HPLC grade methanol (MeOH) and ethyl acetate (EtOAc) were purchased from Fisher Chemicals, Reinach, Switzerland, LC-MS grade water, acetonitrile (ACN), and formic acid were purchased from Fisher Chemicals, Reinach, Switzerland, Dimethyl Sulfoxide (DMSO) molecular biology grade was purchased from Sigma, St Louis, United States.
Molecular biology grade dimethyl sulfoxide dmso
Manufactured by Merck Group
Sourced in United States
Molecular biology grade dimethyl sulfoxide (DMSO) is a clear, colorless liquid commonly used as a solvent in various laboratory applications. It has a high boiling point and is miscible with water and many organic solvents. DMSO is widely used in molecular biology research due to its ability to facilitate the dissolution and stabilization of biomolecules, including proteins, nucleic acids, and small molecules.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (2 mentions)
Tris buffer, by Merck Group (2 mentions)
Lc ms grade water, by Thermo Fisher Scientific (1 mentions)
Hplc grade methanol meoh, by Thermo Fisher Scientific (1 mentions)
Reagent grade formic acid, by Merck Group (1 mentions)
Remdesivir, by Selleck Chemicals (1 mentions)
Optima hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Millipore system, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions)
Mtt reagent, by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Normal melting point agarose, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Kemika (1 mentions)
Sodium chloride (nacl), by Kemika (1 mentions)
Ethanol, by Kemika (1 mentions)
Czapek yeast agar, by Thermo Fisher Scientific (1 mentions)
Low melting point agarose lmp, by Merck Group (1 mentions)
Penicillin, by Lonza (1 mentions)
7 protocols using molecular biology grade dimethyl sulfoxide dmso
1
Solvent Preparation for LC-MS
2
Quantitative Analysis of Remdesivir
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Remdesivir (>99% pure) was purchased from Selleck Chemicals (Houston, TX), and the internal standard, Remdesivir-2H5 (>99% pure), was purchased from MedChemExpress (Monmouth, NJ). Analyte and internal standard chemical structures are provided in Fig. 1 .![]()
Structures of the analyte Remdesivir
3
Cytotoxicity Assay Protocol
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Acetonitrile, ethyl acetate, formic acid and methanol were purchased from Kefo (Sisak, Croatia); Czapek Yeast Agar (CYA) was purchased from Oxoid (Hampshire, UK). Medium RPMI 1640, fetal bovine serum (FBS), phosphate buffered saline (PBS; without Ca2+ and Mg2+), trypsin-EDTA, antibiotics penicillin and streptomycin were from Lonza (Basel, Switzerland). The MTT reagent [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], acridine orange, ethidium bromide, normal melting point agarose (NMP), low melting point agarose (LMP), phorbol 12-myristate 13-acetate (PMA), Triton X-100 and Tris buffer were purchased from Sigma-Aldrich (Deisenhofen, Germany) and molecular biology grade dimethyl sulfoxide (DMSO), used for dissolving the extracts and for the control treatment, was purchased from Sigma-Aldrich (Deisenhofen, Germany); technical grade DMSO, used for dissolving the formazane, ethanol, sodium chloride, disodium-EDTA and sodium hydroxide were from Kemika (Zagreb, Croatia).
4
CDK Kinase Assay and Molecular Docking of Natural Products
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The ADP-Glo kinase assay kit and CDK enzymes
were purchased from Promega Corporation. Molecular biology-grade dimethyl
sulfoxide (DMSO), deionized water, and Tris buffer were purchased
from Sigma-Aldrich. The group’s NP library (n = 101) used herein is a collection of pure NPs isolated by our group
under several projects, along with a few NPs commercially procured
from different vendors. The source of top actives/hits is provided
here. Rohitukine,28 (link) boeravinone B,29 (link) pongol and structural analogues,30 (link) and helminthosporin20 (link) were obtained from our phytochemistry research. Other NPs, viz.,
rutin (TCI, R0035), chrysin (TCI, C1652), bergenin (TCI, B4349), morin
(TCI, P0041), quercetin (TCI, P0042), baicalein (TCI, T2721), and
fisetin (TCI, T0121), were sourced from commercial vendors. Luminescence
readings in the CDK kinase assay were recorded using a Tecan Infinite
M Plex multimode reader, and the dose–response curve was generated
by using GraphPad Prism. Molecular docking was carried out using Schrodinger
molecular modeling software.
