In vivo imaging system ivis 50

The therapeutic efficacy was assessed by the bioluminescent signal acquired from the In Vivo Imaging System (IVIS 50, Perkin Elmer Inc., Waltham, MA, USA) or caliper measurement of tumor volume. Tumor volume was calculated by the formula as (width² x length)/2. the quantified results were normalized to the day treatment begun. The survival was recorded since the first mice died from the treatment or the disease up to 60 days. The record was then calculated by MedCalc software with the log-rank examination for the significant difference. The Kaplan-Meier plot was used for demonstration of survival with 95% confidence interval.

After administration of ¹⁸⁸Re-liposome, CLI was performed to acquire signals using the In Vivo Imaging System (IVIS 50, Perkin Elmer Inc., Waltham, MA, USA). For evaluation of therapeutic efficacy, the tumor viability and growth rate were assessed using the luciferase based reporter gene imaging and caliper measurement, respectively. The tumor volume was determined by the formula: (width² × length)/2 after caliper measurement. For survival analysis, the end point of each datum was established when tumor volume reached 1000 mm³ by caliper measurement, or when the body weight reduced over 25% from the first day of treatment.