Human colorectal adenocarcinoma-derived cell line LoVo, of which some loci were different from those of an original LoVo (ATCC: CCL-29), was kindly provided by Kirin corporation. Human colorectal adenocarcinoma-derived cell line HCT116, HT29, and DLD-1, human non-small cell lung cancer (NSCLC) adenocarcinoma-derived cell line A549, and human prostate cancer-derived cell line LNCaP were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). LoVo, HCT116, HT29, DLD-1, and LNCaP cells were maintained in Roswell Park Memorial Institute (RPMI) medium 1640 (RPMI1640; Nissui Pharmaceutical, Tokyo, Japan) containing 10% fetal bovine serum (FBS; JRH Biosciences), 100 U/mL of penicillin G (Sigma #13752), and 0.1 mg/mL of kanamycin (Sigma #K1377). A549 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Nissui Pharmaceutical) supplemented with 10% FBS, 100 U/mL of penicillin G, and 0.1 mg/mL of kanamycin. All cell lines were maintained at 37°C in a 5% CO2–95% air atmosphere. Unfortunately, we conducted a study using Mycoplasma-infected cells in some experiments.
Dld 1
Manufactured by Nissui Pharmaceutical
Sourced in Japan
The DLD-1 is a laboratory equipment designed for biological research applications. It is a cell line derived from human colorectal adenocarcinoma. The core function of the DLD-1 is to serve as a model system for in vitro studies in the field of cancer biology and drug development.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Nissui Pharmaceutical (2 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Penicillin g, by Merck Group (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Dld 1, by American Type Culture Collection (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Kanamycin, by Nissui Pharmaceutical (1 mentions)
Lncap, by Nissui Pharmaceutical (1 mentions)
Rpmi 1640 medium, by Fujifilm (1 mentions)
Balb c mice, by Japan SLC (1 mentions)
Balb c nu nu mice, by Japan SLC (1 mentions)
2 protocols using dld 1
1
Cultivation of Common Cancer Cell Lines
2
Murine Colon Cancer Cell Lines
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Five-week-old male BALB/c mice and BALB/c nu/nu mice were purchased from Japan SLC (Shizuoka, Japan). The experimental animals were allowed free access to water and mouse chow and were housed under controlled environmental conditions (constant temperature, humidity, and 12-hour dark–light cycle). All animal experiments were evaluated and approved by the Animal and Ethics Review Committee of the Tokushima University and conducted in accordance with the guidelines of animal welfare and rights in Japan.
Colon 26 (C26) murine colorectal carcinoma cell line was purchased from Cell Resource Center for Biomedical Research (Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan). A human colon carcinoma cell line, DLD-1 and DLD-1/FU (5-FU resistance), was kindly provided by Taiho Pharmaceutical.23 (link) Each cell line was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Nissui Pharmaceutical, Tokyo, Japan) and RPMI-1640 medium (Wako Pure Chemical) supplemented with 10% heat-inactivated fetal bovine serum (Japan Bioserum, Hiroshima, Japan), 100 units/mL penicillin, and 100 μg/mL streptomycin (ICN Biomedical, Aurora, OH, USA) in a 5% CO2/air incubator at 37°C, respectively.
Colon 26 (C26) murine colorectal carcinoma cell line was purchased from Cell Resource Center for Biomedical Research (Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan). A human colon carcinoma cell line, DLD-1 and DLD-1/FU (5-FU resistance), was kindly provided by Taiho Pharmaceutical.23 (link) Each cell line was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Nissui Pharmaceutical, Tokyo, Japan) and RPMI-1640 medium (Wako Pure Chemical) supplemented with 10% heat-inactivated fetal bovine serum (Japan Bioserum, Hiroshima, Japan), 100 units/mL penicillin, and 100 μg/mL streptomycin (ICN Biomedical, Aurora, OH, USA) in a 5% CO2/air incubator at 37°C, respectively.
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