Cell cycle: PBS washed 3 times, trypsin digested for 3 min, fixed in 70% ethanol at 4℃ for 30 min, and then incubated in 500 μl of propidium iodide staining solution (PI) (Beyotime) at 37 ℃ for 30 min. Apoptosis: PBS washed 3 times, trypsin digested for 3 min, and then annexin-FITC reagent (Beyotime) was added in order, and incubated for 20 min, protected from light. Cell cycle and apoptosis were detected by flow cytometry (BD Biosciences, USA), and the results were analyzed using flowJo software.
Propidium iodide staining solution pi
Manufactured by Beyotime
Propidium iodide (PI) staining solution is a fluorescent dye used for nuclear staining in cell biology applications. It selectively binds to the DNA of cells and emits a red fluorescence when excited by a light source. This solution can be used to stain and identify cells, particularly during cell cycle analysis and cell death studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using propidium iodide staining solution pi
1
Cell Cycle and Apoptosis Analysis
2
Cell Cycle and Apoptosis Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell cycle and apoptosis were measured using flow cytometry. For the cell cycle, the cells were washed twice with PBS and digested with trypsin, 70% ethanol for 4 h at 24° C, it was subsequently incubated in 500 µl of configured propidium iodide staining solution (PI) (Beyotime) at 37° C for 30 min. With regard to cell apoptosis, cells were washed twice with PBS and digested with trypsin, add the Annexin-FITC detection reagent (Beyotime) in turn.Then the cells were detected using a FACS flow cytometer (BD Biosciences, CA, USA), and analyzed the results using the Flow Jo software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!