-1-AP) from Proteintech (Wuhan, China); p-p65 (WL02169) from Wanleibio (Shenyang, China); Col2a1(AF0135) and MMP13 (AF5355), MMP3 (AF0217), IκBα (AF5002) and GAPDH (AF7021) from Affinity (Melbourne, USA). The secondary antibodies goat anti-rabbit IgG (10004301) and goat anti-mouse IgG (10004302) were purchased from MyBioScience (Nanjing, China).

Mao H., Feng Y., Feng J., Yusufu Y., Sun M., Yang L, & Jiang Q. (2024). Quercetin-3-O-β-D-glucuronide attenuates osteoarthritis by inhibiting cartilage extracellular matrix degradation and inflammation. Journal of Orthopaedic Translation, 45, 236-246.

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Dioscin Modulates Inflammatory Pathways

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Recombinant rat IL-1β (501-RL) was purchased from R&D Systems (Minneapolis, MN, USA). Dioscin (N1723) was supplied by ApexBio Technology (Houston, TX, USA). Dioscin was dissolved in dimethyl sulfoxide (DMSO) for in vitro experiments. Dulbecco’s modified Eagle’s medium (DMEM/F12) and fetal bovine serum (FBS) were procured from Gibco (Grand Island, NY, USA). Antibodies specific for iNOS (ab15323), aggrecan (ab36861), and collagen II (ab188570) were obtained from Abcam (Cambridge, UK). Antibodies specific for P65 (#8242), phosphorylated P65 (P-P65) (#3033), IκBα (#4814), phosphorylated-IκBα (P-IκBα) (#2859), IKKβ (#8943), phosphorylated-IKKα/β (P-IKKα/β) (#2697), P38 (#8690), phosphorylated-p38 (p-p38) (#4511), ERK (#4695), phosphorylated-ERK (P-ERK) (#4370), JNK (#9252), and phosphorylated-JNK (P-JNK) (#4668) were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies specific for MMP1 (10,371–2-AP), MMP3 (17,873–1-AP), and MMP13 (18,165–1-AP) were supplied by Proteintech Group (Wuhan, China). Antibodies against GAPDH (BM1623) and ADAMTS5 (A02802-1) were procured from Boster (Wuhan, China).

Ding Q., Zhang R., Sheng G., Wang T., Jing S., Ma T., Wang S., Zhao H., Wu H, & Li W. (2023). Dioscin alleviates the progression of osteoarthritis: an in vitro and in vivo study. Journal of Inflammation (London, England), 20, 14.

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