Sputum was induced by inhalation of hypertonic saline and treated with dithioerythritol (Sigma-Aldrich Co., St Louis, MO, USA) as recommended by the European Respiratory Society Task Force and described earlier in detail.9 (link),29 (link),30 (link) The supernatant was frozen at −80°C for biochemical analyses. Cell viability was studied with Trypan blue in a Burker chamber.31 (link) Cytocentrifuge preparations were made by Cytospin (Shandon Cytospin 3) and centrifuged at 450 rpm for 6 minutes. The slides were stained with May–Grunwald–Giemsa solution (EMD Millipore, Billerica, MA, USA) for cell differential counts. Detailed cell profiles were assessed based on 400 cells counted from each slide. Only the representative samples with <70% of squamous epithelial cells were accepted for the assessments.9 (link),31 (link) The slides were frozen at −20°C. Induction of the sputum samples in the Japanese cohort has been described earlier.32 (link)
May grunwald giemsa solution
Manufactured by Merck Group
Sourced in United States, Germany
May-Grunwald-Giemsa solution is a staining reagent commonly used in hematology and cytology laboratories. It is a mixture of methylene blue, eosin, and azure dyes that is used to stain blood smears and other cellular preparations for microscopic examination. The solution is used to differentiate and identify various cell types based on their staining characteristics.
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Lab products found in correlation
3 protocols using may grunwald giemsa solution
1
Sputum Induction and Cell Analysis
2
Cell Morphology Analysis via Light Microscopy
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Morphological changes were examined in cell smears using light microscopy of cytospin preparations stained with May-Grunwald-Giemsa solution (Merck, Darmstadt, Germany).
3
BAL Cell Analysis and Cytokine Quantification
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A total of 100,000 viable BAL cells was cytocentrifuged onto slides by a Cytospin 4 (Thermo Electron) and stained with May-Grunwald-Giemsa solution (Merck). Two hundred leukocytes were counted on each slide. The cell types were identified using morphological criteria. The concentration of IL-17A, IL-1β, and IL-6 in the BAL fluid was assessed with a CBA Mouse Enhanced Sensitivity Flex Set System (BD Biosciences).
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