were purchased from Promega Corporation. Molecular biology-grade dimethyl
sulfoxide (DMSO), deionized water, and Tris buffer were purchased
from Sigma-Aldrich. The group’s NP library (n = 101) used herein is a collection of pure NPs isolated by our group
under several projects, along with a few NPs commercially procured
from different vendors. The source of top actives/hits is provided
here. Rohitukine,28 (link) boeravinone B,29 (link) pongol and structural analogues,30 (link) and helminthosporin20 (link) were obtained from our phytochemistry research. Other NPs, viz.,
rutin (TCI, R0035), chrysin (TCI, C1652), bergenin (TCI, B4349), morin
(TCI, P0041), quercetin (TCI, P0042), baicalein (TCI, T2721), and
fisetin (TCI, T0121), were sourced from commercial vendors. Luminescence
readings in the CDK kinase assay were recorded using a Tecan Infinite
M Plex multimode reader, and the dose–response curve was generated
by using GraphPad Prism. Molecular docking was carried out using Schrodinger
molecular modeling software.
5
Isolation and Characterization of Bioactive Compounds
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Molecular biology-grade dimethyl sulfoxide (DMSO) was procured from Sigma-Aldrich (St. Louis, MO, USA). Platelet-derived growth factor-BB (PDGF-BB) (Catalog number: 100-14B) was obtained from PeproTech (Rehovot, Israel). 4' ,6-Diamidino-2-phenylindole dihydrochloride (DAPI) was purchased from Invitrogen™ (Waltham, MA, USA). Silica gel 60 (230-400 mesh) was sourced from Acme Synthetic Chemicals (Mumbai, India). Dragon blood, a resin obtained from the fruit of Daemonorops draco (Wu et al. 2019) , and catechu, a dried, evaporated decoction of Uncaria gambir Roxb (Ho et al. 2022) , have been previously documented (Ching et al. 2023; Ho et al. 2022) .
6
Synthesis and analysis of neurochemicals
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Chemical synthesis: trans-caffeic acid, trans-ferulic acid, 3,4-dihydroxyhydrocinnamic acid, phenethylamine, tryptamine, tyramine, dopamine hydrochloride, and N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride were purchased from Sigma–Aldrich (St. Louis, MO, USA). Triethylamine, 3,4-dimethoxyphenethylamine, LC-MS grade methanol, acetonitrile, water, and formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Dimethylformamide (DMF), ethyl acetate, and hexane were purchased from Pharmco-AAPER (Brookfield, CT, USA).
In vitro studies: Gibco Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and streptomycin/penicillin solution were purchased from Thermo Fisher Scientific (Hagerstown, MD, USA). Dimethyl sulfoxide-d6 was purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA) sulfanilamide, naphthylethylenediamine dihydrochloride, molecular biology grade dimethyl sulfoxide (DMSO) and lipopolysaccharide (LPS) (Escherichia coli O127: E8) were purchased from Sigma–Aldrich (St. Louis, MO, USA).
In vitro studies: Gibco Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and streptomycin/penicillin solution were purchased from Thermo Fisher Scientific (Hagerstown, MD, USA). Dimethyl sulfoxide-d6 was purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA) sulfanilamide, naphthylethylenediamine dihydrochloride, molecular biology grade dimethyl sulfoxide (DMSO) and lipopolysaccharide (LPS) (Escherichia coli O127: E8) were purchased from Sigma–Aldrich (St. Louis, MO, USA).
7
Berberine-Mediated Apoptosis Pathway
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Berberine (>98% pure) and molecular biology-grade dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) and all chemicals used for cell culture were purchased from HyClone (Logan, UT, USA). Penicillin, streptomycin, trypsin, and ethylene diamine tetraacetic acid (EDTA) were also purchased from Sigma-Aldrich. Commonly used chemical reagents in this study were mostly procured from China. Rabbit anti-human Bax, Bcl-2, caspase-3, caspase-9, cyto-C, poly ADP ribose polymerase (PARP), and Ezrin were procured from Cell Signal Technology (Danvers, MA, USA). Rabbit antihuman β-actin antibody and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies used in this study were purchased from KPL (Gaithersburg, MD, USA). The reagents used for polymerase chain reaction (PCR) and western blot analysis were procured from Tiangen Biotech (Beijing, China) and Beyotime (Jiangsu, China), respectively. Unless otherwise mentioned, control treatment involved the treatment of cells with an equal volume of complete medium.
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